Molecular characterization of the env gene from Brazilian field isolates of Bovine Leukemia Virus
Molecular characterization of Bovine leukemia virus (BLV) isolates from Brazil using the env gene sequences revealed a high conservation of this gene. In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization doma...
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| Veröffentlicht in: | Virus genes 2007-06, Vol.34 (3), p.343-350 |
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| creator | Camargos, Marcelo Fernandes Pereda, Ariel Stancek, Daniel Rocha, Maurílio Andrade Reis, Jenner Karlisson Pimenta dos Greiser-Wilke, Irene Leite, Rômulo Cerqueira |
| description | Molecular characterization of Bovine leukemia virus (BLV) isolates from Brazil using the env gene sequences revealed a high conservation of this gene. In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization domains and other critical residues involved with the envelope structural domains and viral infectivity were conserved. Most of the substitutions found in the aminoacid sequences of the gp51 protein were localized in the G and H epitopes. Using the SIFT software, it was predicted that they should not alter the protein functions. Phylogenetic analyses showed that partial or complete env gene sequences grouped in three or four phylogenetic clusters, respectively. The sequences from the Brazilian isolates had similar mutation rates as compared to samples from other countries, and belonged to at least two phylogenetic clusters. |
| doi_str_mv | 10.1007/s11262-006-0011-x |
| format | Article |
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In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization domains and other critical residues involved with the envelope structural domains and viral infectivity were conserved. Most of the substitutions found in the aminoacid sequences of the gp51 protein were localized in the G and H epitopes. Using the SIFT software, it was predicted that they should not alter the protein functions. Phylogenetic analyses showed that partial or complete env gene sequences grouped in three or four phylogenetic clusters, respectively. The sequences from the Brazilian isolates had similar mutation rates as compared to samples from other countries, and belonged to at least two phylogenetic clusters.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-006-0011-x</identifier><identifier>PMID: 16917740</identifier><language>eng</language><publisher>United States: New York : Kluwer Academic Publishers-Plenum Publishers</publisher><subject>Amino Acid Sequence ; Animals ; Bovine leukemia virus ; Brazil ; Cattle ; Deltaretrovirus ; DNA Restriction Enzymes - metabolism ; env gene ; Genes, env ; Leukemia Virus, Bovine - genetics ; Leukemia Virus, Bovine - isolation & purification ; Molecular characterization ; Molecular Sequence Data ; Phylogenetic analysis ; Phylogeny ; Polymorphism, Restriction Fragment Length ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid</subject><ispartof>Virus genes, 2007-06, Vol.34 (3), p.343-350</ispartof><rights>Springer Science+Business Media, LLC 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-be2b732189f3888f83213da16a58a852d965e3d3882d4091ba45b3ed74f4c3023</citedby><cites>FETCH-LOGICAL-c490t-be2b732189f3888f83213da16a58a852d965e3d3882d4091ba45b3ed74f4c3023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16917740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Camargos, Marcelo Fernandes</creatorcontrib><creatorcontrib>Pereda, Ariel</creatorcontrib><creatorcontrib>Stancek, Daniel</creatorcontrib><creatorcontrib>Rocha, Maurílio Andrade</creatorcontrib><creatorcontrib>Reis, Jenner Karlisson Pimenta dos</creatorcontrib><creatorcontrib>Greiser-Wilke, Irene</creatorcontrib><creatorcontrib>Leite, Rômulo Cerqueira</creatorcontrib><title>Molecular characterization of the env gene from Brazilian field isolates of Bovine Leukemia Virus</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><description>Molecular characterization of Bovine leukemia virus (BLV) isolates from Brazil using the env gene sequences revealed a high conservation of this gene. 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The sequences from the Brazilian isolates had similar mutation rates as compared to samples from other countries, and belonged to at least two phylogenetic clusters.