Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models

There is a growing demand for a cost-effective, efficient, and high-throughput method for measuring cytokines. Currently, many studies are using flow cytometric bead-based multiplex assays in the measurement of cytokines. However, limited data are available regarding the performance of these cytomet...

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Veröffentlicht in:	Journal of immunological methods 2008-02, Vol.331 (1), p.59-68
Hauptverfasser:	Young, Shih-Houng, Antonini, James M., Roberts, Jenny R., Erdely, Aaron D., Zeidler-Erdely, Patti C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Bronchoalveolar Lavage Fluid - chemistry Cytokines Cytokines - analysis Cytokines - immunology ELISA Enzyme-Linked Immunosorbent Assay - methods Flow cytometric bead assays Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Lung - chemistry Male Mice Mice, Inbred C57BL Molecular immunology Multiplex immunoassay Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger RT-PCR Sensitivity and Specificity Techniques Zymosan - immunology
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