Oligomerization of the potato virus X 25-kD movement protein
A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg²⁺-depende...
Gespeichert in:
| Veröffentlicht in: | Biochemistry (Moscow) 2008, Vol.73 (1), p.50-55 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 55 |
|---|---|
| container_issue | 1 |
| container_start_page | 50 |
| container_title | Biochemistry (Moscow) |
| container_volume | 73 |
| creator | Leshchiner, A. D Minina, E. A Rakitina, D. V Vishnichenko, V. K Solovyev, A. G Morozov, S. Yu Kalinina, N. O |
| description | A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg²⁺-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein. |
| doi_str_mv | 10.1134/s0006297908010070 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70325483</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70325483</sourcerecordid><originalsourceid>FETCH-LOGICAL-c362t-7dbea724a4f671a2dd83b9884bfbb40770ef538229696c3b0e7f3e22364c303a3</originalsourceid><addsrcrecordid>eNp9kE9Lw0AQxRdRbK1-AC-ak7fo7M4mmwUvUv9CoYda8LZskk1NTbJ1NynopzclBcGDp2F4v_eGeYScU7imFPmNB4CYSSEhAQog4ICMaQxJiMDhkIx3crjTR-TE-3W_MpB4TEY0YZJTJsfkdl6VK1sbV37rtrRNYIugfTfBxra6tcG2dJ0P3gIWhR_3QW23pjZNG2ycbU3ZnJKjQlfenO3nhCwfH16nz-Fs_vQyvZuFGcasDUWeGi0Y17yIBdUszxNMZZLwtEhTDkKAKSJMGJOxjDNMwYgCDWMY8wwBNU7I1ZDb3_3sjG9VXfrMVJVujO28EoAs4gn2IB3AzFnvnSnUxpW1dl-KgtpVphZ_K-s9F_vwLq1N_uvYd9QDbAB8LzUr49Tadq7pH_439XIwFdoqvXKlV8sFA4pAJUZCMPwBsgh9Jw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70325483</pqid></control><display><type>article</type><title>Oligomerization of the potato virus X 25-kD movement protein</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Leshchiner, A. D ; Minina, E. A ; Rakitina, D. V ; Vishnichenko, V. K ; Solovyev, A. G ; Morozov, S. Yu ; Kalinina, N. O</creator><creatorcontrib>Leshchiner, A. D ; Minina, E. A ; Rakitina, D. V ; Vishnichenko, V. K ; Solovyev, A. G ; Morozov, S. Yu ; Kalinina, N. O</creatorcontrib><description>A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg²⁺-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/s0006297908010070</identifier><identifier>PMID: 18294129</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Dimerization ; Glutaral - chemistry ; Life Sciences ; Microbiology ; Plant Viral Movement Proteins - chemistry ; Plant Viral Movement Proteins - metabolism ; Potexvirus ; Two-Hybrid System Techniques ; Ultracentrifugation</subject><ispartof>Biochemistry (Moscow), 2008, Vol.73 (1), p.50-55</ispartof><rights>Pleiades Publishing, Ltd. 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-7dbea724a4f671a2dd83b9884bfbb40770ef538229696c3b0e7f3e22364c303a3</citedby><cites>FETCH-LOGICAL-c362t-7dbea724a4f671a2dd83b9884bfbb40770ef538229696c3b0e7f3e22364c303a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297908010070$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297908010070$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,4010,27904,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18294129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leshchiner, A. D</creatorcontrib><creatorcontrib>Minina, E. A</creatorcontrib><creatorcontrib>Rakitina, D. V</creatorcontrib><creatorcontrib>Vishnichenko, V. K</creatorcontrib><creatorcontrib>Solovyev, A. G</creatorcontrib><creatorcontrib>Morozov, S. Yu</creatorcontrib><creatorcontrib>Kalinina, N. O</creatorcontrib><title>Oligomerization of the potato virus X 25-kD movement protein</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg²⁺-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Dimerization</subject><subject>Glutaral - chemistry</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Plant Viral Movement Proteins - chemistry</subject><subject>Plant Viral Movement Proteins - metabolism</subject><subject>Potexvirus</subject><subject>Two-Hybrid System