Regulation of dendritic cell differentiation and subset distribution by the zinc finger protein CTCF
Abstract The molecular mechanisms that regulate DC differentiation and subset distribution are largely unknown. In this study we report the identification of the C2 H2 zinc finger transcription factors (TF) CTCF as a regulator of DC differentiation. CTCF was expressed in human and murine DC and its...
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| Veröffentlicht in: | Immunology Letters 2007-04, Vol.109 (2), p.165-174 |
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| container_title | Immunology Letters |
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| creator | Koesters, Christina Unger, Bernd Bilic, Ivan Schmidt, Uwe Bluml, Stefan Lichtenberger, Beate Schreiber, Martin Stockl, Johannes Ellmeier, Wilfried |
| description | Abstract The molecular mechanisms that regulate DC differentiation and subset distribution are largely unknown. In this study we report the identification of the C2 H2 zinc finger transcription factors (TF) CTCF as a regulator of DC differentiation. CTCF was expressed in human and murine DC and its expression was downregulated during the differentiation of human monocyte-derived DC. Enforced expression of CTCF during the differentiation of murine BM-derived DC (BMDC) caused increased apoptosis and reduced proliferation leading to a dramatically reduced number of CTCF transduced DC. The CTCF expressing BMDC that developed had a more immature phenotype than control cells, and showed defects in maturation upon TLR stimulation. Furthermore, in vivo expression of CTCF led to an increase in the percentage of plasmacytoid DC (pDC) within the DC lineage. Our data provide new insight into molecular mechanisms regulating DC differentiation and subset development and identify CTCF as a factor involved in the regulation of these important processes. |
| doi_str_mv | 10.1016/j.imlet.2007.02.006 |
| format | Article |
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In this study we report the identification of the C2 H2 zinc finger transcription factors (TF) CTCF as a regulator of DC differentiation. CTCF was expressed in human and murine DC and its expression was downregulated during the differentiation of human monocyte-derived DC. Enforced expression of CTCF during the differentiation of murine BM-derived DC (BMDC) caused increased apoptosis and reduced proliferation leading to a dramatically reduced number of CTCF transduced DC. The CTCF expressing BMDC that developed had a more immature phenotype than control cells, and showed defects in maturation upon TLR stimulation. Furthermore, in vivo expression of CTCF led to an increase in the percentage of plasmacytoid DC (pDC) within the DC lineage. Our data provide new insight into molecular mechanisms regulating DC differentiation and subset development and identify CTCF as a factor involved in the regulation of these important processes.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.imlet.2007.02.006</identifier><identifier>PMID: 17368809</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergy and Immunology ; Animals ; Bone Marrow Cells - cytology ; Bone Marrow Cells - immunology ; CCCTC-Binding Factor ; Cell differentiation ; Cell Differentiation - immunology ; Cell Growth Processes - immunology ; Cell Survival - immunology ; Dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - immunology ; Down-Regulation ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Repressor Proteins - biosynthesis ; Repressor Proteins - immunology ; Toll-Like Receptors - immunology ; Toll-Like Receptors - metabolism ; Transcription factors ; Transcription Factors - genetics ; Zinc Fingers</subject><ispartof>Immunology Letters, 2007-04, Vol.109 (2), p.165-174</ispartof><rights>Elsevier B.V.</rights><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-9aea252c808fda372a2097eb988a68808e3aa5da9d97bd62463a915f5adef163</citedby><cites>FETCH-LOGICAL-c443t-9aea252c808fda372a2097eb988a68808e3aa5da9d97bd62463a915f5adef163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.imlet.2007.02.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17368809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koesters, Christina</creatorcontrib><creatorcontrib>Unger, Bernd</creatorcontrib><creatorcontrib>Bilic, Ivan</creatorcontrib><creatorcontrib>Schmidt, Uwe</creatorcontrib><creatorcontrib>Bluml, Stefan</creatorcontrib><creatorcontrib>Lichtenberger, Beate</creatorcontrib><creatorcontrib>Schreiber, Martin</creatorcontrib><creatorcontrib>Stockl, Johannes</creatorcontrib><creatorcontrib>Ellmeier, Wilfried</creatorcontrib><title>Regulation of dendritic cell differentiation and subset distribution by the zinc finger protein CTCF</title><title>Immunology Letters</title><addtitle>Immunol Lett</addtitle><description>Abstract The molecular mechanisms that regulate DC differentiation and subset distribution are largely unknown. In this study we report the identification of the C2 H2 zinc finger transcription factors (TF) CTCF as a regulator of DC differentiation. CTCF was expressed in human and murine DC and its expression was downregulated during the differentiation of human monocyte-derived DC. Enforced expression of CTCF during the differentiation of murine BM-derived DC (BMDC) caused increased apoptosis and reduced proliferation leading to a dramatically reduced number of CTCF transduced DC. The CTCF expressing BMDC that developed had a more immature phenotype than control cells, and showed defects in maturation upon TLR stimulation. Furthermore, in vivo expression of CTCF led to an increase in the percentage of plasmacytoid DC (pDC) within the DC lineage. Our data provide new insight into molecular mechanisms regulating DC differentiation and subset development and identify CTCF as a factor involved in the regulation of these important processes.