Anti-angiogenic effect of tetraacetyl-phytosphingosine
: In a search for the wound healing accelerators, we found that tetraacetyl‐phytosphingosine (TAPS), a sphingolipid metabolite produced by phytosphingosine acetylation, has significant inhibitory potential on healing of rabbit ear wound. As angiogenesis is fundamental to proper wound healing, we ex...
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| Veröffentlicht in: | Experimental dermatology 2007-04, Vol.16 (4), p.311-317 |
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| creator | Kwon, Yoo Bin Kim, Chang Deok Kim, Bo Joong Kim, Min-Young Park, Chang Seo Yoon, Tae-Jin Seo, Young-Joon Suhr, Ki-Beom Park, Jang-Kyu Lee, Jeung-Hoon |
| description | : In a search for the wound healing accelerators, we found that tetraacetyl‐phytosphingosine (TAPS), a sphingolipid metabolite produced by phytosphingosine acetylation, has significant inhibitory potential on healing of rabbit ear wound. As angiogenesis is fundamental to proper wound healing, we examined the effect of TAPS on angiogenesis using human umbilical vein endothelial cells cultured in vitro. TAPS markedly decreased vascular endothelial growth factor (VEGF)‐induced chemotactic migration and capillary‐like tube formation. Recognizing its inhibitory potential on angiogenesis, we further investigated the action mechanism of TAPS. TAPS significantly inhibited VEGF‐induced proteolytic enzyme production, including matrix metalloproteinase‐2, urokinase‐type plasminogen activator and plasminogen activator inhibitor‐1. TAPS also suppressed VEGF‐induced phosphorylation of p42/44 extracellular signal‐regulated kinase and c‐Jun N‐terminal kinase. In addition, TAPS abolished VEGF‐induced intracellular calcium increase, measured using laser scanning confocal microscopy. Together, these results suggest that TAPS exerts its inhibitory action on angiogenesis through the inhibition of mitogen‐activated protein kinase activation and intracellular calcium increase, thereby affecting the process of wound healing negatively. |
| doi_str_mv | 10.1111/j.1600-0625.2006.00530.x |
| format | Article |
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As angiogenesis is fundamental to proper wound healing, we examined the effect of TAPS on angiogenesis using human umbilical vein endothelial cells cultured in vitro. TAPS markedly decreased vascular endothelial growth factor (VEGF)‐induced chemotactic migration and capillary‐like tube formation. Recognizing its inhibitory potential on angiogenesis, we further investigated the action mechanism of TAPS. TAPS significantly inhibited VEGF‐induced proteolytic enzyme production, including matrix metalloproteinase‐2, urokinase‐type plasminogen activator and plasminogen activator inhibitor‐1. TAPS also suppressed VEGF‐induced phosphorylation of p42/44 extracellular signal‐regulated kinase and c‐Jun N‐terminal kinase. In addition, TAPS abolished VEGF‐induced intracellular calcium increase, measured using laser scanning confocal microscopy. Together, these results suggest that TAPS exerts its inhibitory action on angiogenesis through the inhibition of mitogen‐activated protein kinase activation and intracellular calcium increase, thereby affecting the process of wound healing negatively.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/j.1600-0625.2006.00530.x</identifier><identifier>PMID: 17359337</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetylation ; angiogenesis ; Animals ; Biological and medical sciences ; calcium ; Calcium - metabolism ; Cell Movement ; Cells, Cultured ; Dermatology ; Endothelial Cells - cytology ; Endothelial Cells - drug effects ; Endothelial Cells - enzymology ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Humans ; JNK Mitogen-Activated Protein Kinases - metabolism ; Matrix Metalloproteinase 2 - metabolism ; Medical sciences ; mitogen-activated protein kinase ; Neovascularization, Physiologic - drug effects ; Phosphorylation - drug effects ; Plasminogen Activator Inhibitor 1 - metabolism ; proteolytic enzyme ; Rabbits ; Signal Transduction - drug effects ; Sphingosine - analogs & derivatives ; Sphingosine - chemistry ; Sphingosine - pharmacology ; tetraacetyl-phytosphingosine ; Umbilical Veins - cytology ; Urokinase-Type Plasminogen Activator - metabolism ; Vascular Endothelial Growth Factor A - pharmacology ; Wound Healing - drug effects</subject><ispartof>Experimental dermatology, 2007-04, Vol.16 (4), p.311-317</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4340-e287b9e55663c65b7ded65b5be8cc8af677ec82f9a8e4728db4ac866416d5a943</citedby><cites>FETCH-LOGICAL-c4340-e287b9e55663c65b7ded65b5be8cc8af677ec82f9a8e4728db4ac866416d5a943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0625.2006.00530.