Detection of HIV Type 1 Gag-Specific CD4+ T Cell Responses in Acutely Infected Infants
Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TN...
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| Veröffentlicht in: | AIDS research and human retroviruses 2008-02, Vol.24 (2), p.265-270 |
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| creator | RAMDUTH, Danni THOBAKGALE, Christina F PRENDERGAST, Andrew TUDOR-WILLIAMS, Gareth DONG, Krista JEENA, Prakash COOVADIA, Hoosen M DAY, Cheryl L KIEPIELA, Photini GOULDER, Philip J. R WALKER, Bruce D MKHWANAZI, Nompumelelo P DE PIERRES, Chantal REDDY, Sharon VAN DER STOK, Mary MNCUBE, Zenele MPHATSWE, Wendy BLANCKENBERG, Natasha CENGIMBO, Ayanda |
| description | Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was |
| doi_str_mv | 10.1089/aid.2007.0096 |
| format | Article |
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R ; WALKER, Bruce D ; MKHWANAZI, Nompumelelo P ; DE PIERRES, Chantal ; REDDY, Sharon ; VAN DER STOK, Mary ; MNCUBE, Zenele ; MPHATSWE, Wendy ; BLANCKENBERG, Natasha ; CENGIMBO, Ayanda</creator><creatorcontrib>RAMDUTH, Danni ; THOBAKGALE, Christina F ; PRENDERGAST, Andrew ; TUDOR-WILLIAMS, Gareth ; DONG, Krista ; JEENA, Prakash ; COOVADIA, Hoosen M ; DAY, Cheryl L ; KIEPIELA, Photini ; GOULDER, Philip J. R ; WALKER, Bruce D ; MKHWANAZI, Nompumelelo P ; DE PIERRES, Chantal ; REDDY, Sharon ; VAN DER STOK, Mary ; MNCUBE, Zenele ; MPHATSWE, Wendy ; BLANCKENBERG, Natasha ; CENGIMBO, Ayanda</creatorcontrib><description>Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. Among untreated infants HIV-specific CD4(+) responses were detected sporadically indicating a dysfunctional immune response in the face of constant exposure to high levels of viremia.</description><identifier>ISSN: 0889-2229</identifier><identifier>EISSN: 1931-8405</identifier><identifier>DOI: 10.1089/aid.2007.0096</identifier><identifier>PMID: 18284325</identifier><identifier>CODEN: ARHRE7</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>AIDS/HIV ; Animals ; Biological and medical sciences ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes - immunology ; Cell Proliferation ; Cells, Cultured ; Fundamental and applied biological sciences. Psychology ; gag Gene Products, Human Immunodeficiency Virus - immunology ; HIV Infections - immunology ; HIV-1 - immunology ; Human viral diseases ; Humans ; Infant ; Infectious diseases ; Interferon-gamma - biosynthesis ; Interleukin-2 - biosynthesis ; Longitudinal Studies ; Medical sciences ; Microbiology ; Miscellaneous ; Tumor Necrosis Factor-alpha - biosynthesis ; Viral diseases ; Viral Load ; Virology</subject><ispartof>AIDS research and human retroviruses, 2008-02, Vol.24 (2), p.265-270</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-a6cb10fc89a76ccfd046721236c9007108ad61d2d87541d44ad94b8215e672a53</citedby><cites>FETCH-LOGICAL-c321t-a6cb10fc89a76ccfd046721236c9007108ad61d2d87541d44ad94b8215e672a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3043,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20161452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18284325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RAMDUTH, Danni</creatorcontrib><creatorcontrib>THOBAKGALE, Christina F</creatorcontrib><creatorcontrib>PRENDERGAST, Andrew</creatorcontrib><creatorcontrib>TUDOR-WILLIAMS, Gareth</creatorcontrib><creatorcontrib>DONG, Krista</creatorcontrib><creatorcontrib>JEENA, Prakash</creatorcontrib><creatorcontrib>COOVADIA, Hoosen M</creatorcontrib><creatorcontrib>DAY, Cheryl L</creatorcontrib><creatorcontrib>KIEPIELA, Photini</creatorcontrib><creatorcontrib>GOULDER, Philip J. R</creatorcontrib><creatorcontrib>WALKER, Bruce D</creatorcontrib><creatorcontrib>MKHWANAZI, Nompumelelo P</creatorcontrib><creatorcontrib>DE PIERRES, Chantal</creatorcontrib><creatorcontrib>REDDY, Sharon</creatorcontrib><creatorcontrib>VAN DER STOK, Mary</creatorcontrib><creatorcontrib>MNCUBE, Zenele</creatorcontrib><creatorcontrib>MPHATSWE, Wendy</creatorcontrib><creatorcontrib>BLANCKENBERG, Natasha</creatorcontrib><creatorcontrib>CENGIMBO, Ayanda</creatorcontrib><title>Detection of HIV Type 1 Gag-Specific CD4+ T Cell Responses in Acutely Infected Infants</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. 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Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. Among untreated infants HIV-specific CD4(+) responses were detected sporadically indicating a dysfunctional immune response in the face of constant exposure to high levels of viremia.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>18284325</pmid><doi>10.1089/aid.2007.0096</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0889-2229 |
| ispartof | AIDS research and human retroviruses, 2008-02, Vol.24 (2), p.265-270 |
| issn | 0889-2229 1931-8405 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70319349 |
| source | Mary Ann Liebert Online Subscription; MEDLINE; Alma/SFX Local Collection |
| subjects | AIDS/HIV Animals Biological and medical sciences CD4 Lymphocyte Count CD4-Positive T-Lymphocytes - immunology Cell Proliferation Cells, Cultured Fundamental and applied biological sciences. Psychology gag Gene Products, Human Immunodeficiency Virus - immunology HIV Infections - immunology HIV-1 - immunology Human viral diseases Humans Infant Infectious diseases Interferon-gamma - biosynthesis Interleukin-2 - biosynthesis Longitudinal Studies Medical sciences Microbiology Miscellaneous Tumor Necrosis Factor-alpha - biosynthesis Viral diseases Viral Load Virology |
| title | Detection of HIV Type 1 Gag-Specific CD4+ T Cell Responses in Acutely Infected Infants |
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