Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein
Summary Johne's disease (JD) is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Here, we report the cloning and expression of a 74 kDa recombinant polyprotein (Map74F) and its protective efficacy against MAP infection in mice. Map74F was ge...
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| Veröffentlicht in: | Vaccine 2008-02, Vol.26 (9), p.1253-1262 |
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| creator | Chen, Li-Hsuen Kathaperumal, Kumanan Huang, Ching-Juo McDonough, Sean P Stehman, Susan Akey, Bruce Huntley, John Bannantine, John P Chang, Chao-Fu Chang, Yung-Fu |
| description | Summary Johne's disease (JD) is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Here, we report the cloning and expression of a 74 kDa recombinant polyprotein (Map74F) and its protective efficacy against MAP infection in mice. Map74F was generated by the sequential linkage of the ORFs of the ∼17.6-kDa C-terminal fragment of Map3527 to the full-length ORF of Map1519, followed at the C-terminus with ∼14.6-kDa N-terminal portion of Map3527. Mice immunized with Map74F had a significant IgG1 response but not IgG2a. In immunized animals, the IgG1/IgG2a ratio increased until 4 weeks after MAP challenge. The ratio decreased from 8 weeks indicating a shift to a Th1 response. Antigen specific IFN-γ response, CD3+ and CD4+ T cells increased significantly in immunized mice. Following challenge, MAP burden was significantly lower in liver, spleen and mesenteric lymph nodes of immunized animals compared to control animals indicating protection against MAP infection. This was further evident by the improved liver and spleen pathology of the immunized animals, which had fewer granulomas and lower numbers of acid-fast bacilli. Results of this study indicated that immunization of mice with Map74F protected mice against MAP infection. |
| doi_str_mv | 10.1016/j.vaccine.2007.12.014 |
| format | Article |
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Here, we report the cloning and expression of a 74 kDa recombinant polyprotein (Map74F) and its protective efficacy against MAP infection in mice. Map74F was generated by the sequential linkage of the ORFs of the ∼17.6-kDa C-terminal fragment of Map3527 to the full-length ORF of Map1519, followed at the C-terminus with ∼14.6-kDa N-terminal portion of Map3527. Mice immunized with Map74F had a significant IgG1 response but not IgG2a. In immunized animals, the IgG1/IgG2a ratio increased until 4 weeks after MAP challenge. The ratio decreased from 8 weeks indicating a shift to a Th1 response. Antigen specific IFN-γ response, CD3+ and CD4+ T cells increased significantly in immunized mice. Following challenge, MAP burden was significantly lower in liver, spleen and mesenteric lymph nodes of immunized animals compared to control animals indicating protection against MAP infection. This was further evident by the improved liver and spleen pathology of the immunized animals, which had fewer granulomas and lower numbers of acid-fast bacilli. Results of this study indicated that immunization of mice with Map74F protected mice against MAP infection.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2007.12.014</identifier><identifier>PMID: 18243427</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animals ; Antibodies, Bacterial - blood ; Bacterial Vaccines - administration & dosage ; Bacterial Vaccines - immunology ; Cattle ; CD3 Complex - metabolism ; CD4 Antigens - metabolism ; Female ; Immunization ; Immunoglobulin G - blood ; Interferon-gamma - biosynthesis ; Johne's disease ; Mice ; Mice, Inbred C57BL ; Mycobacterium avium ; Mycobacterium avium subsp. paratuberculosis - immunology ; Paratuberculosis ; Paratuberculosis - immunology ; Paratuberculosis - microbiology ; Paratuberculosis - prevention & control ; Polyproteins ; Polyproteins - administration & dosage ; Polyproteins - chemistry ; Polyproteins - genetics ; Polyproteins - immunology ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Ruminantia ; T-Lymphocytes - immunology ; Vaccination ; Vaccine trials</subject><ispartof>Vaccine, 2008-02, Vol.26 (9), p.1253-1262</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-3bc3efbdb6205473e370421a06d9a7b90859957f640d932d269a3c9ea502c993</citedby><cites>FETCH-LOGICAL-c449t-3bc3efbdb6205473e370421a06d9a7b90859957f640d932d269a3c9ea502c993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X07014788$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18243427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Li-Hsuen</creatorcontrib><creatorcontrib>Kathaperumal, Kumanan</creatorcontrib><creatorcontrib>Huang, Ching-Juo</creatorcontrib><creatorcontrib>McDonough, Sean P</creatorcontrib><creatorcontrib>Stehman, Susan</creatorcontrib><creatorcontrib>Akey, Bruce</creatorcontrib><creatorcontrib>Huntley, John</creatorcontrib><creatorcontrib>Bannantine, John P</creatorcontrib><creatorcontrib>Chang, Chao-Fu</creatorcontrib><creatorcontrib>Chang, Yung-Fu</creatorcontrib><title>Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Summary Johne's disease (JD) is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Here, we report the cloning and expression of a 74 kDa recombinant polyprotein (Map74F) and its protective efficacy against MAP infection in mice. Map74F was generated by the sequential linkage of the ORFs of the ∼17.6-kDa C-terminal fragment of Map3527 to the full-length ORF of Map1519, followed at the C-terminus with ∼14.6-kDa N-terminal portion of Map3527. Mice immunized with Map74F had a significant IgG1 response but not IgG2a. In immunized animals, the IgG1/IgG2a ratio increased until 4 weeks after MAP challenge. The ratio decreased from 8 weeks indicating a shift to a Th1 response. Antigen specific IFN-γ response, CD3+ and CD4+ T cells increased significantly in immunized mice. Following challenge, MAP burden was significantly lower in liver, spleen and mesenteric lymph nodes of immunized animals compared to control animals indicating protection against MAP infection. This was further evident by the improved liver and spleen pathology of the immunized animals, which had fewer granulomas and lower numbers of acid-fast bacilli. Results of this study indicated that immunization of mice with Map74F protected mice against MAP infection.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacterial Vaccines - administration & dosage</subject><subject>Bacterial Vaccines - immunology</subject><subject>Cattle</subject><subject>CD3 Complex - metabolism</subject><subject>CD4 Antigens - metabolism</subject><subject>Female</subject><subject>Immunization</subject><subject>Immunoglobulin G - blood</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Johne's disease</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium subsp. paratuberculosis - immunology</subject><subject>Paratuberculosis</subject><subject>Paratuberculosis - immunology</subject><subject>Paratuberculosis - microbiology</subject><subject>Paratuberculosis - prevention & control</subject><subject>Polyproteins</subject><subject>Polyproteins - administration & dosage</subject><subject>Polyproteins - chemistry</subject><subject>Polyproteins - genetics</subject><subject>Polyproteins - immunology</subject><subject>Recombinant Proteins - administration & dosage</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Ruminantia</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccination</subject><subject>Vaccine trials</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhSMEokPhEUBesUu4_kkcb0CoaqFSEQu6YGc5zg14SOxgJ1PNC_DcOJqRkNh0Yy_8nXusc25RvKZQUaDNu311MNY6jxUDkBVlFVDxpNjRVvKS1bR9WuyANaIUFL5fFC9S2gNAzal6XlzQlgkumNwVf26nafVIIqY5-ISJOE8mZ5EsgXw52tAZu2B060TMYTvT2qW5IrOJZlk7jHYdQ3KJDGEcw4PzP8jpX2ZxwZMHt_wkhvhwwJFIcZN9bJi6_OwXMofxOMewoPMvi2eDGRO-Ot-Xxf3N9f3V5_Lu66fbq493pRVCLSXvLMeh67uGQS0kRy5BMGqg6ZWRnYK2VqqWQyOgV5z1rFGGW4WmBmaV4pfF29PYbPt7xbToySWL42g8hjVpCRxYnRN8DGQUatk2G1ifQBtDShEHPUc3mXjUFPRWlN7rc1F6K0pTpnNRWffmbLB2E_b_VOdmMvDhBGCO4-Aw6mQdeou9yxkuug_uUYv3_02wo_POmvEXHjHtwxp9zlpTnbJAf9u2ZVsWkFkt25b_BSkJvkY</recordid><startdate>20080226</startdate><enddate>20080226</enddate><creator>Chen, Li-Hsuen</creator><creator>Kathaperumal, Kumanan</creator><creator>Huang, Ching-Juo</creator><creator>McDonough, Sean P</creator><creator>Stehman, Susan</creator><creator>Akey, Bruce</creator><creator>Huntley, John</creator><creator>Bannantine, John P</creator><creator>Chang, Chao-Fu</creator><creator>Chang, Yung-Fu</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080226</creationdate><title>Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein</title><author>Chen, Li-Hsuen ; Kathaperumal, Kumanan ; Huang, Ching-Juo ; McDonough, Sean P ; Stehman, Susan ; Akey, Bruce ; Huntley, John ; Bannantine, John P ; Chang, Chao-Fu ; Chang, Yung-Fu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-3bc3efbdb6205473e370421a06d9a7b90859957f640d932d269a3c9ea502c993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bacterial Vaccines - administration & dosage</topic><topic>Bacterial Vaccines - immunology</topic><topic>Cattle</topic><topic>CD3 Complex - metabolism</topic><topic>CD4 Antigens - metabolism</topic><topic>Female</topic><topic>Immunization</topic><topic>Immunoglobulin G - blood</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Johne's disease</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium subsp. paratuberculosis - immunology</topic><topic>Paratuberculosis</topic><topic>Paratuberculosis - immunology</topic><topic>Paratuberculosis - microbiology</topic><topic>Paratuberculosis - prevention & control</topic><topic>Polyproteins</topic><topic>Polyproteins - administration & dosage</topic><topic>Polyproteins - chemistry</topic><topic>Polyproteins - genetics</topic><topic>Polyproteins - immunology</topic><topic>Recombinant Proteins - administration & dosage</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Ruminantia</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccination</topic><topic>Vaccine trials</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Li-Hsuen</creatorcontrib><creatorcontrib>Kathaperumal, Kumanan</creatorcontrib><creatorcontrib>Huang, Ching-Juo</creatorcontrib><creatorcontrib>McDonough, Sean P</creatorcontrib><creatorcontrib>Stehman, Susan</creatorcontrib><creatorcontrib>Akey, Bruce</creatorcontrib><creatorcontrib>Huntley, John</creatorcontrib><creatorcontrib>Bannantine, John P</creatorcontrib><creatorcontrib>Chang, Chao-Fu</creatorcontrib><creatorcontrib>Chang, Yung-Fu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Li-Hsuen</au><au>Kathaperumal, Kumanan</au><au>Huang, Ching-Juo</au><au>McDonough, Sean P</au><au>Stehman, Susan</au><au>Akey, Bruce</au><au>Huntley, John</au><au>Bannantine, John P</au><au>Chang, Chao-Fu</au><au>Chang, Yung-Fu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2008-02-26</date><risdate>2008</risdate><volume>26</volume><issue>9</issue><spage>1253</spage><epage>1262</epage><pages>1253-1262</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Summary Johne's disease (JD) is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Here, we report the cloning and expression of a 74 kDa recombinant polyprotein (Map74F) and its protective efficacy against MAP infection in mice. Map74F was generated by the sequential linkage of the ORFs of the ∼17.6-kDa C-terminal fragment of Map3527 to the full-length ORF of Map1519, followed at the C-terminus with ∼14.6-kDa N-terminal portion of Map3527. Mice immunized with Map74F had a significant IgG1 response but not IgG2a. In immunized animals, the IgG1/IgG2a ratio increased until 4 weeks after MAP challenge. The ratio decreased from 8 weeks indicating a shift to a Th1 response. Antigen specific IFN-γ response, CD3+ and CD4+ T cells increased significantly in immunized mice. Following challenge, MAP burden was significantly lower in liver, spleen and mesenteric lymph nodes of immunized animals compared to control animals indicating protection against MAP infection. This was further evident by the improved liver and spleen pathology of the immunized animals, which had fewer granulomas and lower numbers of acid-fast bacilli. Results of this study indicated that immunization of mice with Map74F protected mice against MAP infection.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>18243427</pmid><doi>10.1016/j.vaccine.2007.12.014</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Allergy and Immunology Animals Antibodies, Bacterial - blood Bacterial Vaccines - administration & dosage Bacterial Vaccines - immunology Cattle CD3 Complex - metabolism CD4 Antigens - metabolism Female Immunization Immunoglobulin G - blood Interferon-gamma - biosynthesis Johne's disease Mice Mice, Inbred C57BL Mycobacterium avium Mycobacterium avium subsp. paratuberculosis - immunology Paratuberculosis Paratuberculosis - immunology Paratuberculosis - microbiology Paratuberculosis - prevention & control Polyproteins Polyproteins - administration & dosage Polyproteins - chemistry Polyproteins - genetics Polyproteins - immunology Recombinant Proteins - administration & dosage Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Ruminantia T-Lymphocytes - immunology Vaccination Vaccine trials |
| title | Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein |
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