Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures

We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690/F740 ratio as a...

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Veröffentlicht in:	Applied Optics 2007-04, Vol.46 (11), p.2138-2142
Hauptverfasser:	Muñoz-Muñoz, Ana Celia, Gutiérrez-Pulido, Humberto, Rodríguez-Domínguez, José Manuel, Gutiérrez-Mora, Antonia, Rodríguez-Garay, Benjamín, Cervantes-Martínez, Jesús
Format:	Artikel
Sprache:	eng
Schlagworte:	Cefotaxime - pharmacology Lasers Magnoliopsida - drug effects Magnoliopsida - metabolism Organogenesis - drug effects Organogenesis - physiology Plant Leaves - drug effects Plant Leaves - metabolism Plant Proteins - analysis Plant Proteins - metabolism Spectrometry, Fluorescence - methods
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Beschreibung
Zusammenfassung:	We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690/F740 ratio as a result of the stress originated in the organogenic explants due to shoot emergence, and the relationship between fluorescence spectra and shoot development were detected by LIF through closed containers of Saintpaulia ionantha.
ISSN:	1559-128X 0003-6935 1539-4522
DOI:	10.1364/AO.46.002138