The clinical application of autologous bioengineered skin based on a hyaluronic acid scaffold
Abstract The aim of this work was to generate an in vitro skin substitute harbouring autologous fibroblasts, keratinocytes and melanocytes, to establish a new one-step clinical method in problems associated with skin disorders. Here we present a case of a nine-year-old girl with a congenital giant n...
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| Veröffentlicht in: | Biomaterials 2008-04, Vol.29 (11), p.1620-1629 |
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| creator | Scuderi, Nicolò Onesti, Maria G Bistoni, Giovanni Ceccarelli, Simona Rotolo, Sabrina Angeloni, Antonio Marchese, Cinzia |
| description | Abstract The aim of this work was to generate an in vitro skin substitute harbouring autologous fibroblasts, keratinocytes and melanocytes, to establish a new one-step clinical method in problems associated with skin disorders. Here we present a case of a nine-year-old girl with a congenital giant nevus treated by surgical approach, with primary co-cultures of keratinocytes, melanocytes and fibroblasts obtained from autologous skin biopsy. Generally these lesions need to be removed to avoid the risk of transformation into malignant melanoma. With this purpose we analyzed the melanocytes contained in the new skin substitute for the presence of genetic alterations correlated to increased risk for melanoma. The organotypical cultures were designed including an engineered scaffold of a non-woven mesh of hyaluronic acid (HYAFF® 11). This biomaterial has been previously demonstrated to be the most suitable to maintain polarity and to support the in vitro constructs. Six dermal–epidermal skin substitutes were transplanted and 14 days after surgery the re-epithelialized area was about 90%. Our results suggest that this new dermal–epidermal construct not only reduces hospitalization time and ameliorates scar retraction, but might also represent a solution for the high risk of developing a tumour derived from the original nevus. |
| doi_str_mv | 10.1016/j.biomaterials.2007.12.024 |
| format | Article |
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Here we present a case of a nine-year-old girl with a congenital giant nevus treated by surgical approach, with primary co-cultures of keratinocytes, melanocytes and fibroblasts obtained from autologous skin biopsy. Generally these lesions need to be removed to avoid the risk of transformation into malignant melanoma. With this purpose we analyzed the melanocytes contained in the new skin substitute for the presence of genetic alterations correlated to increased risk for melanoma. The organotypical cultures were designed including an engineered scaffold of a non-woven mesh of hyaluronic acid (HYAFF® 11). This biomaterial has been previously demonstrated to be the most suitable to maintain polarity and to support the in vitro constructs. Six dermal–epidermal skin substitutes were transplanted and 14 days after surgery the re-epithelialized area was about 90%. Our results suggest that this new dermal–epidermal construct not only reduces hospitalization time and ameliorates scar retraction, but might also represent a solution for the high risk of developing a tumour derived from the original nevus.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2007.12.024</identifier><identifier>PMID: 18201759</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Autologous cell ; Base Sequence ; Bioartificial Organs ; Biomedical Engineering ; Biopsy ; Cells, Cultured ; Child ; Co-culture ; Cyclin-Dependent Kinase Inhibitor Proteins - genetics ; Cyclin-Dependent Kinase Inhibitor Proteins - metabolism ; Dentistry ; Female ; Fibroblasts ; Gene Deletion ; Humans ; Hyaluronic Acid ; Hyaluronic acid scaffold ; Keratinocytes ; Melanocytes - metabolism ; Mutation - genetics ; Proto-Oncogene Proteins B-raf - genetics ; Proto-Oncogene Proteins B-raf - metabolism ; Skin Diseases - genetics ; Skin Diseases - metabolism ; Skin Diseases - pathology ; Skin Diseases - surgery ; Skin Transplantation - methods ; Tissue Culture Techniques ; Tissue Scaffolds ; Transplantation ; Transplantation, Autologous</subject><ispartof>Biomaterials, 2008-04, Vol.29 (11), p.1620-1629</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-fc8b9fdad1e5a224b29e720d1c10d8ab2e70c284ed868ad07507a168d7c826e03</citedby><cites>FETCH-LOGICAL-c495t-fc8b9fdad1e5a224b29e720d1c10d8ab2e70c284ed868ad07507a168d7c826e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961207009891$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18201759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scuderi, Nicolò</creatorcontrib><creatorcontrib>Onesti, Maria G</creatorcontrib><creatorcontrib>Bistoni, Giovanni</creatorcontrib><creatorcontrib>Ceccarelli, Simona</creatorcontrib><creatorcontrib>Rotolo, Sabrina</creatorcontrib><creatorcontrib>Angeloni, Antonio</creatorcontrib><creatorcontrib>Marchese, Cinzia</creatorcontrib><title>The clinical application of autologous bioengineered skin based on a hyaluronic acid scaffold</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract The aim of this work was to generate an in vitro skin substitute harbouring autologous fibroblasts, keratinocytes and melanocytes, to establish a new one-step clinical method in problems associated with skin disorders. Here we present a case of a nine-year-old girl with a congenital giant nevus treated by surgical approach, with primary co-cultures of keratinocytes, melanocytes and fibroblasts obtained from autologous skin biopsy. Generally these lesions need to be removed to avoid the risk of transformation into malignant melanoma. With this purpose we analyzed the melanocytes contained in the new skin substitute for the presence of genetic alterations correlated to increased risk for melanoma. The organotypical cultures were designed including an engineered scaffold of a non-woven mesh of hyaluronic acid (HYAFF® 11). This biomaterial has been previously demonstrated to be the most suitable to maintain polarity and to support the in vitro constructs. Six dermal–epidermal skin substitutes were transplanted and 14 days after surgery the re-epithelialized area was about 90%. Our results suggest that this new dermal–epidermal construct not only reduces hospitalization time and ameliorates scar retraction, but might also represent a solution for the high risk of developing a tumour derived from the original nevus.</description><subject>Advanced Basic Science</subject><subject>Autologous cell</subject><subject>Base Sequence</subject><subject>Bioartificial Organs</subject><subject>Biomedical Engineering</subject><subject>Biopsy</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Co-culture</subject><subject>Cyclin-Dependent Kinase Inhibitor Proteins - genetics</subject><subject>Cyclin-Dependent Kinase Inhibitor Proteins - metabolism</subject><subject>Dentistry</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Gene Deletion</subject><subject>Humans</subject><subject>Hyaluronic Acid</subject><subject>Hyaluronic acid scaffold</subject><subject>Keratinocytes</subject><subject>Melanocytes - metabolism</subject><subject>Mutation - genetics</subject><subject>Proto-Oncogene Proteins B-raf - genetics</subject><subject>Proto-Oncogene Proteins B-raf - metabolism</subject><subject>Skin Diseases - genetics</subject><subject>Skin Diseases - metabolism</subject><subject>Skin Diseases - pathology</subject><subject>Skin Diseases - surgery</subject><subject>Skin Transplantation - methods</subject><subject>Tissue Culture Techniques</subject><subject>Tissue Scaffolds</subject><subject>Transplantation</subject><subject>Transplantation, Autologous</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkFv1DAQhS0EotvCX0ARB25ZZmaT2OGAhAoUpEocKEdkOfak9TYbL3aCtP8eR7sIxKWcPJa_N2_kN0K8RFgjYPN6u-582JmJozdDWhOAXCOtgapHYoVKqrJuoX4sVoAVlW2DdCbOU9pCvkNFT8UZKgKUdbsS32_uuLCDH701Q2H2-yEXkw9jEfrCzFMYwm2YU5Edebz1I3NkV6R7PxadSbnMpCnuDmaYY8hNCmN9frem78PgnoknfR6Rn5_OC_Ht44eby0_l9Zerz5fvrktbtfVU9lZ1be-MQ64NUdVRy5LAoUVwynTEEiypip1qlHEga5AGG-WkVdQwbC7Eq2PffQw_Zk6T3vlkeRjMyHl6LYEUgmweBDeEGwUkHwQJ6ka1tFi_OYI2hpQi93of_c7Eg0bQS1x6q_-OSy9xaSSd48riFyeXudux-yM95ZOB90eA8-_99Bx1sp5Hy85HtpN2wf-fz9t_2vyO_J4PnLZhjuOiQZ2yQH9dFmfZG5AArWpx8wuHsMLr</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Scuderi, Nicolò</creator><creator>Onesti, Maria G</creator><creator>Bistoni, Giovanni</creator><creator>Ceccarelli, Simona</creator><creator>Rotolo, Sabrina</creator><creator>Angeloni, Antonio</creator><creator>Marchese, Cinzia</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20080401</creationdate><title>The clinical application of autologous bioengineered skin based on a hyaluronic acid scaffold</title><author>Scuderi, Nicolò ; Onesti, Maria G ; Bistoni, Giovanni ; Ceccarelli, Simona ; Rotolo, Sabrina ; Angeloni, Antonio ; Marchese, Cinzia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-fc8b9fdad1e5a224b29e720d1c10d8ab2e70c284ed868ad07507a168d7c826e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Advanced Basic Science</topic><topic>Autologous cell</topic><topic>Base Sequence</topic><topic>Bioartificial Organs</topic><topic>Biomedical Engineering</topic><topic>Biopsy</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Co-culture</topic><topic>Cyclin-Dependent Kinase Inhibitor Proteins - genetics</topic><topic>Cyclin-Dependent Kinase Inhibitor Proteins - metabolism</topic><topic>Dentistry</topic><topic>Female</topic><topic>Fibroblasts</topic><topic>Gene Deletion</topic><topic>Humans</topic><topic>Hyaluronic Acid</topic><topic>Hyaluronic acid scaffold</topic><topic>Keratinocytes</topic><topic>Melanocytes - metabolism</topic><topic>Mutation - genetics</topic><topic>Proto-Oncogene Proteins B-raf - genetics</topic><topic>Proto-Oncogene Proteins B-raf - metabolism</topic><topic>Skin Diseases - genetics</topic><topic>Skin Diseases - metabolism</topic><topic>Skin Diseases - pathology</topic><topic>Skin Diseases - surgery</topic><topic>Skin Transplantation - methods</topic><topic>Tissue Culture Techniques</topic><topic>Tissue Scaffolds</topic><topic>Transplantation</topic><topic>Transplantation, Autologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scuderi, Nicolò</creatorcontrib><creatorcontrib>Onesti, Maria G</creatorcontrib><creatorcontrib>Bistoni, Giovanni</creatorcontrib><creatorcontrib>Ceccarelli, Simona</creatorcontrib><creatorcontrib>Rotolo, Sabrina</creatorcontrib><creatorcontrib>Angeloni, Antonio</creatorcontrib><creatorcontrib>Marchese, Cinzia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scuderi, Nicolò</au><au>Onesti, Maria G</au><au>Bistoni, Giovanni</au><au>Ceccarelli, Simona</au><au>Rotolo, Sabrina</au><au>Angeloni, Antonio</au><au>Marchese, Cinzia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The clinical application of autologous bioengineered skin based on a hyaluronic acid scaffold</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>29</volume><issue>11</issue><spage>1620</spage><epage>1629</epage><pages>1620-1629</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract The aim of this work was to generate an in vitro skin substitute harbouring autologous fibroblasts, keratinocytes and melanocytes, to establish a new one-step clinical method in problems associated with skin disorders. Here we present a case of a nine-year-old girl with a congenital giant nevus treated by surgical approach, with primary co-cultures of keratinocytes, melanocytes and fibroblasts obtained from autologous skin biopsy. Generally these lesions need to be removed to avoid the risk of transformation into malignant melanoma. With this purpose we analyzed the melanocytes contained in the new skin substitute for the presence of genetic alterations correlated to increased risk for melanoma. The organotypical cultures were designed including an engineered scaffold of a non-woven mesh of hyaluronic acid (HYAFF® 11). This biomaterial has been previously demonstrated to be the most suitable to maintain polarity and to support the in vitro constructs. Six dermal–epidermal skin substitutes were transplanted and 14 days after surgery the re-epithelialized area was about 90%. 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| ispartof | Biomaterials, 2008-04, Vol.29 (11), p.1620-1629 |
| issn | 0142-9612 1878-5905 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Advanced Basic Science Autologous cell Base Sequence Bioartificial Organs Biomedical Engineering Biopsy Cells, Cultured Child Co-culture Cyclin-Dependent Kinase Inhibitor Proteins - genetics Cyclin-Dependent Kinase Inhibitor Proteins - metabolism Dentistry Female Fibroblasts Gene Deletion Humans Hyaluronic Acid Hyaluronic acid scaffold Keratinocytes Melanocytes - metabolism Mutation - genetics Proto-Oncogene Proteins B-raf - genetics Proto-Oncogene Proteins B-raf - metabolism Skin Diseases - genetics Skin Diseases - metabolism Skin Diseases - pathology Skin Diseases - surgery Skin Transplantation - methods Tissue Culture Techniques Tissue Scaffolds Transplantation Transplantation, Autologous |
| title | The clinical application of autologous bioengineered skin based on a hyaluronic acid scaffold |
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