Influence of cryopreservation on human periodontal ligament cells in vitro
Cryopreservation of teeth before autotransplantation may create new possibilities in dentistry. The purpose of this study was to examine the effect of a standardised cryopreservation procedure on human periodontal ligament (PDL) cell cultures. Human PDL fibroblasts obtained from immature third molar...
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| Veröffentlicht in: | Cell and tissue banking 2008-03, Vol.9 (1), p.11-18 |
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| creator | Temmerman, Liesbeth Dermaut, Luc R. De Mil, Martine Van Maele, Georges Beele, Hilde De Pauw, Guy A. M. |
| description | Cryopreservation of teeth before autotransplantation may create new possibilities in dentistry. The purpose of this study was to examine the effect of a standardised cryopreservation procedure on human periodontal ligament (PDL) cell cultures. Human PDL fibroblasts obtained from immature third molars of 11 patients were cultured and divided into two groups. The experimental group was cryopreserved and cultured after thawing. The control group was cultured without cryopreservation. A comparison was made between cryopreserved and control cells. To evaluate possible differences in the characteristics of the fibroblasts, the cells in both groups were tested for viability (membrane integrity), growth capacity and alkaline phosphatase (ALP) expression. The Wilcoxon test for paired comparison between cryopreserved and non-cryopreserved cells was performed for each characteristic. The results showed that membrane integrity of cells was not influenced by cryopreservation. There was no statistically significant difference in growth capacity between cryopreserved and control cells. Non-cryopreserved cells were slightly stronger positive for ALP, but the difference was not statistically significant. From these experiments it can be concluded that the observed parameters are not influenced by cryopreservation. |
| doi_str_mv | 10.1007/s10561-007-9047-y |
| format | Article |
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M.</creator><creatorcontrib>Temmerman, Liesbeth ; Dermaut, Luc R. ; De Mil, Martine ; Van Maele, Georges ; Beele, Hilde ; De Pauw, Guy A. M.</creatorcontrib><description>Cryopreservation of teeth before autotransplantation may create new possibilities in dentistry. The purpose of this study was to examine the effect of a standardised cryopreservation procedure on human periodontal ligament (PDL) cell cultures. Human PDL fibroblasts obtained from immature third molars of 11 patients were cultured and divided into two groups. The experimental group was cryopreserved and cultured after thawing. The control group was cultured without cryopreservation. A comparison was made between cryopreserved and control cells. To evaluate possible differences in the characteristics of the fibroblasts, the cells in both groups were tested for viability (membrane integrity), growth capacity and alkaline phosphatase (ALP) expression. The Wilcoxon test for paired comparison between cryopreserved and non-cryopreserved cells was performed for each characteristic. The results showed that membrane integrity of cells was not influenced by cryopreservation. There was no statistically significant difference in growth capacity between cryopreserved and control cells. Non-cryopreserved cells were slightly stronger positive for ALP, but the difference was not statistically significant. From these experiments it can be concluded that the observed parameters are not influenced by cryopreservation.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-007-9047-y</identifier><identifier>PMID: 17541731</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adolescent ; Adult ; Alkaline Phosphatase - metabolism ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Cell Biology ; Cell culture ; Cell Membrane - pathology ; Cell Membrane - physiology ; Cell Proliferation ; Cell Survival - physiology ; Cells, Cultured ; Comparative studies ; Cryopreservation - methods ; Cryotherapy ; Fibroblasts - enzymology ; Fibroblasts - pathology ; Fibroblasts - physiology ; Humans ; Life Sciences ; Ligaments ; Medical sciences ; Periodontal Ligament - cytology ; Periodontal Ligament - enzymology ; Periodontal Ligament - physiology ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Teeth ; Temperature effects ; Transfusions. Complications. Transfusion reactions. 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M.</creatorcontrib><title>Influence of cryopreservation on human periodontal ligament cells in vitro</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Banking</addtitle><addtitle>Cell Tissue Bank</addtitle><description>Cryopreservation of teeth before autotransplantation may create new possibilities in dentistry. The purpose of this study was to examine the effect of a standardised cryopreservation procedure on human periodontal ligament (PDL) cell cultures. Human PDL fibroblasts obtained from immature third molars of 11 patients were cultured and divided into two groups. The experimental group was cryopreserved and cultured after thawing. The control group was cultured without cryopreservation. A comparison was made between cryopreserved and control cells. To evaluate possible differences in the characteristics of the fibroblasts, the cells in both groups were tested for viability (membrane integrity), growth capacity and alkaline phosphatase (ALP) expression. The Wilcoxon test for paired comparison between cryopreserved and non-cryopreserved cells was performed for each characteristic. The results showed that membrane integrity of cells was not influenced by cryopreservation. There was no statistically significant difference in growth capacity between cryopreserved and control cells. Non-cryopreserved cells were slightly stronger positive for ALP, but the difference was not statistically significant. From these experiments it can be concluded that the observed parameters are not influenced by cryopreservation.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell Membrane - pathology</subject><subject>Cell Membrane - physiology</subject><subject>Cell Proliferation</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Comparative studies</subject><subject>Cryopreservation - methods</subject><subject>Cryotherapy</subject><subject>Fibroblasts - enzymology</subject><subject>Fibroblasts - pathology</subject><subject>Fibroblasts - physiology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Ligaments</subject><subject>Medical sciences</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - enzymology</subject><subject>Periodontal Ligament - physiology</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Teeth</subject><subject>Temperature effects</subject><subject>Transfusions. Complications. Transfusion reactions. 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M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of cryopreservation on human periodontal ligament cells in vitro</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Banking</stitle><addtitle>Cell Tissue Bank</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>9</volume><issue>1</issue><spage>11</spage><epage>18</epage><pages>11-18</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>Cryopreservation of teeth before autotransplantation may create new possibilities in dentistry. The purpose of this study was to examine the effect of a standardised cryopreservation procedure on human periodontal ligament (PDL) cell cultures. Human PDL fibroblasts obtained from immature third molars of 11 patients were cultured and divided into two groups. The experimental group was cryopreserved and cultured after thawing. The control group was cultured without cryopreservation. A comparison was made between cryopreserved and control cells. To evaluate possible differences in the characteristics of the fibroblasts, the cells in both groups were tested for viability (membrane integrity), growth capacity and alkaline phosphatase (ALP) expression. The Wilcoxon test for paired comparison between cryopreserved and non-cryopreserved cells was performed for each characteristic. The results showed that membrane integrity of cells was not influenced by cryopreservation. There was no statistically significant difference in growth capacity between cryopreserved and control cells. Non-cryopreserved cells were slightly stronger positive for ALP, but the difference was not statistically significant. From these experiments it can be concluded that the observed parameters are not influenced by cryopreservation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>17541731</pmid><doi>10.1007/s10561-007-9047-y</doi><tpages>8</tpages></addata></record> |
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| subjects | Adolescent Adult Alkaline Phosphatase - metabolism Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Biomedical and Life Sciences Biomedicine Bone marrow, stem cells transplantation. Graft versus host reaction Cell Biology Cell culture Cell Membrane - pathology Cell Membrane - physiology Cell Proliferation Cell Survival - physiology Cells, Cultured Comparative studies Cryopreservation - methods Cryotherapy Fibroblasts - enzymology Fibroblasts - pathology Fibroblasts - physiology Humans Life Sciences Ligaments Medical sciences Periodontal Ligament - cytology Periodontal Ligament - enzymology Periodontal Ligament - physiology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Teeth Temperature effects Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplant Surgery |
| title | Influence of cryopreservation on human periodontal ligament cells in vitro |
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