Techniques</subject><subject>Ultracentrifugation</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9Lw0AQxRdRbK1-AC-ak7fo7M4mmwUvUv9CoYda8LZskk1NTbJ1NynopzclBcGDp2F4v_eGeYScU7imFPmNB4CYSSEhAQog4ICMaQxJiMDhkIx3crjTR-TE-3W_MpB4TEY0YZJTJsfkdl6VK1sbV37rtrRNYIugfTfBxra6tcG2dJ0P3gIWhR_3QW23pjZNG2ycbU3ZnJKjQlfenO3nhCwfH16nz-Fs_vQyvZuFGcasDUWeGi0Y17yIBdUszxNMZZLwtEhTDkKAKSJMGJOxjDNMwYgCDWMY8wwBNU7I1ZDb3_3sjG9VXfrMVJVujO28EoAs4gn2IB3AzFnvnSnUxpW1dl-KgtpVphZ_K-s9F_vwLq1N_uvYd9QDbAB8LzUr49Tadq7pH_439XIwFdoqvXKlV8sFA4pAJUZCMPwBsgh9Jw</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Leshchiner, A. D</creator><creator>Minina, E. A</creator><creator>Rakitina, D. V</creator><creator>Vishnichenko, V. K</creator><creator>Solovyev, A. G</creator><creator>Morozov, S. Yu</creator><creator>Kalinina, N. O</creator><general>Dordrecht : SP MAIK Nauka/Interperiodica</general><general>SP MAIK Nauka/Interperiodica</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Oligomerization of the potato virus X 25-kD movement protein</title><author>Leshchiner, A. D ; Minina, E. A ; Rakitina, D. V ; Vishnichenko, V. K ; Solovyev, A. G ; Morozov, S. Yu ; Kalinina, N. O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-7dbea724a4f671a2dd83b9884bfbb40770ef538229696c3b0e7f3e22364c303a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Dimerization</topic><topic>Glutaral - chemistry</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Plant Viral Movement Proteins - chemistry</topic><topic>Plant Viral Movement Proteins - metabolism</topic><topic>Potexvirus</topic><topic>Two-Hybrid System Techniques</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leshchiner, A. D</creatorcontrib><creatorcontrib>Minina, E. A</creatorcontrib><creatorcontrib>Rakitina, D. V</creatorcontrib><creatorcontrib>Vishnichenko, V. K</creatorcontrib><creatorcontrib>Solovyev, A. G</creatorcontrib><creatorcontrib>Morozov, S. Yu</creatorcontrib><creatorcontrib>Kalinina, N. O</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Moscow)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leshchiner, A. D</au><au>Minina, E. A</au><au>Rakitina, D. V</au><au>Vishnichenko, V. K</au><au>Solovyev, A. G</au><au>Morozov, S. Yu</au><au>Kalinina, N. O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oligomerization of the potato virus X 25-kD movement protein</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2008</date><risdate>2008</risdate><volume>73</volume><issue>1</issue><spage>50</spage><epage>55</epage><pages>50-55</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg²⁺-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.</abstract><cop>Dordrecht</cop><pub>Dordrecht : SP MAIK Nauka/Interperiodica</pub><pmid>18294129</pmid><doi>10.1134/s0006297908010070</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2979 |
| ispartof | Biochemistry (Moscow), 2008, Vol.73 (1), p.50-55 |
| issn | 0006-2979 1608-3040 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70325483 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Dimerization Glutaral - chemistry Life Sciences Microbiology Plant Viral Movement Proteins - chemistry Plant Viral Movement Proteins - metabolism Potexvirus Two-Hybrid System Techniques Ultracentrifugation |
| title | Oligomerization of the potato virus X 25-kD movement protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T20%3A52%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Oligomerization%20of%20the%20potato%20virus%20X%2025-kD%20movement%20protein&rft.jtitle=Biochemistry%20(Moscow)&rft.au=Leshchiner,%20A.%20D&rft.date=2008&rft.volume=73&rft.issue=1&rft.spage=50&rft.epage=55&rft.pages=50-55&rft.issn=0006-2979&rft.eissn=1608-3040&rft_id=info:doi/10.1134/s0006297908010070&rft_dat=%3Cproquest_cross%3E70325483%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70325483&rft_id=info:pmid/18294129&rfr_iscdi=true |