The extent of parasite-associated necrosis in the placenta and foetal tissues of cattle following Neospora caninum infection in early and late gestation correlates with foetal death
The protozoan parasite Neospora caninum is the most frequently diagnosed abortifacient in the UK and a leading cause of abortion worldwide but the mechanisms leading to abortion are not fully understood. The distribution of parasites and the histopathological changes in the placenta and foetus were...
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| creator | Gibney, E. Helen Kipar, Anja Rosbottom, Anne Guy, Catherine S. Smith, Robert F. Hetzel, Udo Trees, Alexander J. Williams, Diana J.L. |
| description | The protozoan parasite
Neospora caninum is the most frequently diagnosed abortifacient in the UK and a leading cause of abortion worldwide but the mechanisms leading to abortion are not fully understood. The distribution of parasites and the histopathological changes in the placenta and foetus were compared in 12 cows following experimental infection of cattle with
N. caninum in early (
n
=
6) and late (
n
=
6) gestation, by PCR, immunohistology, light microscopy and transmission electron microscopy. Twelve uninfected pregnant cattle were used as controls. Infection in early gestation led to foetal death. In the placentae of cattle immediately following foetal death,
N. caninum DNA was detected and there was evidence of widespread parasite dissemination. This was associated with extensive focal epithelial necrosis, serum leakage and moderate maternal interstitial mononuclear cell infiltration. In the foetuses, parasites were evident in all tissues examined and were associated with necrosis. In the placenta of cattle infected in late gestation,
N. caninum DNA was detected sporadically but parasites were not evident immunohistologically. Small foci of necrosis were seen associated with mild interstitial mononuclear cell infiltration. Detection of
N. caninum DNA in the foetuses was sporadic and parasites were demonstrated immunohistologically in brain and spinal cord only, with an associated mononuclear cell infiltration. This data is consistent with uncontrolled parasite spread in an immunologically immature foetus and could, via multiparenchymal necrosis of foetal tissues or the widespread necrosis and inflammation observed in the placenta, be the cause of
Neospora-associated abortions. |
| doi_str_mv | 10.1016/j.ijpara.2007.09.015 |
| format | Article |
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Neospora caninum is the most frequently diagnosed abortifacient in the UK and a leading cause of abortion worldwide but the mechanisms leading to abortion are not fully understood. The distribution of parasites and the histopathological changes in the placenta and foetus were compared in 12 cows following experimental infection of cattle with
N. caninum in early (
n
=
6) and late (
n
=
6) gestation, by PCR, immunohistology, light microscopy and transmission electron microscopy. Twelve uninfected pregnant cattle were used as controls. Infection in early gestation led to foetal death. In the placentae of cattle immediately following foetal death,
N. caninum DNA was detected and there was evidence of widespread parasite dissemination. This was associated with extensive focal epithelial necrosis, serum leakage and moderate maternal interstitial mononuclear cell infiltration. In the foetuses, parasites were evident in all tissues examined and were associated with necrosis. In the placenta of cattle infected in late gestation,
N. caninum DNA was detected sporadically but parasites were not evident immunohistologically. Small foci of necrosis were seen associated with mild interstitial mononuclear cell infiltration. Detection of
N. caninum DNA in the foetuses was sporadic and parasites were demonstrated immunohistologically in brain and spinal cord only, with an associated mononuclear cell infiltration. This data is consistent with uncontrolled parasite spread in an immunologically immature foetus and could, via multiparenchymal necrosis of foetal tissues or the widespread necrosis and inflammation observed in the placenta, be the cause of
