CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic se...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2007-03, Vol.315 (5819), p.1709-1712 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Barrangou, Rodolphe Fremaux, Christophe Deveau, Hélène Richards, Melissa Boyaval, Patrick Moineau, Sylvain Romero, Dennis A Horvath, Philippe |
| description | Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity. |
| doi_str_mv | 10.1126/science.1138140 |
| format | Article |
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We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1138140</identifier><identifier>PMID: 17379808</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Archaea ; Bacteria ; Bacteriology ; Bacteriophages ; Biological and medical sciences ; DNA, Bacterial - genetics ; DNA, Intergenic - genetics ; Evolution ; Evolution, Molecular ; Fundamental and applied biological sciences. 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We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.</description><subject>Archaea</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Bacteriophages</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Intergenic - genetics</subject><subject>Evolution</subject><subject>Evolution, Molecular</subject><subject>Fundamental and applied biological sciences. 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We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>17379808</pmid><doi>10.1126/science.1138140</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Archaea Bacteria Bacteriology Bacteriophages Biological and medical sciences DNA, Bacterial - genetics DNA, Intergenic - genetics Evolution Evolution, Molecular Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Genetic loci Genetics Genome, Viral Genomes Genomics Genotype & phenotype Immunity Microbiology Molecular Sequence Data Mutation Open reading frames Polymorphism, Single Nucleotide Prokaryotes Repetitive Sequences, Nucleic Acid Streptococcus Phages - genetics Streptococcus Phages - physiology Streptococcus thermophilus - genetics Streptococcus thermophilus - virology Viral Plaque Assay Virus Replication Viruses |
| title | CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes |
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