Derivation of a New Human Embryonic Stem Cell Line, Endeavour-1, and Its Clonal Propagation

Here we describe the derivation of a novel human embryonic stem (hES) cell line, Endeavour-1 (E1), its four new clonal lines (E1C1, E1C2, E1C3, E1C4), and their characterization. E1 and its clonal lines are propagated on human fetal fibroblasts (HFFs) derived and grown in a largely serum-free medium...

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Veröffentlicht in:	Stem cells and development 2008-02, Vol.17 (1), p.41-52
Hauptverfasser:	Sidhu, Kuldip S., Ryan, John P., Tuch, Bernie E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomarkers - analysis Cell culture Cell Line Cell Proliferation Clone Cells Embryonic stem cells Embryonic Stem Cells - cytology Humans Methods Original Research Reports Physiological aspects Polymerase chain reaction Tissue Culture Techniques
Online-Zugang:	Volltext
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