Intramyocardial injection of vascular endothelial growth factor gene improves cardiac performance and inhibits cardiomyocyte apoptosis
Background: Previous studies suggest that vascular endothelial growth factor (VEGF) is a regulator of naturally occurring angiogenesis. However, whether VEGF plays a role in cardiomyocyte apoptosis is not known. Aim: To investigate the effects of intramyocardial injection of VEGF165 cDNA on cardiac...
Gespeichert in:
| Veröffentlicht in: | European journal of heart failure 2007-04, Vol.9 (4), p.343-351 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 351 |
|---|---|
| container_issue | 4 |
| container_start_page | 343 |
| container_title | European journal of heart failure |
| container_volume | 9 |
| creator | Ruixing, Yin Dezhai, Yang Hai, Wu Kai, Huang Xianghong, Wu Yuming, Chen |
| description | Background:
Previous studies suggest that vascular endothelial growth factor (VEGF) is a regulator of naturally occurring angiogenesis. However, whether VEGF plays a role in cardiomyocyte apoptosis is not known.
Aim:
To investigate the effects of intramyocardial injection of VEGF165 cDNA on cardiac performance and cardiomyocyte apoptosis in a rat model of acute myocardial infarction.
Methods:
Forty male Sprague-Dawley rats underwent left coronary artery ligation and were randomised to receive VEGF165 cDNA (treated group) or pcDNA3.1 (control), injected directly into the border zone of the ischaemic myocardium. Twenty rats underwent thoracotomy and injection of pcDNA3.1, without coronary ligation (sham group). Haemodynamic and apoptotic parameters were measured two weeks after injection.
Results:
Three sham, eight control, and five treated animals died. Haemodynamic parameters and microvessel counts in the treated group were significantly better than in the control (P |
| doi_str_mv | 10.1016/j.ejheart.2006.10.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70303718</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1016/j.ejheart.2006.10.007</oup_id><sourcerecordid>70303718</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5117-db7dc627540470f558259ca3b53ae38c2b5eea60d1e901f4902aada9755c52f33</originalsourceid><addsrcrecordid>eNqNkcFu1DAURSNERUvhE0BescvwHMdxsoRR22nVgpCALi3Heek4JHFqOy3zA_1uEiUCsevK1vO59z75RtE7ChsKNPvYbLDZo3JhkwBk02wDIF5EJzQXRQx5mr6c7izP4yJPk-PotfcNABUAyavomIqEU-DFSfR02QenuoPVylVGtcT0DepgbE9sTR6U12OrHMG-smGP7UzcOfsY9qRWOlhH7rBHYrrB2Qf0ZHHRZEBXW9epXiNRfTW57k1pwgrYOe8QpqfBDsF6499ER7VqPb5dz9Pox_nZ9-0uvv56cbn9dB1rTqmIq1JUOksETyEVUHOeJ7zQipWcKWS5TkqOqDKoKBZA67SARKlKFYJzzZOasdPow-I77Xs_og-yM15j26oe7eilAAZM0HwC-QJqZ713WMvBmU65g6Qg5wJkI9cC5FzAPJ4KmHTv14Cx7LD6p1p_fAI-L8CjafHwPFd5drU7_z8FFhM7Ds9eLF4kxgf8_Vek3C-ZCSa4vP1yIW9S-m1Ld7fyJ_sDLau7KQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70303718</pqid></control><display><type>article</type><title>Intramyocardial injection of vascular endothelial growth factor gene improves cardiac performance and inhibits cardiomyocyte apoptosis</title><source>MEDLINE</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><creator>Ruixing, Yin ; Dezhai, Yang ; Hai, Wu ; Kai, Huang ; Xianghong, Wu ; Yuming, Chen</creator><creatorcontrib>Ruixing, Yin ; Dezhai, Yang ; Hai, Wu ; Kai, Huang ; Xianghong, Wu ; Yuming, Chen</creatorcontrib><description>Background:
Previous studies suggest that vascular endothelial growth factor (VEGF) is a regulator of naturally occurring angiogenesis. However, whether VEGF plays a role in cardiomyocyte apoptosis is not known.
