Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis
Respiratory syncytial virus (RSV) glycoprotein G mimics fractalkine, a CX3C chemokine, which mediates chemotaxis of leukocytes expressing its receptor, CX3CR1. The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN‐γ in CX3CR1‐expressing periph...
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| Veröffentlicht in: | Pediatric allergy and immunology 2008-03, Vol.19 (2), p.148-156 |
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| creator | Cepika, Alma-Martina Gagro, Alenka Bace, Ana Tjesic-Drinkovic, Dorian Kelecic, Jadranka Baricic-Voskresensky, Tamara Matic, Mladen Drazenovic, Vladimir Marinic, Igor Mlinaric-Galinovic, Gordana Tjesic-Drinkovic, Duska Vrtar, Zvonimir Rabatic, Sabina |
| description | Respiratory syncytial virus (RSV) glycoprotein G mimics fractalkine, a CX3C chemokine, which mediates chemotaxis of leukocytes expressing its receptor, CX3CR1. The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN‐γ in CX3CR1‐expressing peripheral blood CD8+ T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age‐ and sex‐matched healthy controls (n = 15). Perforin expression and IFN‐γ secretion in CX3CR1+ CD8+ T cells were assessed by four‐color flow cytometry. The NF‐κB p50 and p65 subunit levels were also determined as markers of RSV‐induced inflammation. Study results showed perforin and CX3CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN‐γ secretion and NF‐κB binding activity between two time‐points in RSV‐infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute‐phase CX3CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX3CR1 expression. |
| doi_str_mv | 10.1111/j.1399-3038.2007.00611.x |
| format | Article |
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The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN‐γ in CX3CR1‐expressing peripheral blood CD8+ T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age‐ and sex‐matched healthy controls (n = 15). Perforin expression and IFN‐γ secretion in CX3CR1+ CD8+ T cells were assessed by four‐color flow cytometry. The NF‐κB p50 and p65 subunit levels were also determined as markers of RSV‐induced inflammation. Study results showed perforin and CX3CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN‐γ secretion and NF‐κB binding activity between two time‐points in RSV‐infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute‐phase CX3CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX3CR1 expression.</description><identifier>ISSN: 0905-6157</identifier><identifier>EISSN: 1399-3038</identifier><identifier>DOI: 10.1111/j.1399-3038.2007.00611.x</identifier><identifier>PMID: 18257903</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acute Disease ; Biological and medical sciences ; Biomarkers - metabolism ; Bronchiolitis, Viral - blood ; Bronchiolitis, Viral - immunology ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - secretion ; Cell Nucleus - metabolism ; Convalescence ; CX3C Chemokine Receptor 1 ; CX3CR1 ; Enzyme-Linked Immunosorbent Assay ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Infant ; Infant, Newborn ; infants ; Interferon-gamma - biosynthesis ; Interferon-gamma - secretion ; interferon-γ ; Male ; Medical sciences ; perforin ; Perforin - biosynthesis ; Receptors, Chemokine - biosynthesis ; Respiratory syncytial virus ; Respiratory Syncytial Virus Infections - blood ; Respiratory Syncytial Virus Infections - immunology ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. 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The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN‐γ in CX3CR1‐expressing peripheral blood CD8+ T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age‐ and sex‐matched healthy controls (n = 15). Perforin expression and IFN‐γ secretion in CX3CR1+ CD8+ T cells were assessed by four‐color flow cytometry. The NF‐κB p50 and p65 subunit levels were also determined as markers of RSV‐induced inflammation. Study results showed perforin and CX3CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN‐γ secretion and NF‐κB binding activity between two time‐points in RSV‐infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute‐phase CX3CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX3CR1 expression.