Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes
A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of Trypanosoma cruzi onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a m...
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| Veröffentlicht in: | Journal of materials science. Materials in medicine 2008-02, Vol.19 (2), p.789-795 |
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| creator | Gonzalez, Verónica D. G. Gugliotta, Luis M. Giacomelli, Carla E. Meira, Gregorio R. |
| description | A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of
Trypanosoma cruzi
onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m
2
. In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m
2
, around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point). |
| doi_str_mv | 10.1007/s10856-006-0041-x |
| format | Article |
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Trypanosoma cruzi
onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m
2
. In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m
2
, around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point).</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-006-0041-x</identifier><identifier>PMID: 17665133</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Antibodies, Protozoan - chemistry ; Antibodies, Protozoan - immunology ; Antigens ; Antigens, Protozoan - chemistry ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Carboxylated ; Ceramics ; Chagas Disease - diagnosis ; Chemistry and Materials Science ; Composites ; Covalence ; Density ; Disease ; Glass ; Humans ; Immunoassay - instrumentation ; Immunologic Tests ; Joining ; Latex ; Latex - chemical synthesis ; Materials Science ; Materials Testing ; Medical diagnosis ; Natural Materials ; Polymer Sciences ; Proteins ; Regenerative Medicine/Tissue Engineering ; Serum Albumin, Bovine - chemistry ; Studies ; Surfaces and Interfaces ; Thin Films ; Trypanosoma cruzi ; Trypanosoma cruzi - immunology</subject><ispartof>Journal of materials science. Materials in medicine, 2008-02, Vol.19 (2), p.789-795</ispartof><rights>Springer Science+Business Media, LLC 2007</rights><rights>Springer Science+Business Media, LLC 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-2f7cafc14d7aa62ffd5dc64edab1dfa797dafb3ef4ae6bb4ee1810bc4796d3423</citedby><cites>FETCH-LOGICAL-c464t-2f7cafc14d7aa62ffd5dc64edab1dfa797dafb3ef4ae6bb4ee1810bc4796d3423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10856-006-0041-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10856-006-0041-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17665133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonzalez, Verónica D. G.</creatorcontrib><creatorcontrib>Gugliotta, Luis M.</creatorcontrib><creatorcontrib>Giacomelli, Carla E.</creatorcontrib><creatorcontrib>Meira, Gregorio R.</creatorcontrib><title>Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of
Trypanosoma cruzi
onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m
2
. In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m
2
, around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point).</description><subject>Animals</subject><subject>Antibodies, Protozoan - chemistry</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Antigens</subject><subject>Antigens, Protozoan - chemistry</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Carboxylated</subject><subject>Ceramics</subject><subject>Chagas Disease - diagnosis</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Covalence</subject><subject>Density</subject><subject>Disease</subject><subject>Glass</subject><subject>Humans</subject><subject>Immunoassay - instrumentation</subject><subject>Immunologic Tests</subject><subject>Joining</subject><subject>Latex</subject><subject>Latex - chemical synthesis</subject><subject>Materials Science</subject><subject>Materials Testing</subject><subject>Medical diagnosis</subject><subject>Natural Materials</subject><subject>Polymer Sciences</subject><subject>Proteins</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Studies</subject><subject>Surfaces and Interfaces</subject><subject>Thin Films</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - immunology</subject><issn>0957-4530</issn><issn>1573-4838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk1r3DAQhkVpSLZpfkAvRfRQcnGqb9m9haVpFxZyac9ClkaOg21tLRuc_vrI7EKg0OagEWieeQbEi9AHSm4oIfpLoqSUqiBkPYIWyxu0oVLzQpS8fIs2pJK6EJKTC_QupUeSoUrKc3RBtVKScr5Bf_Z2ggXHgNu-n4foW9sMMbUJhzhiDxO4qR0aPD0A3j7Yxibs2wQ2wVe8293kN-hbZzvs4nzoVjKr7DC1DQz4tuEKx2GK2NmxjstTl5d5vNYF0nt0FmyX4Op0X6Jfd99-bn8U-_vvu-3tvnBCialgQTsbHBVeW6tYCF56pwR4W1MfrK60t6HmEIQFVdcCgJaU1E7oSnkuGL9En4_ewxh_z5Am07fJQdfZAeKcjCZMSymrV0HOiBa0Uq-CjJRCaFZm8Pq_IFWCsUpwtTo__YU-xnkc8scYxihTjNLVR4-QG2NKIwRzGNvejk-GErNGwhwjYXIkzBoJs-SZjyfxXPfgXyZOGcgAOwIpt4YGxpfN_7Y-A8EBwsQ</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Gonzalez, Verónica D. 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Chemical coupling of antigen Ag36 onto carboxylated latexes</title><author>Gonzalez, Verónica D. G. ; Gugliotta, Luis M. ; Giacomelli, Carla E. ; Meira, Gregorio R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-2f7cafc14d7aa62ffd5dc64edab1dfa797dafb3ef4ae6bb4ee1810bc4796d3423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antibodies, Protozoan - chemistry</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Antigens</topic><topic>Antigens, Protozoan - chemistry</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical materials</topic><topic>Carboxylated</topic><topic>Ceramics</topic><topic>Chagas Disease - diagnosis</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Covalence</topic><topic>Density</topic><topic>Disease</topic><topic>Glass</topic><topic>Humans</topic><topic>Immunoassay - instrumentation</topic><topic>Immunologic Tests</topic><topic>Joining</topic><topic>Latex</topic><topic>Latex - chemical synthesis</topic><topic>Materials Science</topic><topic>Materials Testing</topic><topic>Medical diagnosis</topic><topic>Natural Materials</topic><topic>Polymer Sciences</topic><topic>Proteins</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Studies</topic><topic>Surfaces and Interfaces</topic><topic>Thin Films</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonzalez, Verónica D. G.</creatorcontrib><creatorcontrib>Gugliotta, Luis M.</creatorcontrib><creatorcontrib>Giacomelli, Carla E.</creatorcontrib><creatorcontrib>Meira, Gregorio R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>DELNET Engineering & Technology Collection</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of materials science. Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonzalez, Verónica D. G.</au><au>Gugliotta, Luis M.</au><au>Giacomelli, Carla E.</au><au>Meira, Gregorio R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>19</volume><issue>2</issue><spage>789</spage><epage>795</epage><pages>789-795</pages><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of
Trypanosoma cruzi
onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m
2
. In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m
2
, around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point).</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>17665133</pmid><doi>10.1007/s10856-006-0041-x</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Antibodies, Protozoan - chemistry Antibodies, Protozoan - immunology Antigens Antigens, Protozoan - chemistry Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Carboxylated Ceramics Chagas Disease - diagnosis Chemistry and Materials Science Composites Covalence Density Disease Glass Humans Immunoassay - instrumentation Immunologic Tests Joining Latex Latex - chemical synthesis Materials Science Materials Testing Medical diagnosis Natural Materials Polymer Sciences Proteins Regenerative Medicine/Tissue Engineering Serum Albumin, Bovine - chemistry Studies Surfaces and Interfaces Thin Films Trypanosoma cruzi Trypanosoma cruzi - immunology |
| title | Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes |
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