Neospora-associated abortions.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2007.09.015</identifier><identifier>PMID: 18021783</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>abortion (animals) ; Animals ; Biological and medical sciences ; Cattle ; Cattle Diseases - parasitology ; Cattle Diseases - pathology ; Coccidiosis - parasitology ; Coccidiosis - pathology ; Coccidiosis - veterinary ; cows ; DNA, Protozoan - analysis ; early gestation ; Epithelial Cells - parasitology ; Epithelial Cells - ultrastructure ; experimental infection ; Female ; fetal death ; Fetal Death - parasitology ; Fetal Death - pathology ; Fetal Death - veterinary ; fetal tissues ; fetus ; Fetus - parasitology ; Fetus - pathology ; Foetal death ; Fundamental and applied biological sciences. Psychology ; Gestational Age ; histopathology ; infection ; Inflammation ; Life cycle. Host-agent relationship. Pathogenesis ; Microscopy, Electron ; Necrosis ; Neospora - genetics ; Neospora - isolation & purification ; Neospora caninum ; neosporosis ; pathogenesis ; Placenta ; Placenta - parasitology ; Placenta - ultrastructure ; Polymerase Chain Reaction - methods ; Pregnancy ; Pregnancy Complications, Parasitic - parasitology ; Pregnancy Complications, Parasitic - pathology ; Pregnancy Complications, Parasitic - veterinary ; Protozoa ; tissue distribution ; transplacental transmission</subject><ispartof>International journal for parasitology, 2008-04, Vol.38 (5), p.579-588</ispartof><rights>2007 Australian Society for Parasitology Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-34c4a57dad6049627a93074f89ea97817a55b94eb642cc561addb79e90f79073</citedby><cites>FETCH-LOGICAL-c445t-34c4a57dad6049627a93074f89ea97817a55b94eb642cc561addb79e90f79073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpara.2007.09.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20158432$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18021783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gibney, E. Helen</creatorcontrib><creatorcontrib>Kipar, Anja</creatorcontrib><creatorcontrib>Rosbottom, Anne</creatorcontrib><creatorcontrib>Guy, Catherine S.</creatorcontrib><creatorcontrib>Smith, Robert F.</creatorcontrib><creatorcontrib>Hetzel, Udo</creatorcontrib><creatorcontrib>Trees, Alexander J.</creatorcontrib><creatorcontrib>Williams, Diana J.L.</creatorcontrib><title>The extent of parasite-associated necrosis in the placenta and foetal tissues of cattle following Neospora caninum infection in early and late gestation correlates with foetal death</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>The protozoan parasite
Neospora caninum is the most frequently diagnosed abortifacient in the UK and a leading cause of abortion worldwide but the mechanisms leading to abortion are not fully understood. The distribution of parasites and the histopathological changes in the placenta and foetus were compared in 12 cows following experimental infection of cattle with
N. caninum in early (
n
=
6) and late (
n
=
6) gestation, by PCR, immunohistology, light microscopy and transmission electron microscopy. Twelve uninfected pregnant cattle were used as controls. Infection in early gestation led to foetal death. In the placentae of cattle immediately following foetal death,
N. caninum DNA was detected and there was evidence of widespread parasite dissemination. This was associated with extensive focal epithelial necrosis, serum leakage and moderate maternal interstitial mononuclear cell infiltration. In the foetuses, parasites were evident in all tissues examined and were associated with necrosis. In the placenta of cattle infected in late gestation,
N. caninum DNA was detected sporadically but parasites were not evident immunohistologically. Small foci of necrosis were seen associated with mild interstitial mononuclear cell infiltration. Detection of
N. caninum DNA in the foetuses was sporadic and parasites were demonstrated immunohistologically in brain and spinal cord only, with an associated mononuclear cell infiltration. This data is consistent with uncontrolled parasite spread in an immunologically immature foetus and could, via multiparenchymal necrosis of foetal tissues or the widespread necrosis and inflammation observed in the placenta, be the cause of
