Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A)
Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil Correspondence Maria Elisabete da Silva Barros betebarros{at}ef.ufop.br Received 25 January 2006 Accepted 12 December 2006 Onychomycosis is a common adult human myco...
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| Veröffentlicht in: | Journal of medical microbiology 2007-04, Vol.56 (4), p.514-518 |
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| creator | Barros, Maria Elisabete da Silva Santos, Daniel de Assis Hamdan, Junia Soares |
| description | Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Correspondence Maria Elisabete da Silva Barros betebarros{at}ef.ufop.br
Received 25 January 2006
Accepted 12 December 2006
Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most common causative agent. Many antimycotic agents are safe and highly effective for the treatment of dermatophytosis, and are available for clinical practice. Successful treatment depends on the ability of antifungal drugs to eradicate the fungal isolates. The aim of this work was to determine the MICs of four antifungal drugs (fluconazole, itraconazole, terbinafine and griseofulvin) recognized for ungual dermatophytosis treatment caused by Trichophyton species, especially Trichophyton mentagrophytes and Trichophyton rubrum . MICs were determined using a broth microdilution method in accordance with Clinical and Laboratory Standards Institute approved standard M38-A with some modifications, such as an incubation temperature of 28 °C, an incubation time of 7 days and inocula constituted of only microconidia. The results showed that the activities of terbinafine and itraconazole were significantly higher (MICs of |
| doi_str_mv | 10.1099/jmm.0.46542-0 |
| format | Article |
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Correspondence Maria Elisabete da Silva Barros betebarros{at}ef.ufop.br
Received 25 January 2006
Accepted 12 December 2006
Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most common causative agent. Many antimycotic agents are safe and highly effective for the treatment of dermatophytosis, and are available for clinical practice. Successful treatment depends on the ability of antifungal drugs to eradicate the fungal isolates. The aim of this work was to determine the MICs of four antifungal drugs (fluconazole, itraconazole, terbinafine and griseofulvin) recognized for ungual dermatophytosis treatment caused by Trichophyton species, especially Trichophyton mentagrophytes and Trichophyton rubrum . MICs were determined using a broth microdilution method in accordance with Clinical and Laboratory Standards Institute approved standard M38-A with some modifications, such as an incubation temperature of 28 °C, an incubation time of 7 days and inocula constituted of only microconidia. The results showed that the activities of terbinafine and itraconazole were significantly higher (MICs of <0.0070.031 and 0.0150.25 µg ml 1 , respectively) than other tested agents. All isolates had reduced susceptibility to fluconazole (164 µg ml 1 ). The MIC of griseofulvin varied among strains (MICs of 0.0621 µg ml 1 ). The parameters adopted to perform susceptibility testing of T. rubrum and T. mentagrophytes to antifungal agents appeared to be suitable and reliable, and could contribute to the possible development of a standard protocol.
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Correspondence Maria Elisabete da Silva Barros betebarros{at}ef.ufop.br
Received 25 January 2006
Accepted 12 December 2006
Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most common causative agent. Many antimycotic agents are safe and highly effective for the treatment of dermatophytosis, and are available for clinical practice. Successful treatment depends on the ability of antifungal drugs to eradicate the fungal isolates. The aim of this work was to determine the MICs of four antifungal drugs (fluconazole, itraconazole, terbinafine and griseofulvin) recognized for ungual dermatophytosis treatment caused by Trichophyton species, especially Trichophyton mentagrophytes and Trichophyton rubrum . MICs were determined using a broth microdilution method in accordance with Clinical and Laboratory Standards Institute approved standard M38-A with some modifications, such as an incubation temperature of 28 °C, an incubation time of 7 days and inocula constituted of only microconidia. The results showed that the activities of terbinafine and itraconazole were significantly higher (MICs of <0.0070.031 and 0.0150.25 µg ml 1 , respectively) than other tested agents. All isolates had reduced susceptibility to fluconazole (164 µg ml 1 ). The MIC of griseofulvin varied among strains (MICs of 0.0621 µg ml 1 ). The parameters adopted to perform susceptibility testing of T. rubrum and T. mentagrophytes to antifungal agents appeared to be suitable and reliable, and could contribute to the possible development of a standard protocol.