Aim:
To investigate the effects of intramyocardial injection of VEGF165 cDNA on cardiac performance and cardiomyocyte apoptosis in a rat model of acute myocardial infarction.
Methods:
Forty male Sprague-Dawley rats underwent left coronary artery ligation and were randomised to receive VEGF165 cDNA (treated group) or pcDNA3.1 (control), injected directly into the border zone of the ischaemic myocardium. Twenty rats underwent thoracotomy and injection of pcDNA3.1, without coronary ligation (sham group). Haemodynamic and apoptotic parameters were measured two weeks after injection.
Results:
Three sham, eight control, and five treated animals died. Haemodynamic parameters and microvessel counts in the treated group were significantly better than in the control (P<0.05 to 0.01). Apoptotic index in the treated group was less than in the control (P<0.01). Caspase-3 activation and mitochondrial cytochrome c release in the treated group were also lower than in the control (P<0.01). VEGF165 cDNA treatment significantly inhibited p53, Fas, Bax, and increased VEGF and Bcl-2 expression in the myocardium.
Conclusion:
Intramyocardial injection of VEGF165 cDNA significantly improved cardiac performance, stimulated angiogenesis and reduced cardiomyocyte apoptosis, in a rat model of acute myocardial infarction.</description><identifier>ISSN: 1388-9842</identifier><identifier>EISSN: 1879-0844</identifier><identifier>DOI: 10.1016/j.ejheart.2006.10.007</identifier><identifier>PMID: 17251059</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>angiogenesis ; Animals ; Apoptosis ; DNA, Complementary ; Gene Expression ; gene therapy ; Genetic Therapy ; heart failure ; Heart Failure - etiology ; Heart Failure - physiopathology ; Immunohistochemistry ; Male ; Muscle Cells ; Myocardial Infarction ; Myocardial Ischemia ; Myocardium - cytology ; Myocardium - metabolism ; Rats ; Rats, Sprague-Dawley ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; vascular endothelial growth factors</subject><ispartof>European journal of heart failure, 2007-04, Vol.9 (4), p.343-351</ispartof><rights>2007 European Society of Cardiology 2007</rights><rights>Published on behalf of the European Society of Cardiology. All rights reserved. © 2007 the Authors</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5117-db7dc627540470f558259ca3b53ae38c2b5eea60d1e901f4902aada9755c52f33</citedby><cites>FETCH-LOGICAL-c5117-db7dc627540470f558259ca3b53ae38c2b5eea60d1e901f4902aada9755c52f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.ejheart.2006.10.007$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.ejheart.2006.10.007$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17251059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruixing, Yin</creatorcontrib><creatorcontrib>Dezhai, Yang</creatorcontrib><creatorcontrib>Hai, Wu</creatorcontrib><creatorcontrib>Kai, Huang</creatorcontrib><creatorcontrib>Xianghong, Wu</creatorcontrib><creatorcontrib>Yuming, Chen</creatorcontrib><title>Intramyocardial injection of vascular endothelial growth factor gene improves cardiac performance and inhibits cardiomyocyte apoptosis</title><title>European journal of heart failure</title><addtitle>European Journal of Heart Failure</addtitle><description>Background:
Previous studies suggest that vascular endothelial growth factor (VEGF) is a regulator of naturally occurring angiogenesis. However, whether VEGF plays a role in cardiomyocyte apoptosis is not known.
Aim:
To investigate the effects of intramyocardial injection of VEGF165 cDNA on cardiac performance and cardiomyocyte apoptosis in a rat model of acute myocardial infarction.
