Comparison of a novel semi-nested polymerase chain reaction (PCR) with a uniplex PCR for the detection of Acanthamoeba genome in corneal scrapings

A semi-nested polymerase chain reaction (snPCR) was developed to improve the sensitivity of detection of Acanthamoeba sp. genome from corneal scrapings of Acanthamoeba keratitis patients. The snPCR was developed using a laboratory designed inner forward primer targeting the 450-bp product of the 18s...

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Veröffentlicht in:	Parasitology research (1987) 2007-05, Vol.100 (6), p.1303-1309
Hauptverfasser:	Dhivya, Subramanian, Madhavan, Hajib Naraharirao, Rao, Ch. Mohan, Rao, K. Sridhar, Ramchander, P. V, Therese, Kulandai Lily, Malathi, Jambulingam
Format:	Artikel
Sprache:	eng
Schlagworte:	Acanthamoeba Acanthamoeba - genetics Acanthamoeba - isolation & purification Animals Base Sequence Biological and medical sciences Cornea - parasitology DNA, Protozoan - analysis Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Genome, Protozoan - genetics Invertebrates Molecular Sequence Data Polymerase Chain Reaction - methods Sensitivity and Specificity
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