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0625.2006.00530.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18589627$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17359337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kwon, Yoo Bin</creatorcontrib><creatorcontrib>Kim, Chang Deok</creatorcontrib><creatorcontrib>Kim, Bo Joong</creatorcontrib><creatorcontrib>Kim, Min-Young</creatorcontrib><creatorcontrib>Park, Chang Seo</creatorcontrib><creatorcontrib>Yoon, Tae-Jin</creatorcontrib><creatorcontrib>Seo, Young-Joon</creatorcontrib><creatorcontrib>Suhr, Ki-Beom</creatorcontrib><creatorcontrib>Park, Jang-Kyu</creatorcontrib><creatorcontrib>Lee, Jeung-Hoon</creatorcontrib><title>Anti-angiogenic effect of tetraacetyl-phytosphingosine</title><title>Experimental dermatology</title><addtitle>Exp Dermatol</addtitle><description>: In a search for the wound healing accelerators, we found that tetraacetyl‐phytosphingosine (TAPS), a sphingolipid metabolite produced by phytosphingosine acetylation, has significant inhibitory potential on healing of rabbit ear wound. As angiogenesis is fundamental to proper wound healing, we examined the effect of TAPS on angiogenesis using human umbilical vein endothelial cells cultured in vitro. TAPS markedly decreased vascular endothelial growth factor (VEGF)‐induced chemotactic migration and capillary‐like tube formation. Recognizing its inhibitory potential on angiogenesis, we further investigated the action mechanism of TAPS. TAPS significantly inhibited VEGF‐induced proteolytic enzyme production, including matrix metalloproteinase‐2, urokinase‐type plasminogen activator and plasminogen activator inhibitor‐1. TAPS also suppressed VEGF‐induced phosphorylation of p42/44 extracellular signal‐regulated kinase and c‐Jun N‐terminal kinase. In addition, TAPS abolished VEGF‐induced intracellular calcium increase, measured using laser scanning confocal microscopy. Together, these results suggest that TAPS exerts its inhibitory action on angiogenesis through the inhibition of mitogen‐activated protein kinase activation and intracellular calcium increase, thereby affecting the process of wound healing negatively.</description><subject>Acetylation</subject><subject>angiogenesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Dermatology</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - enzymology</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Humans</subject><subject>JNK Mitogen-Activated Protein Kinases - metabolism</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Medical sciences</subject><subject>mitogen-activated protein kinase</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Phosphorylation - drug effects</subject><subject>Plasminogen Activator Inhibitor 1 - metabolism</subject><subject>proteolytic enzyme</subject><subject>Rabbits</subject><subject>Signal Transduction - drug effects</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - chemistry</subject><subject>Sphingosine - pharmacology</subject><subject>tetraacetyl-phytosphingosine</subject><subject>Umbilical Veins - cytology</subject><subject>Urokinase-Type Plasminogen Activator - metabolism</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><subject>Wound Healing - drug effects</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkclOwzAURS0EgjL8AuoGdgkvcTxEYsPUMlSwARWxsRznpbikSYhT0f49CalgizfXks_1s44JGQbgB-06m_sBB_CAh8wPAbgPwCj4qy0y-D3YJgOIgXtcANsj-87NAQJBBdsle22ymFIxIPyiaKyni5ktZ1hYM8QsQ9MMy2zYYFNrbbBZ5171vm5KV73bYlY6W-Ah2cl07vBokwfkZXTzfHXrTZ7Gd1cXE89ENAIPQymSGBnjnBrOEpFi2gZLUBojdcaFQCPDLNYSIxHKNIm0kZxHAU-ZjiN6QE77e6u6_Fyia9TCOoN5rgssl04JCHnMgLag7EFTl87VmKmqtgtdr1UAqnOm5qpTozo1qnOmfpypVVs93sxYJgtM_4obSS1wsgG0MzrPal0Y6_44yWTMw44777kvm-P63w9QN6_X7aate33dugZXv3Vdfyje_ZuaPo7V6IFOg7f7SxXTb9OEliM</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Kwon, Yoo Bin</creator><creator>Kim, Chang Deok</creator><creator>Kim, Bo Joong</creator><creator>Kim, Min-Young</creator><creator>Park, Chang Seo</creator><creator>Yoon, Tae-Jin</creator><creator>Seo, Young-Joon</creator><creator>Suhr, Ki-Beom</creator><creator>Park, Jang-Kyu</creator><creator>Lee, Jeung-Hoon</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>Anti-angiogenic effect of tetraacetyl-phytosphingosine</title><author>Kwon, Yoo Bin ; Kim, Chang Deok ; Kim, Bo Joong ; Kim, Min-Young ; Park, Chang Seo ; Yoon, Tae-Jin ; Seo, Young-Joon ; Suhr, Ki-Beom ; Park, Jang-Kyu ; Lee, Jeung-Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4340-e287b9e55663c65b7ded65b5be8cc8af677ec82f9a8e4728db4ac866416d5a943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acetylation</topic><topic>angiogenesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Dermatology</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - enzymology</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Humans</topic><topic>JNK Mitogen-Activated Protein Kinases - metabolism</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Medical sciences</topic><topic>mitogen-activated protein kinase</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Phosphorylation - drug effects</topic><topic>Plasminogen Activator Inhibitor 1 - metabolism</topic><topic>proteolytic enzyme</topic><topic>Rabbits</topic><topic>Signal Transduction - drug effects</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - chemistry</topic><topic>Sphingosine - pharmacology</topic><topic>tetraacetyl-phytosphingosine</topic><topic>Umbilical Veins - cytology</topic><topic>Urokinase-Type Plasminogen Activator - metabolism</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwon, Yoo Bin</creatorcontrib><creatorcontrib>Kim, Chang Deok</creatorcontrib><creatorcontrib>Kim, Bo Joong</creatorcontrib><creatorcontrib>Kim, Min-Young</creatorcontrib><creatorcontrib>Park, Chang Seo</creatorcontrib><creatorcontrib>Yoon, Tae-Jin</creatorcontrib><creatorcontrib>Seo, Young-Joon</creatorcontrib><creatorcontrib>Suhr, Ki-Beom</creatorcontrib><creatorcontrib>Park, Jang-Kyu</creatorcontrib><creatorcontrib>Lee, Jeung-Hoon</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwon, Yoo Bin</au><au>Kim, Chang Deok</au><au>Kim, Bo Joong</au><au>Kim, Min-Young</au><au>Park, Chang Seo</au><au>Yoon, Tae-Jin</au><au>Seo, Young-Joon</au><au>Suhr, Ki-Beom</au><au>Park, Jang-Kyu</au><au>Lee, Jeung-Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-angiogenic effect of tetraacetyl-phytosphingosine</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2007-04</date><risdate>2007</risdate><volume>16</volume><issue>4</issue><spage>311</spage><epage>317</epage><pages>311-317</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>: In a search for the wound healing accelerators, we found that tetraacetyl‐phytosphingosine (TAPS), a sphingolipid metabolite produced by phytosphingosine acetylation, has significant inhibitory potential on healing of rabbit ear wound. As angiogenesis is fundamental to proper wound healing, we examined the effect of TAPS on angiogenesis using human umbilical vein endothelial cells cultured in vitro. TAPS markedly decreased vascular endothelial growth factor (VEGF)‐induced chemotactic migration and capillary‐like tube formation. Recognizing its inhibitory potential on angiogenesis, we further investigated the action mechanism of TAPS. TAPS significantly inhibited VEGF‐induced proteolytic enzyme production, including matrix metalloproteinase‐2, urokinase‐type plasminogen activator and plasminogen activator inhibitor‐1. TAPS also suppressed VEGF‐induced phosphorylation of p42/44 extracellular signal‐regulated kinase and c‐Jun N‐terminal kinase. In addition, TAPS abolished VEGF‐induced intracellular calcium increase, measured using laser scanning confocal microscopy. Together, these results suggest that TAPS exerts its inhibitory action on angiogenesis through the inhibition of mitogen‐activated protein kinase activation and intracellular calcium increase, thereby affecting the process of wound healing negatively.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17359337</pmid><doi>10.1111/j.1600-0625.2006.00530.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Acetylation angiogenesis Animals Biological and medical sciences calcium Calcium - metabolism Cell Movement Cells, Cultured Dermatology Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - enzymology Extracellular Signal-Regulated MAP Kinases - metabolism Humans JNK Mitogen-Activated Protein Kinases - metabolism Matrix Metalloproteinase 2 - metabolism Medical sciences mitogen-activated protein kinase Neovascularization, Physiologic - drug effects Phosphorylation - drug effects Plasminogen Activator Inhibitor 1 - metabolism proteolytic enzyme Rabbits Signal Transduction - drug effects Sphingosine - analogs & derivatives Sphingosine - chemistry Sphingosine - pharmacology tetraacetyl-phytosphingosine Umbilical Veins - cytology Urokinase-Type Plasminogen Activator - metabolism Vascular Endothelial Growth Factor A - pharmacology Wound Healing - drug effects |
| title | Anti-angiogenic effect of tetraacetyl-phytosphingosine |
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