Real-time polymerase chain reaction, in situ hybridization and immunohistochemical localization of insulin-like growth factor-I and myostatin during development of Dicentrarchus labrax (Pisces: Osteichthyes)

The distribution of insulin-like growth factor-I (IGF-I) and myostatin (MSTN) was investigated in sea bass ( Dicentrarchus labrax ) by real-time polymerase chain reaction (PCR), in situ hybridization (ISH) and immunohistochemistry. Real-time PCR indicated that IGF-I mRNA increased from the second da...

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Veröffentlicht in:	Cell and tissue research 2008-03, Vol.331 (3), p.643-658
Hauptverfasser:	Patruno, Marco, Sivieri, Susanna, Poltronieri, Carlo, Sacchetto, Roberta, Maccatrozzo, Lisa, Martinello, Tiziana, Funkenstein, Bruria, Radaelli, Giuseppe
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bass - genetics Bass - growth & development Bass - metabolism Biomedical and Life Sciences Biomedicine Cartilage - growth & development Cartilage - metabolism Cellular biology Epithelium - growth & development Epithelium - metabolism Fish Fish Proteins - genetics Fish Proteins - metabolism Gene Expression Regulation, Developmental Human Genetics Immunohistochemistry Immunology In Situ Hybridization Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - metabolism Intestines - growth & development Intestines - metabolism Larva - genetics Larva - growth & development Larva - metabolism Liver - growth & development Liver - metabolism Marine biology Molecular Medicine Muscles - metabolism Myostatin Proteomics Regular Article Reverse Transcriptase Polymerase Chain Reaction RNA polymerase Transforming Growth Factor beta - genetics Transforming Growth Factor beta - metabolism Yolk Sac - growth & development Yolk Sac - metabolism
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