Evaluation of the 1-methyl-2-phenylindole colorimetric assay for aldehydic lipid peroxidation products in plants: Malondialdehyde and 4-hydroxynonenal

The 1-methyl-2-phenylindole colorimetric assay is considered specific for malondialdehyde (MDA) and 4-hydroxynonenal (HNE) in mammalian systems, but its specificity in plant tissues is unknown. This study demonstrates that the assay produces a purple/blue chromophore with an absorbance peak at 586 n...

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Veröffentlicht in:	Plant physiology and biochemistry 2007-02, Vol.45 (2), p.108-112
Hauptverfasser:	Johnston, Jason W., Horne, Susan, Harding, Keith, Benson, Erica E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehydes - analysis Aldehydes - chemistry Aldehydes - metabolism Beta vulgaris - metabolism Biochemistry - methods Biological and medical sciences Colorimetry - methods Evaluation Studies as Topic Free radical Fundamental and applied biological sciences. Psychology General aspects, methods Hydrochloric Acid - chemistry Indoles - pharmacology Lipid Peroxidation Malondialdehyde - analysis Malondialdehyde - chemistry Malondialdehyde - metabolism Mesylates - chemistry Oxidative Stress Plant physiology and development Plant Proteins - chemistry Plant Proteins - metabolism Ribes - metabolism Spectrophotometry
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