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - immunology</subject><subject>CCCTC-Binding Factor</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - immunology</subject><subject>Cell Growth Processes - immunology</subject><subject>Cell Survival - immunology</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - metabolism</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - immunology</subject><subject>Down-Regulation</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Repressor Proteins - immunology</subject><subject>Toll-Like Receptors - immunology</subject><subject>Toll-Like Receptors - metabolism</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Zinc Fingers</subject><issn>0165-2478</issn><issn>1879-0542</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkGLFDEQhYMo7uzqLxAkJ2_dVpLuTuegIIPrCguCzj2kk-o1Y096TdIL4683vTMgeNlTIPleVeq9IuQNg5oB697va3-YMNccQNbAa4DuGdmwXqoK2oY_J5tCtRVvZH9BLlPaA7BWNOIluWBSdH0PakPcd7xbJpP9HOg8UofBRZ-9pRaniTo_jhgxZH8iTHA0LUPCXJ5Sjn5YHu-HI80_kf7xwdLRhzuM9D7OGX2g2932-hV5MZop4evzeUV2159325vq9tuXr9tPt5VtGpErZdDwltse-tEZIbnhoCQOqu_N-t0ehTGtM8opObiON50wirVjaxyOrBNX5N2pbOn9e8GU9cGndQ4TcF6SliAYALRPgkx1UrUcCihOoI1zShFHfR_9wcSjZqDXEPReP4ag1xA0cF1CKKq35_LLcED3T3N2vQAfTgAWMx48Rp2sx2DR-Yg2azf7Jxp8_E9vJx-8NdMvPGLaz0sMxWfNdCoC_WPdg3UNQJbxRcvEXweTryo</recordid><startdate>20070415</startdate><enddate>20070415</enddate><creator>Koesters, Christina</creator><creator>Unger, Bernd</creator><creator>Bilic, Ivan</creator><creator>Schmidt, Uwe</creator><creator>Bluml, Stefan</creator><creator>Lichtenberger, Beate</creator><creator>Schreiber, Martin</creator><creator>Stockl, Johannes</creator><creator>Ellmeier, Wilfried</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20070415</creationdate><title>Regulation of dendritic cell differentiation and subset distribution by the zinc finger protein CTCF</title><author>Koesters, Christina ; Unger, Bernd ; Bilic, Ivan ; Schmidt, Uwe ; Bluml, Stefan ; Lichtenberger, Beate ; Schreiber, Martin ; Stockl, Johannes ; Ellmeier, Wilfried</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-9aea252c808fda372a2097eb988a68808e3aa5da9d97bd62463a915f5adef163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - immunology</topic><topic>CCCTC-Binding Factor</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - immunology</topic><topic>Cell Growth Processes - immunology</topic><topic>Cell Survival - immunology</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - metabolism</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - immunology</topic><topic>Down-Regulation</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Repressor Proteins - immunology</topic><topic>Toll-Like Receptors - immunology</topic><topic>Toll-Like Receptors - metabolism</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koesters, Christina</creatorcontrib><creatorcontrib>Unger, Bernd</creatorcontrib><creatorcontrib>Bilic, Ivan</creatorcontrib><creatorcontrib>Schmidt, Uwe</creatorcontrib><creatorcontrib>Bluml, Stefan</creatorcontrib><creatorcontrib>Lichtenberger, Beate</creatorcontrib><creatorcontrib>Schreiber, Martin</creatorcontrib><creatorcontrib>Stockl, Johannes</creatorcontrib><creatorcontrib>Ellmeier, Wilfried</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology Letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koesters, Christina</au><au>Unger, Bernd</au><au>Bilic, Ivan</au><au>Schmidt, Uwe</au><au>Bluml, Stefan</au><au>Lichtenberger, Beate</au><au>Schreiber, Martin</au><au>Stockl, Johannes</au><au>Ellmeier, Wilfried</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of dendritic cell differentiation and subset distribution by the zinc finger protein CTCF</atitle><jtitle>Immunology Letters</jtitle><addtitle>Immunol Lett</addtitle><date>2007-04-15</date><risdate>2007</risdate><volume>109</volume><issue>2</issue><spage>165</spage><epage>174</epage><pages>165-174</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><eissn>1365-2567</eissn><abstract>Abstract The molecular mechanisms that regulate DC differentiation and subset distribution are largely unknown. In this study we report the identification of the C2 H2 zinc finger transcription factors (TF) CTCF as a regulator of DC differentiation. CTCF was expressed in human and murine DC and its expression was downregulated during the differentiation of human monocyte-derived DC. Enforced expression of CTCF during the differentiation of murine BM-derived DC (BMDC) caused increased apoptosis and reduced proliferation leading to a dramatically reduced number of CTCF transduced DC. The CTCF expressing BMDC that developed had a more immature phenotype than control cells, and showed defects in maturation upon TLR stimulation. Furthermore, in vivo expression of CTCF led to an increase in the percentage of plasmacytoid DC (pDC) within the DC lineage. Our data provide new insight into molecular mechanisms regulating DC differentiation and subset development and identify CTCF as a factor involved in the regulation of these important processes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17368809</pmid><doi>10.1016/j.imlet.2007.02.006</doi><tpages>10</tpages></addata></record> |
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| ispartof | Immunology Letters, 2007-04, Vol.109 (2), p.165-174 |
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| language | eng |
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| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; PubMed Central |
| subjects | Allergy and Immunology Animals Bone Marrow Cells - cytology Bone Marrow Cells - immunology CCCTC-Binding Factor Cell differentiation Cell Differentiation - immunology Cell Growth Processes - immunology Cell Survival - immunology Dendritic cells Dendritic Cells - cytology Dendritic Cells - immunology Dendritic Cells - metabolism DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - immunology Down-Regulation Humans Mice Mice, Inbred BALB C Mice, Inbred C57BL Repressor Proteins - biosynthesis Repressor Proteins - immunology Toll-Like Receptors - immunology Toll-Like Receptors - metabolism Transcription factors Transcription Factors - genetics Zinc Fingers |
| title | Regulation of dendritic cell differentiation and subset distribution by the zinc finger protein CTCF |
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