Abbreviations: CLSI, Clinical and Laboratory Standards Institute.</description><subject>Antifungal Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Resistance, Fungal</subject><subject>Fluconazole - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Itraconazole - pharmacology</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Naphthalenes - pharmacology</subject><subject>Onychomycosis - microbiology</subject><subject>Terbinafine</subject><subject>Trichophyton</subject><subject>Trichophyton - drug effects</subject><subject>Trichophyton mentagrophytes</subject><subject>Trichophyton rubrum</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EotPCki3yhtcigx-xEy-rUQuVBrGgrC3HdhJXSTzYcdH8J34kTmakihWrq3v86d7rcwB4g9EWIyE-P4zjFm1LzkpSoGdgg8uKFoyX5XOwQYiQgnDMLsBljA8I4YpS8RJc5FqVtaAb8OfmUQ1Jzc5P0LcwpqjtYXaNG9x8XJT74HTvD_1xzsRop1l1YW1thGoy_76H1IQ0Qj24yWk1QBf9oBZy9hmeXZumLssmpC7CFN3UQQVHb1zrrIG7_Y87ODodsjCk9aTRzr038OM3WhfXn16BF60aon19rlfg5-3N_e5rsf_-5W53vS90SehcaCNEWWtmaVvzpm2ptQY32FBqrCCtZiQrmhtaG96I2hqNsFWcZaMIZZzRK_D-NPcQ_K9k4yxHl30ZBjVZn6KsEBGEE_pfEIuaUVqhDBYnMH8uxmBbeQhuVOEoMZJLjjLnKJFcc5QL__Y8ODWjNU_0ObgMvDsDKman26Am7eITV_OK4pX7cOJ61_W_XbCys9PqceP8spRxWUqGS_oXo_S4kQ</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Barros, Maria Elisabete da Silva</creator><creator>Santos, Daniel de Assis</creator><creator>Hamdan, Junia Soares</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A)</title><author>Barros, Maria Elisabete da Silva ; Santos, Daniel de Assis ; Hamdan, Junia Soares</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-cd9948c5e3f86bff3eed1b1d33de92fc523eec6d38d6b98edc01ea65615235653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antifungal Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Resistance, Fungal</topic><topic>Fluconazole - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Itraconazole - pharmacology</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Naphthalenes - pharmacology</topic><topic>Onychomycosis - microbiology</topic><topic>Terbinafine</topic><topic>Trichophyton</topic><topic>Trichophyton - drug effects</topic><topic>Trichophyton mentagrophytes</topic><topic>Trichophyton rubrum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barros, Maria Elisabete da Silva</creatorcontrib><creatorcontrib>Santos, Daniel de Assis</creatorcontrib><creatorcontrib>Hamdan, Junia Soares</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barros, Maria Elisabete da Silva</au><au>Santos, Daniel de Assis</au><au>Hamdan, Junia Soares</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A)</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>56</volume><issue>4</issue><spage>514</spage><epage>518</epage><pages>514-518</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Correspondence Maria Elisabete da Silva Barros betebarros{at}ef.ufop.br
Received 25 January 2006
Accepted 12 December 2006
Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most common causative agent. Many antimycotic agents are safe and highly effective for the treatment of dermatophytosis, and are available for clinical practice. Successful treatment depends on the ability of antifungal drugs to eradicate the fungal isolates. The aim of this work was to determine the MICs of four antifungal drugs (fluconazole, itraconazole, terbinafine and griseofulvin) recognized for ungual dermatophytosis treatment caused by Trichophyton species, especially Trichophyton mentagrophytes and Trichophyton rubrum . MICs were determined using a broth microdilution method in accordance with Clinical and Laboratory Standards Institute approved standard M38-A with some modifications, such as an incubation temperature of 28 °C, an incubation time of 7 days and inocula constituted of only microconidia. The results showed that the activities of terbinafine and itraconazole were significantly higher (MICs of <0.0070.031 and 0.0150.25 µg ml 1 , respectively) than other tested agents. All isolates had reduced susceptibility to fluconazole (164 µg ml 1 ). The MIC of griseofulvin varied among strains (MICs of 0.0621 µg ml 1 ). The parameters adopted to perform susceptibility testing of T. rubrum and T. mentagrophytes to antifungal agents appeared to be suitable and reliable, and could contribute to the possible development of a standard protocol.
Abbreviations: CLSI, Clinical and Laboratory Standards Institute.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17374893</pmid><doi>10.1099/jmm.0.46542-0</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2615 |
| ispartof | Journal of medical microbiology, 2007-04, Vol.56 (4), p.514-518 |
| issn | 0022-2615 1473-5644 |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antifungal Agents - pharmacology Biological and medical sciences Dose-Response Relationship, Drug Drug Resistance, Fungal Fluconazole - pharmacology Fundamental and applied biological sciences. Psychology Humans Infectious diseases Itraconazole - pharmacology Medical sciences Microbial Sensitivity Tests Microbiology Miscellaneous Mycology Naphthalenes - pharmacology Onychomycosis - microbiology Terbinafine Trichophyton Trichophyton - drug effects Trichophyton mentagrophytes Trichophyton rubrum |
| title | Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A) |
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