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bovine leukemia virus</subject><subject>Brazil</subject><subject>Cattle</subject><subject>Deltaretrovirus</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>env gene</subject><subject>Genes, env</subject><subject>Leukemia Virus, Bovine - genetics</subject><subject>Leukemia Virus, Bovine - isolation & purification</subject><subject>Molecular characterization</subject><subject>Molecular Sequence Data</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1rFTEUhoMo9lr9AW40uOhu9ORzkqUtVoUrLrTiLmRmTtrUmUmbzJTaX28u94LgxkVIIM_7cg4PIS8ZvGUA7bvCGNe8AdD1MNbcPyIbplreWCt_PiYbsBwao7Q9Is9KuQYAY7h8So6YtqxtJWyI_5JG7NfRZ9pf-ez7BXN88EtMM02BLldIcb6jlzgjDTlN9DT7hzhGP9MQcRxoLGn0C5YdfZruYuW2uP7CKXr6I-a1PCdPgh8Lvjjcx-Ti_MP3s0_N9uvHz2fvt00vLSxNh7xrBWfGBmGMCaa-xeCZ9sp4o_hgtUIx1D8-SLCs81J1AodWBtkL4OKYnOx7b3K6XbEsboqlx3H0M6a1uBYEkxzEf0FmNXArVQXf_ANepzXPdQnHudBaKGUqxPZQn1MpGYO7yXHy-bdj4HaW3N6Sq5bczpK7r5lXh-K1m3D4mzhoqcDrPRB8cv4yx-IuvnFgovap3WjiD1QBlbY</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Camargos, Marcelo Fernandes</creator><creator>Pereda, Ariel</creator><creator>Stancek, Daniel</creator><creator>Rocha, Maurílio Andrade</creator><creator>Reis, Jenner Karlisson Pimenta dos</creator><creator>Greiser-Wilke, Irene</creator><creator>Leite, Rômulo Cerqueira</creator><general>New York : Kluwer Academic Publishers-Plenum Publishers</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070601</creationdate><title>Molecular characterization of the env gene from Brazilian field isolates of Bovine Leukemia Virus</title><author>Camargos, Marcelo Fernandes ; Pereda, Ariel ; Stancek, Daniel ; Rocha, Maurílio Andrade ; Reis, Jenner Karlisson Pimenta dos ; Greiser-Wilke, Irene ; Leite, Rômulo Cerqueira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-be2b732189f3888f83213da16a58a852d965e3d3882d4091ba45b3ed74f4c3023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bovine leukemia virus</topic><topic>Brazil</topic><topic>Cattle</topic><topic>Deltaretrovirus</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>env gene</topic><topic>Genes, env</topic><topic>Leukemia Virus, Bovine - genetics</topic><topic>Leukemia Virus, Bovine - isolation & purification</topic><topic>Molecular characterization</topic><topic>Molecular Sequence Data</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Camargos, Marcelo Fernandes</creatorcontrib><creatorcontrib>Pereda, Ariel</creatorcontrib><creatorcontrib>Stancek, Daniel</creatorcontrib><creatorcontrib>Rocha, Maurílio Andrade</creatorcontrib><creatorcontrib>Reis, Jenner Karlisson Pimenta dos</creatorcontrib><creatorcontrib>Greiser-Wilke, Irene</creatorcontrib><creatorcontrib>Leite, Rômulo Cerqueira</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - 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In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization domains and other critical residues involved with the envelope structural domains and viral infectivity were conserved. Most of the substitutions found in the aminoacid sequences of the gp51 protein were localized in the G and H epitopes. Using the SIFT software, it was predicted that they should not alter the protein functions. Phylogenetic analyses showed that partial or complete env gene sequences grouped in three or four phylogenetic clusters, respectively. The sequences from the Brazilian isolates had similar mutation rates as compared to samples from other countries, and belonged to at least two phylogenetic clusters.</abstract><cop>United States</cop><pub>New York : Kluwer Academic Publishers-Plenum Publishers</pub><pmid>16917740</pmid><doi>10.1007/s11262-006-0011-x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virus genes, 2007-06, Vol.34 (3), p.343-350 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acid Sequence Animals Bovine leukemia virus Brazil Cattle Deltaretrovirus DNA Restriction Enzymes - metabolism env gene Genes, env Leukemia Virus, Bovine - genetics Leukemia Virus, Bovine - isolation & purification Molecular characterization Molecular Sequence Data Phylogenetic analysis Phylogeny Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Sequence Homology, Amino Acid |
| title | Molecular characterization of the env gene from Brazilian field isolates of Bovine Leukemia Virus |
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