Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells
Vacuole membrane protein 1 (Vmp1) is described as a cancer-relevant cell cycle modulator, but the function of this protein and its mode of action in tumor progression are still unknown. In this study, we show that the VMP1 mRNA level is significantly reduced in kidney cancer metastases as compared t...
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| Veröffentlicht in: | Oncogene 2008-02, Vol.27 (9), p.1320-1326 |
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| container_title | Oncogene |
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| creator | Sauermann, M Sahin, Ö Sültmann, H Hahne, F Blaszkiewicz, S Majety, M Zatloukal, K Füzesi, L Poustka, A Wiemann, S Arlt, D |
| description | Vacuole membrane protein 1 (Vmp1) is described as a cancer-relevant cell cycle modulator, but the function of this protein and its mode of action in tumor progression are still unknown. In this study, we show that the VMP1 mRNA level is significantly reduced in kidney cancer metastases as compared to primary tumors. Further, VMP1 expression is also decreased in the invasive breast cancer cell lines HCC1954 and MDA-MB-231 as compared to the non-invasive cell lines MCF-12A, T-47D and MCF-7. We show for the first time that Vmp1 is a plasma membrane protein and an essential component of initial cell–cell contacts and tight junction formation. It interacts with the tight junction protein Zonula Occludens-1 and colocalizes in spots between neighboring HEK293 cells. Downregulation of VMP1 by RNAi results in loss of cell adherence, and increases the invasion capacity of the non-invasive kidney cancer cell line Caki-2. In conclusion, our findings establish Vmp1 to be a novel cell–cell adhesion protein and that its expression level determines the invasion and metastatic potential of cancer cells. |
| doi_str_mv | 10.1038/sj.onc.1210743 |
| format | Article |
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In conclusion, our findings establish Vmp1 to be a novel cell–cell adhesion protein and that its expression level determines the invasion and metastatic potential of cancer cells.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1210743</identifier><identifier>PMID: 17724469</identifier><identifier>CODEN: ONCNES</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Apoptosis ; Biological and medical sciences ; Breast cancer ; Breast Neoplasms - chemistry ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cancer ; Cancer cells ; Carcinoma, Ductal, Breast - chemistry ; Carcinoma, Ductal, Breast - genetics ; Carcinoma, Ductal, Breast - pathology ; Cell adhesion ; Cell adhesion & migration ; Cell Adhesion - genetics ; Cell Biology ; Cell Communication - genetics ; Cell cycle ; Cell interaction ; Cell Line ; Cell Line, Tumor ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cells ; Cercopithecus aethiops ; COS Cells ; Female ; Fundamental and applied biological sciences. Psychology ; Human Genetics ; Humans ; Internal Medicine ; Kidney cancer ; Kidney Neoplasms - chemistry ; Kidney Neoplasms - genetics ; Kidney Neoplasms - pathology ; Kidneys ; Medicine & Public Health ; Membrane proteins ; Membrane Proteins - antagonists & inhibitors ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Membranes ; Metastases ; Molecular and cellular biology ; mRNA ; Neoplasm Invasiveness ; Oncology ; Physiological aspects ; Proteins ; Ribonucleic acid ; RNA ; RNA-mediated interference ; short-communication ; Tight Junctions - chemistry ; Tight Junctions - metabolism ; Tight Junctions - pathology ; Tumor cell lines ; Tumor cells ; Tumor Cells, Cultured ; Vacuoles - chemistry ; Vacuoles - pathology ; Zonula occludens-1 protein</subject><ispartof>Oncogene, 2008-02, Vol.27 (9), p.1320-1326</ispartof><rights>Springer Nature Limited 2008</rights><rights>2008 INIST-CNRS</rights><rights>COPYRIGHT 2008 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 21, 2008</rights><rights>Nature Publishing Group 2008.