Cell culture and animal infection with distinct Trypanosoma cruzi strains expressing red and green fluorescent proteins

Different strains of Trypanosoma cruzi were transfected with an expression vector that allows the integration of green fluorescent protein (GFP) and red fluorescent protein (RFP) genes into the β-tubulin locus by homologous recombination. The sites of integration of the GFP and RFP markers were dete...

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Veröffentlicht in:	International journal for parasitology 2008-03, Vol.38 (3-4), p.289-297
Hauptverfasser:	Pires, S.F., DaRocha, W.D., Freitas, J.M., Oliveira, L.A., Kitten, G.T., Machado, C.R., Pena, S.D.J., Chiari, E., Macedo, A.M., Teixeira, S.M.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Chagas disease Chagas Disease - parasitology Chlorocebus aethiops Electrophoresis, Polyacrylamide Gel Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Expression Green fluorescent protein Green Fluorescent Proteins - genetics Humans Immunoblotting - methods Interferon-gamma - genetics Life cycle. Host-agent relationship. Pathogenesis Luminescent Proteins - genetics Mice Mice, Knockout Microscopy, Confocal Models, Animal Parasitology - methods Protozoa Red Fluorescent Protein Transfection - methods Trypanosoma cruzi Trypanosoma cruzi - genetics Vero Cells
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