</description><subject>Acute Disease</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Bronchiolitis, Viral - blood</subject><subject>Bronchiolitis, Viral - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - secretion</subject><subject>Cell Nucleus - metabolism</subject><subject>Convalescence</subject><subject>CX3C Chemokine Receptor 1</subject><subject>CX3CR1</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>infants</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - secretion</subject><subject>interferon-γ</subject><subject>Male</subject><subject>Medical sciences</subject><subject>perforin</subject><subject>Perforin - biosynthesis</subject><subject>Receptors, Chemokine - biosynthesis</subject><subject>Respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus Infections - blood</subject><subject>Respiratory Syncytial Virus Infections - immunology</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>infants</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - secretion</topic><topic>interferon-γ</topic><topic>Male</topic><topic>Medical sciences</topic><topic>perforin</topic><topic>Perforin - biosynthesis</topic><topic>Receptors, Chemokine - biosynthesis</topic><topic>Respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus Infections - blood</topic><topic>Respiratory Syncytial Virus Infections - immunology</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cepika, Alma-Martina</creatorcontrib><creatorcontrib>Gagro, Alenka</creatorcontrib><creatorcontrib>Bace, Ana</creatorcontrib><creatorcontrib>Tjesic-Drinkovic, Dorian</creatorcontrib><creatorcontrib>Kelecic, Jadranka</creatorcontrib><creatorcontrib>Baricic-Voskresensky, Tamara</creatorcontrib><creatorcontrib>Matic, Mladen</creatorcontrib><creatorcontrib>Drazenovic, Vladimir</creatorcontrib><creatorcontrib>Marinic, Igor</creatorcontrib><creatorcontrib>Mlinaric-Galinovic, Gordana</creatorcontrib><creatorcontrib>Tjesic-Drinkovic, Duska</creatorcontrib><creatorcontrib>Vrtar, Zvonimir</creatorcontrib><creatorcontrib>Rabatic, Sabina</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric allergy and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cepika, Alma-Martina</au><au>Gagro, Alenka</au><au>Bace, Ana</au><au>Tjesic-Drinkovic, Dorian</au><au>Kelecic, Jadranka</au><au>Baricic-Voskresensky, Tamara</au><au>Matic, Mladen</au><au>Drazenovic, Vladimir</au><au>Marinic, Igor</au><au>Mlinaric-Galinovic, Gordana</au><au>Tjesic-Drinkovic, Duska</au><au>Vrtar, Zvonimir</au><au>Rabatic, Sabina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis</atitle><jtitle>Pediatric allergy and immunology</jtitle><addtitle>Pediatr Allergy Immunol</addtitle><date>2008-03</date><risdate>2008</risdate><volume>19</volume><issue>2</issue><spage>148</spage><epage>156</epage><pages>148-156</pages><issn>0905-6157</issn><eissn>1399-3038</eissn><abstract>Respiratory syncytial virus (RSV) glycoprotein G mimics fractalkine, a CX3C chemokine, which mediates chemotaxis of leukocytes expressing its receptor, CX3CR1. The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN‐γ in CX3CR1‐expressing peripheral blood CD8+ T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age‐ and sex‐matched healthy controls (n = 15). Perforin expression and IFN‐γ secretion in CX3CR1+ CD8+ T cells were assessed by four‐color flow cytometry. The NF‐κB p50 and p65 subunit levels were also determined as markers of RSV‐induced inflammation. Study results showed perforin and CX3CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN‐γ secretion and NF‐κB binding activity between two time‐points in RSV‐infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute‐phase CX3CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX3CR1 expression.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18257903</pmid><doi>10.1111/j.1399-3038.2007.00611.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute Disease Biological and medical sciences Biomarkers - metabolism Bronchiolitis, Viral - blood Bronchiolitis, Viral - immunology CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - secretion Cell Nucleus - metabolism Convalescence CX3C Chemokine Receptor 1 CX3CR1 Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Infant Infant, Newborn infants Interferon-gamma - biosynthesis Interferon-gamma - secretion interferon-γ Male Medical sciences perforin Perforin - biosynthesis Receptors, Chemokine - biosynthesis Respiratory syncytial virus Respiratory Syncytial Virus Infections - blood Respiratory Syncytial Virus Infections - immunology Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis |
| title | Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis |
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