Quantitative estimation of parthenolide in Tanacetum parthenium (L.) Schultz-Bip. cultivated in Egypt

Parthenolide, a germacranolide-type sesquiterpene lactone, was estimated in Tanacetum parthenium (L.) cultivated in Egypt by using colorimetric, planar chromatographic, and high-performance liquid chromatographic (HPLC) methods. Parthenolide levels in the open-field herb and aseptically germinated s...

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Veröffentlicht in:Journal of AOAC International 2007, Vol.90 (1), p.21-27
Hauptverfasser: El-Shamy, A.M, El-Hawary, S.S, Rateb, M.E.M
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creator El-Shamy, A.M
El-Hawary, S.S
Rateb, M.E.M
description Parthenolide, a germacranolide-type sesquiterpene lactone, was estimated in Tanacetum parthenium (L.) cultivated in Egypt by using colorimetric, planar chromatographic, and high-performance liquid chromatographic (HPLC) methods. Parthenolide levels in the open-field herb and aseptically germinated shoots were also compared by using the HPLC method. Parthenolide was produced and estimated for the first time in the callus culture of the plant. In addition, 2 Egyptian market preparations were analyzed for their parthenolide content by using the HPLC method. The relative standard deviations were 0.093, 0.095, and 0.098% (n = 5, 5, and 7, respectively), and the corresponding recoveries were 98.2, 98.9, and 99.4% for the colorimetric, planar chromatographic, and HPLC determinations, respectively.
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source Oxford University Press Journals All Titles (1996-Current); MEDLINE
subjects Biological and medical sciences
callus
callus culture
Chemical properties
Chromatography, High Pressure Liquid
Colorimetry
cultivars
Egypt
Feverfew
Food industries
Fundamental and applied biological sciences. Psychology
herbs
High performance liquid chromatography
Lactones
medicinal plants
Methods
NF-kappa B - antagonists & inhibitors
plant extracts
Properties
reversed-phase high performance liquid chromatography
Sesquiterpenes - analysis
Sesquiterpenes - isolation & purification
sesquiterpenoids
shoots
Spectrophotometry
Tanacetum parthenium
Tanacetum parthenium - chemistry
title Quantitative estimation of parthenolide in Tanacetum parthenium (L.) Schultz-Bip. cultivated in Egypt
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