Neospora-associated abortions.</description><subject>abortion (animals)</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - parasitology</subject><subject>Cattle Diseases - pathology</subject><subject>Coccidiosis - parasitology</subject><subject>Coccidiosis - pathology</subject><subject>Coccidiosis - veterinary</subject><subject>cows</subject><subject>DNA, Protozoan - analysis</subject><subject>early gestation</subject><subject>Epithelial Cells - parasitology</subject><subject>Epithelial Cells - ultrastructure</subject><subject>experimental infection</subject><subject>Female</subject><subject>fetal death</subject><subject>Fetal Death - parasitology</subject><subject>Fetal Death - pathology</subject><subject>Fetal Death - veterinary</subject><subject>fetal tissues</subject><subject>fetus</subject><subject>Fetus - parasitology</subject><subject>Fetus - pathology</subject><subject>Foetal death</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gestational Age</subject><subject>histopathology</subject><subject>infection</subject><subject>Inflammation</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Microscopy, Electron</subject><subject>Necrosis</subject><subject>Neospora - genetics</subject><subject>Neospora - isolation & purification</subject><subject>Neospora caninum</subject><subject>neosporosis</subject><subject>pathogenesis</subject><subject>Placenta</subject><subject>Placenta - parasitology</subject><subject>Placenta - ultrastructure</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Parasitic - parasitology</subject><subject>Pregnancy Complications, Parasitic - pathology</subject><subject>Pregnancy Complications, Parasitic - veterinary</subject><subject>Protozoa</subject><subject>tissue distribution</subject><subject>transplacental transmission</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAURiMEYobCGyDwBnYp17ETxxskNOJPGsGCsrZunZvWVWoX22WYB-P9cKYFdrCy5O_42L5fVT3lsOTAu1e7pdsdMOKyAVBL0Evg7b3qkvdK18BFe7-6BGigVi3XF9WjlHZQCCHlw-qC99Bw1YvL6udqS4x-ZPKZhZHNwuQy1ZhSsA4zDcyTjSG5xJxnudCHCW3BkaEf2Bgo48SyS-lIaVZYzHmiEkxTuHF-wz5RSIcQsSTe-eO-eEay2QU_GwnjdHunmsptbEMp411mQ4w07yV24_L2900DYd4-rh6MOCV6cl4X1erd29XVh_r68_uPV2-uaytlm2shrcRWDTh0IHXXKNQClBx7TahVzxW27VpLWneysbbtOA7DWmnSMCoNSiyqlyftIYZv5XvZ7F2yNE3oKRyTUSCaVrXwX7Dh0AnBZQHlCZxHmiKN5hDdHuOt4WDmWs3OnGo1c60GtJlLW1TPzv7jek_D30PnHgvw4gxgsjiNEb116Q_XFEkvRVO45yduxGBwEwvz9UtJBUCvBDRdIV6fCCpz_e4ommQdeUuDi6U1MwT377f-Au-Bz2E</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Gibney, E. Helen</creator><creator>Kipar, Anja</creator><creator>Rosbottom, Anne</creator><creator>Guy, Catherine S.</creator><creator>Smith, Robert F.</creator><creator>Hetzel, Udo</creator><creator>Trees, Alexander J.</creator><creator>Williams, Diana J.L.</creator><general>Elsevier Ltd</general><general>[Oxford; New York]: Elsevier Science</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20080401</creationdate><title>The extent of parasite-associated necrosis in the placenta and foetal tissues of cattle following Neospora caninum infection in early and late gestation correlates with foetal death</title><author>Gibney, E. Helen ; Kipar, Anja ; Rosbottom, Anne ; Guy, Catherine S. ; Smith, Robert F. ; Hetzel, Udo ; Trees, Alexander J. ; Williams, Diana J.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-34c4a57dad6049627a93074f89ea97817a55b94eb642cc561addb79e90f79073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>abortion (animals)</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle Diseases - parasitology</topic><topic>Cattle Diseases - pathology</topic><topic>Coccidiosis - parasitology</topic><topic>Coccidiosis - pathology</topic><topic>Coccidiosis - veterinary</topic><topic>cows</topic><topic>DNA, Protozoan - analysis</topic><topic>early gestation</topic><topic>Epithelial Cells - parasitology</topic><topic>Epithelial Cells - ultrastructure</topic><topic>experimental infection</topic><topic>Female</topic><topic>fetal death</topic><topic>Fetal Death - parasitology</topic><topic>Fetal Death - pathology</topic><topic>Fetal Death - veterinary</topic><topic>fetal tissues</topic><topic>fetus</topic><topic>Fetus - parasitology</topic><topic>Fetus - pathology</topic><topic>Foetal death</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gestational Age</topic><topic>histopathology</topic><topic>infection</topic><topic>Inflammation</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Microscopy, Electron</topic><topic>Necrosis</topic><topic>Neospora - genetics</topic><topic>Neospora - isolation & purification</topic><topic>Neospora caninum</topic><topic>neosporosis</topic><topic>pathogenesis</topic><topic>Placenta</topic><topic>Placenta - parasitology</topic><topic>Placenta - ultrastructure</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Parasitic - parasitology</topic><topic>Pregnancy Complications, Parasitic - pathology</topic><topic>Pregnancy Complications, Parasitic - veterinary</topic><topic>Protozoa</topic><topic>tissue distribution</topic><topic>transplacental transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gibney, E. Helen</creatorcontrib><creatorcontrib>Kipar, Anja</creatorcontrib><creatorcontrib>Rosbottom, Anne</creatorcontrib><creatorcontrib>Guy, Catherine S.