Methods:
Forty male Sprague-Dawley rats underwent left coronary artery ligation and were randomised to receive VEGF165 cDNA (treated group) or pcDNA3.1 (control), injected directly into the border zone of the ischaemic myocardium. Twenty rats underwent thoracotomy and injection of pcDNA3.1, without coronary ligation (sham group). Haemodynamic and apoptotic parameters were measured two weeks after injection.
Results:
Three sham, eight control, and five treated animals died. Haemodynamic parameters and microvessel counts in the treated group were significantly better than in the control (P<0.05 to 0.01). Apoptotic index in the treated group was less than in the control (P<0.01). Caspase-3 activation and mitochondrial cytochrome c release in the treated group were also lower than in the control (P<0.01). VEGF165 cDNA treatment significantly inhibited p53, Fas, Bax, and increased VEGF and Bcl-2 expression in the myocardium.
Conclusion:
Intramyocardial injection of VEGF165 cDNA significantly improved cardiac performance, stimulated angiogenesis and reduced cardiomyocyte apoptosis, in a rat model of acute myocardial infarction.</description><subject>angiogenesis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>DNA, Complementary</subject><subject>Gene Expression</subject><subject>gene therapy</subject><subject>Genetic Therapy</subject><subject>heart failure</subject><subject>Heart Failure - etiology</subject><subject>Heart Failure - physiopathology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Muscle Cells</subject><subject>Myocardial Infarction</subject><subject>Myocardial Ischemia</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>vascular endothelial growth factors</subject><issn>1388-9842</issn><issn>1879-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAURSNERUvhE0BescvwHMdxsoRR22nVgpCALi3Heek4JHFqOy3zA_1uEiUCsevK1vO59z75RtE7ChsKNPvYbLDZo3JhkwBk02wDIF5EJzQXRQx5mr6c7izP4yJPk-PotfcNABUAyavomIqEU-DFSfR02QenuoPVylVGtcT0DepgbE9sTR6U12OrHMG-smGP7UzcOfsY9qRWOlhH7rBHYrrB2Qf0ZHHRZEBXW9epXiNRfTW57k1pwgrYOe8QpqfBDsF6499ER7VqPb5dz9Pox_nZ9-0uvv56cbn9dB1rTqmIq1JUOksETyEVUHOeJ7zQipWcKWS5TkqOqDKoKBZA67SARKlKFYJzzZOasdPow-I77Xs_og-yM15j26oe7eilAAZM0HwC-QJqZ713WMvBmU65g6Qg5wJkI9cC5FzAPJ4KmHTv14Cx7LD6p1p_fAI-L8CjafHwPFd5drU7_z8FFhM7Ds9eLF4kxgf8_Vek3C-ZCSa4vP1yIW9S-m1Ld7fyJ_sDLau7KQ</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Ruixing, Yin</creator><creator>Dezhai, Yang</creator><creator>Hai, Wu</creator><creator>Kai, Huang</creator><creator>Xianghong, Wu</creator><creator>Yuming, Chen</creator><general>Blackwell Publishing Ltd</general><general>Elsevier</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>Intramyocardial injection of vascular endothelial growth factor gene improves cardiac performance and inhibits cardiomyocyte apoptosis</title><author>Ruixing, Yin ; Dezhai, Yang ; Hai, Wu ; Kai, Huang ; Xianghong, Wu ; Yuming, Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5117-db7dc627540470f558259ca3b53ae38c2b5eea60d1e901f4902aada9755c52f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>angiogenesis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>DNA, Complementary</topic><topic>Gene Expression</topic><topic>gene therapy</topic><topic>Genetic Therapy</topic><topic>heart failure</topic><topic>Heart Failure - etiology</topic><topic>Heart Failure - physiopathology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Muscle Cells</topic><topic>Myocardial Infarction</topic><topic>Myocardial Ischemia</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>vascular endothelial growth factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruixing, Yin</creatorcontrib><creatorcontrib>Dezhai, Yang</creatorcontrib><creatorcontrib>Hai, Wu</creatorcontrib><creatorcontrib>Kai, Huang</creatorcontrib><creatorcontrib>Xianghong, Wu</creatorcontrib><creatorcontrib>Yuming, Chen</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of heart failure</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruixing, Yin</au><au>Dezhai, Yang</au><au>Hai, Wu</au><au>Kai, Huang</au><au>Xianghong, Wu</au><au>Yuming, Chen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intramyocardial injection of vascular endothelial growth factor gene improves cardiac performance and inhibits cardiomyocyte apoptosis</atitle><jtitle>European journal of heart failure</jtitle><addtitle>European Journal of Heart Failure</addtitle><date>2007-04</date><risdate>2007</risdate><volume>9</volume><issue>4</issue><spage>343</spage><epage>351</epage><pages>343-351</pages><issn>1388-9842</issn><eissn>1879-0844</eissn><abstract>Background:
Previous studies suggest that vascular endothelial growth factor (VEGF) is a regulator of naturally occurring angiogenesis. However, whether VEGF plays a role in cardiomyocyte apoptosis is not known.