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-60fe39612abb1fbd2c7064b66e63cdd5166e3fbc584d62b347579a306b15db4a3</citedby><cites>FETCH-LOGICAL-c556t-60fe39612abb1fbd2c7064b66e63cdd5166e3fbc584d62b347579a306b15db4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/sj.onc.1210743$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/sj.onc.1210743$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20178187$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17724469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sauermann, M</creatorcontrib><creatorcontrib>Sahin, Ö</creatorcontrib><creatorcontrib>Sültmann, H</creatorcontrib><creatorcontrib>Hahne, F</creatorcontrib><creatorcontrib>Blaszkiewicz, S</creatorcontrib><creatorcontrib>Majety, M</creatorcontrib><creatorcontrib>Zatloukal, K</creatorcontrib><creatorcontrib>Füzesi, L</creatorcontrib><creatorcontrib>Poustka, A</creatorcontrib><creatorcontrib>Wiemann, S</creatorcontrib><creatorcontrib>Arlt, D</creatorcontrib><title>Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells</title><title>Oncogene</title><addtitle>Oncogene</addtitle><addtitle>Oncogene</addtitle><description>Vacuole membrane protein 1 (Vmp1) is described as a cancer-relevant cell cycle modulator, but the function of this protein and its mode of action in tumor progression are still unknown. In this study, we show that the VMP1 mRNA level is significantly reduced in kidney cancer metastases as compared to primary tumors. Further, VMP1 expression is also decreased in the invasive breast cancer cell lines HCC1954 and MDA-MB-231 as compared to the non-invasive cell lines MCF-12A, T-47D and MCF-7. We show for the first time that Vmp1 is a plasma membrane protein and an essential component of initial cell–cell contacts and tight junction formation. It interacts with the tight junction protein Zonula Occludens-1 and colocalizes in spots between neighboring HEK293 cells. Downregulation of VMP1 by RNAi results in loss of cell adherence, and increases the invasion capacity of the non-invasive kidney cancer cell line Caki-2. 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Action of oncogenes and antioncogenes</subject><subject>Cells</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Kidney cancer</subject><subject>Kidney Neoplasms - chemistry</subject><subject>Kidney Neoplasms - genetics</subject><subject>Kidney Neoplasms - pathology</subject><subject>Kidneys</subject><subject>Medicine & Public Health</subject><subject>Membrane proteins</subject><subject>Membrane Proteins - antagonists & inhibitors</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Membranes</subject><subject>Metastases</subject><subject>Molecular and cellular biology</subject><subject>mRNA</subject><subject>Neoplasm Invasiveness</subject><subject>Oncology</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>short-communication</subject><subject>Tight Junctions - chemistry</subject><subject>Tight Junctions - metabolism</subject><subject>Tight Junctions - pathology</subject><subject>Tumor cell lines</subject><subject>Tumor cells</subject><subject>Tumor Cells, Cultured</subject><subject>Vacuoles - chemistry</subject><subject>Vacuoles - pathology</subject><subject>Zonula occludens-1 protein</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFks1rFTEUxYMo9lndupSg6G5ec5NJMlmW4hcUBNGlhEzmps5jZvJMZkr735tnBx9Ii2SRkPzOveeSQ8hLYFtgojnLu22c_BY4MF2LR2QDtVaVlKZ-TDbMSFYZLvgJeZbzjjGmDeNPyQlozetamQ358RW7xWNH8WafMOc-TjQGeu38EgekI45tchPSfYoz9hMF6kJAP2c6_0TaT9fuj8S7vfP9fHvQzssYE_U4DPk5eRLckPHFup-S7x_ef7v4VF1--fj54vyy8lKquVIsoDAKuGtbCG3HvWaqbpVCJXzXSSgnEVovm7pTvBW1lto4wVQLsmtrJ07Ju7u6xeavBfNsxz4fHBTrcclWMwGKc_ZfEIwWxkhVwDf_gLu4pKkMYbmqQRiQ0BTq9YMU10JBY8yx1JUb0PZTiHNy_tDXnoMp32aU5IXa3kOV1eHY-zhh6Mv9fQKfYs4Jg92nfnTp1gKzh2zYvLMlG3bNRhG8Ws0u7YjdEV_DUIC3K-Cyd0MoH-_7_JfjDHQDjS7c2R2Xy9N0hek49QOtfwMDu8-M</recordid><startdate>20080221</startdate><enddate>20080221</enddate><creator>Sauermann, M</creator><creator>Sahin, Ö</creator><creator>Sültmann, H</creator><creator>Hahne, F</creator><creator>Blaszkiewicz, S</creator><creator>Majety, M</creator><creator>Zatloukal, K</creator><creator>Füzesi, L</creator><creator>Poustka, A</creator><creator>Wiemann, S</creator><creator>Arlt, D</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080221</creationdate><title>Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells</title><author>Sauermann, M ; Sahin, Ö ; Sültmann, H ; Hahne, F ; Blaszkiewicz, S ; Majety, M ; Zatloukal, K ; Füzesi, L ; Poustka, A ; Wiemann, S ; Arlt, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-60fe39612abb1fbd2c7064b66e63cdd5166e3fbc584d62b347579a306b15db4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer</topic><topic>Cancer cells</topic><topic>Carcinoma, Ductal, Breast - chemistry</topic><topic>Carcinoma, Ductal, Breast - genetics</topic><topic>Carcinoma, Ductal, Breast - pathology</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell Adhesion - genetics</topic><topic>Cell Biology</topic><topic>Cell Communication - genetics</topic><topic>Cell cycle</topic><topic>Cell interaction</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cells</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Kidney cancer</topic><topic>Kidney Neoplasms - chemistry</topic><topic>Kidney Neoplasms - genetics</topic><topic>Kidney Neoplasms - pathology</topic><topic>Kidneys</topic><topic>Medicine & Public Health</topic><topic>Membrane proteins</topic><topic>Membrane Proteins - antagonists & inhibitors</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Membranes</topic><topic>Metastases</topic><topic>Molecular and cellular biology</topic><topic>mRNA</topic><topic>Neoplasm Invasiveness</topic><topic>Oncology</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>short-communication</topic><topic>Tight Junctions - chemistry</topic><topic>Tight Junctions - metabolism</topic><topic>Tight Junctions - pathology</topic><topic>Tumor cell lines</topic><topic>Tumor cells</topic><topic>Tumor Cells, Cultured</topic><topic>Vacuoles - 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In this study, we show that the VMP1 mRNA level is significantly reduced in kidney cancer metastases as compared to primary tumors. Further, VMP1 expression is also decreased in the invasive breast cancer cell lines HCC1954 and MDA-MB-231 as compared to the non-invasive cell lines MCF-12A, T-47D and MCF-7. We show for the first time that Vmp1 is a plasma membrane protein and an essential component of initial cell–cell contacts and tight junction formation. It interacts with the tight junction protein Zonula Occludens-1 and colocalizes in spots between neighboring HEK293 cells. Downregulation of VMP1 by RNAi results in loss of cell adherence, and increases the invasion capacity of the non-invasive kidney cancer cell line Caki-2. In conclusion, our findings establish Vmp1 to be a novel cell–cell adhesion protein and that its expression level determines the invasion and metastatic potential of cancer cells.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>17724469</pmid><doi>10.1038/sj.onc.1210743</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Biological and medical sciences Breast cancer Breast Neoplasms - chemistry Breast Neoplasms - genetics Breast Neoplasms - pathology Cancer Cancer cells Carcinoma, Ductal, Breast - chemistry Carcinoma, Ductal, Breast - genetics Carcinoma, Ductal, Breast - pathology Cell adhesion Cell adhesion & migration Cell Adhesion - genetics Cell Biology Cell Communication - genetics Cell cycle Cell interaction Cell Line Cell Line, Tumor Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cells Cercopithecus aethiops COS Cells Female Fundamental and applied biological sciences. Psychology Human Genetics Humans Internal Medicine Kidney cancer Kidney Neoplasms - chemistry Kidney Neoplasms - genetics Kidney Neoplasms - pathology Kidneys Medicine & Public Health Membrane proteins Membrane Proteins - antagonists & inhibitors Membrane Proteins - biosynthesis Membrane Proteins - genetics Membranes Metastases Molecular and cellular biology mRNA Neoplasm Invasiveness Oncology Physiological aspects Proteins Ribonucleic acid RNA RNA-mediated interference short-communication Tight Junctions - chemistry Tight Junctions - metabolism Tight Junctions - pathology Tumor cell lines Tumor cells Tumor Cells, Cultured Vacuoles - chemistry Vacuoles - pathology Zonula occludens-1 protein |
| title | Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells |
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