</creatorcontrib><creatorcontrib>Smith, Robert F.</creatorcontrib><creatorcontrib>Hetzel, Udo</creatorcontrib><creatorcontrib>Trees, Alexander J.</creatorcontrib><creatorcontrib>Williams, Diana J.L.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gibney, E. Helen</au><au>Kipar, Anja</au><au>Rosbottom, Anne</au><au>Guy, Catherine S.</au><au>Smith, Robert F.</au><au>Hetzel, Udo</au><au>Trees, Alexander J.</au><au>Williams, Diana J.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The extent of parasite-associated necrosis in the placenta and foetal tissues of cattle following Neospora caninum infection in early and late gestation correlates with foetal death</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>38</volume><issue>5</issue><spage>579</spage><epage>588</epage><pages>579-588</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>The protozoan parasite
Neospora caninum is the most frequently diagnosed abortifacient in the UK and a leading cause of abortion worldwide but the mechanisms leading to abortion are not fully understood. The distribution of parasites and the histopathological changes in the placenta and foetus were compared in 12 cows following experimental infection of cattle with
N. caninum in early (
n
=
6) and late (
n
=
6) gestation, by PCR, immunohistology, light microscopy and transmission electron microscopy. Twelve uninfected pregnant cattle were used as controls. Infection in early gestation led to foetal death. In the placentae of cattle immediately following foetal death,
N. caninum DNA was detected and there was evidence of widespread parasite dissemination. This was associated with extensive focal epithelial necrosis, serum leakage and moderate maternal interstitial mononuclear cell infiltration. In the foetuses, parasites were evident in all tissues examined and were associated with necrosis. In the placenta of cattle infected in late gestation,
N. caninum DNA was detected sporadically but parasites were not evident immunohistologically. Small foci of necrosis were seen associated with mild interstitial mononuclear cell infiltration. Detection of
N. caninum DNA in the foetuses was sporadic and parasites were demonstrated immunohistologically in brain and spinal cord only, with an associated mononuclear cell infiltration. This data is consistent with uncontrolled parasite spread in an immunologically immature foetus and could, via multiparenchymal necrosis of foetal tissues or the widespread necrosis and inflammation observed in the placenta, be the cause of
Neospora-associated abortions.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18021783</pmid><doi>10.1016/j.ijpara.2007.09.015</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | International journal for parasitology, 2008-04, Vol.38 (5), p.579-588 |
| issn | 0020-7519 1879-0135 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70325750 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | abortion (animals) Animals Biological and medical sciences Cattle Cattle Diseases - parasitology Cattle Diseases - pathology Coccidiosis - parasitology Coccidiosis - pathology Coccidiosis - veterinary cows DNA, Protozoan - analysis early gestation Epithelial Cells - parasitology Epithelial Cells - ultrastructure experimental infection Female fetal death Fetal Death - parasitology Fetal Death - pathology Fetal Death - veterinary fetal tissues fetus Fetus - parasitology Fetus - pathology Foetal death Fundamental and applied biological sciences. Psychology Gestational Age histopathology infection Inflammation Life cycle. Host-agent relationship. Pathogenesis Microscopy, Electron Necrosis Neospora - genetics Neospora - isolation & purification Neospora caninum neosporosis pathogenesis Placenta Placenta - parasitology Placenta - ultrastructure Polymerase Chain Reaction - methods Pregnancy Pregnancy Complications, Parasitic - parasitology Pregnancy Complications, Parasitic - pathology Pregnancy Complications, Parasitic - veterinary Protozoa tissue distribution transplacental transmission |
| title | The extent of parasite-associated necrosis in the placenta and foetal tissues of cattle following Neospora caninum infection in early and late gestation correlates with foetal death |
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