Aim:
To investigate the effects of intramyocardial injection of VEGF165 cDNA on cardiac performance and cardiomyocyte apoptosis in a rat model of acute myocardial infarction.
Methods:
Forty male Sprague-Dawley rats underwent left coronary artery ligation and were randomised to receive VEGF165 cDNA (treated group) or pcDNA3.1 (control), injected directly into the border zone of the ischaemic myocardium. Twenty rats underwent thoracotomy and injection of pcDNA3.1, without coronary ligation (sham group). Haemodynamic and apoptotic parameters were measured two weeks after injection.
Results:
Three sham, eight control, and five treated animals died. Haemodynamic parameters and microvessel counts in the treated group were significantly better than in the control (P<0.05 to 0.01). Apoptotic index in the treated group was less than in the control (P<0.01). Caspase-3 activation and mitochondrial cytochrome c release in the treated group were also lower than in the control (P<0.01). VEGF165 cDNA treatment significantly inhibited p53, Fas, Bax, and increased VEGF and Bcl-2 expression in the myocardium.
Conclusion:
Intramyocardial injection of VEGF165 cDNA significantly improved cardiac performance, stimulated angiogenesis and reduced cardiomyocyte apoptosis, in a rat model of acute myocardial infarction.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>17251059</pmid><doi>10.1016/j.ejheart.2006.10.007</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1388-9842 |
| ispartof | European journal of heart failure, 2007-04, Vol.9 (4), p.343-351 |
| issn | 1388-9842 1879-0844 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70303718 |
| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals |
| subjects | angiogenesis Animals Apoptosis DNA, Complementary Gene Expression gene therapy Genetic Therapy heart failure Heart Failure - etiology Heart Failure - physiopathology Immunohistochemistry Male Muscle Cells Myocardial Infarction Myocardial Ischemia Myocardium - cytology Myocardium - metabolism Rats Rats, Sprague-Dawley Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism vascular endothelial growth factors |
| title | Intramyocardial injection of vascular endothelial growth factor gene improves cardiac performance and inhibits cardiomyocyte apoptosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T20%3A35%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intramyocardial%20injection%20of%20vascular%20endothelial%20growth%20factor%20gene%20improves%20cardiac%20performance%20and%20inhibits%20cardiomyocyte%20apoptosis&rft.jtitle=European%20journal%20of%20heart%20failure&rft.au=Ruixing,%20Yin&rft.date=2007-04&rft.volume=9&rft.issue=4&rft.spage=343&rft.epage=351&rft.pages=343-351&rft.issn=1388-9842&rft.eissn=1879-0844&rft_id=info:doi/10.1016/j.ejheart.2006.10.007&rft_dat=%3Cproquest_cross%3E70303718%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70303718&rft_id=info:pmid/17251059&rft_oup_id=10.1016/j.ejheart.2006.10.007&rfr_iscdi=true |