Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell-induced by angiotensin II
Rhubarb, used as a traditional Chinese medicine for centuries, offers therapeutic potential for cardiovascular and other diseases. Emodin, extracted from the root extract of rhubarb has sparked increasing interest for therapeutic application. The main objective was to study the effect of emodin on c...
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| Veröffentlicht in: | Phytotherapy research 2008-02, Vol.22 (2), p.247-251 |
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| creator | Wang, ShiJun Liu, YunYing Fan, FangHua Yan, Jie Wang, XingXiang Chen, JunZhu |
| description | Rhubarb, used as a traditional Chinese medicine for centuries, offers therapeutic potential for cardiovascular and other diseases. Emodin, extracted from the root extract of rhubarb has sparked increasing interest for therapeutic application. The main objective was to study the effect of emodin on cultured vascular smooth muscle cells (VSMCs) proliferation induced by angiotensin II (Ang II) and the expression of proto-oncogene c-myc. VSMCs were cultured by the explant method, then incubated for 24, 48 and 72 h with emodin (10-80 μm) and Ang II, or were left untreated (control). Cell proliferation was measured by MTT assay and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), respectively. The expression of c-myc was measured by immunohistochemical staining and image analysis technique. Ang II increased the cell proliferation compared with the control group (p < 0.01). The expression of PCNA and c-myc was increased compared with the control group (p < 0.01). After pretreatment with emodin, the above indexes were obviously reduced compared with the Ang II group (p < 0.01). These findings suggested that emodin inhibited VSMCs proliferation induced by Ang II. Inhibition of the expression of c-myc might be correlated with the inhibitory effects. Copyright © 2007 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/ptr.2301 |
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Emodin, extracted from the root extract of rhubarb has sparked increasing interest for therapeutic application. The main objective was to study the effect of emodin on cultured vascular smooth muscle cells (VSMCs) proliferation induced by angiotensin II (Ang II) and the expression of proto-oncogene c-myc. VSMCs were cultured by the explant method, then incubated for 24, 48 and 72 h with emodin (10-80 μm) and Ang II, or were left untreated (control). Cell proliferation was measured by MTT assay and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), respectively. The expression of c-myc was measured by immunohistochemical staining and image analysis technique. Ang II increased the cell proliferation compared with the control group (p < 0.01). The expression of PCNA and c-myc was increased compared with the control group (p < 0.01). After pretreatment with emodin, the above indexes were obviously reduced compared with the Ang II group (p < 0.01). These findings suggested that emodin inhibited VSMCs proliferation induced by Ang II. Inhibition of the expression of c-myc might be correlated with the inhibitory effects. Copyright © 2007 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.2301</identifier><identifier>PMID: 17886230</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>angiotensin II ; Angiotensin II - pharmacology ; Animals ; Biological and medical sciences ; c-myc ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; emodin ; Emodin - chemistry ; Emodin - pharmacology ; General pharmacology ; Immunohistochemistry ; L-Lactate Dehydrogenase - metabolism ; Medical sciences ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; proliferating cell nuclear antigen ; Proliferating Cell Nuclear Antigen - metabolism ; Proto-Oncogene Proteins c-myc - metabolism ; Rats ; Rats, Sprague-Dawley ; Rheum - chemistry ; vascular smooth muscle cells</subject><ispartof>Phytotherapy research, 2008-02, Vol.22 (2), p.247-251</ispartof><rights>Copyright © 2007 John Wiley & Sons, Ltd.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4111-362d4681ef6462860711f962a717039f91916e7856aae03834a81bba5dd297a13</citedby><cites>FETCH-LOGICAL-c4111-362d4681ef6462860711f962a717039f91916e7856aae03834a81bba5dd297a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.2301$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.2301$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20126403$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17886230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, ShiJun</creatorcontrib><creatorcontrib>Liu, YunYing</creatorcontrib><creatorcontrib>Fan, FangHua</creatorcontrib><creatorcontrib>Yan, Jie</creatorcontrib><creatorcontrib>Wang, XingXiang</creatorcontrib><creatorcontrib>Chen, JunZhu</creatorcontrib><title>Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell-induced by angiotensin II</title><title>Phytotherapy research</title><addtitle>Phytother. Res</addtitle><description>Rhubarb, used as a traditional Chinese medicine for centuries, offers therapeutic potential for cardiovascular and other diseases. Emodin, extracted from the root extract of rhubarb has sparked increasing interest for therapeutic application. The main objective was to study the effect of emodin on cultured vascular smooth muscle cells (VSMCs) proliferation induced by angiotensin II (Ang II) and the expression of proto-oncogene c-myc. VSMCs were cultured by the explant method, then incubated for 24, 48 and 72 h with emodin (10-80 μm) and Ang II, or were left untreated (control). Cell proliferation was measured by MTT assay and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), respectively. The expression of c-myc was measured by immunohistochemical staining and image analysis technique. Ang II increased the cell proliferation compared with the control group (p < 0.01). The expression of PCNA and c-myc was increased compared with the control group (p < 0.01). After pretreatment with emodin, the above indexes were obviously reduced compared with the Ang II group (p < 0.01). These findings suggested that emodin inhibited VSMCs proliferation induced by Ang II. Inhibition of the expression of c-myc might be correlated with the inhibitory effects. Copyright © 2007 John Wiley & Sons, Ltd.</description><subject>angiotensin II</subject><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>c-myc</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>emodin</subject><subject>Emodin - chemistry</subject><subject>Emodin - pharmacology</subject><subject>General pharmacology</subject><subject>Immunohistochemistry</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Medical sciences</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>proliferating cell nuclear antigen</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rheum - chemistry</subject><subject>vascular smooth muscle cells</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10FFv1SAUB3BiXNx1mvgJlBeNL50caKE8LotuN9vUzC36RmgLu2hbrkCd99vLTZvtyScC_Djn8EfoFZBjIIR-2KZwTBmBJ2gFRMoCKsGeohWRFRQl1D8O0fMYfxJCJCXlM3QIoq55frBC9-tx4xqXfNhhY61pU8TeYjP4zo3YjzhtDN4G3ztrgk4un-TrdurTFEyH8xH-o2Pe64Dj4H3a4GGKbW9wa_q-cGM3tdk1O6zHO-eTGWOuu16_QAdW99G8XNYjdPvp483peXH55Wx9enJZtCUAFIzTruQ1GMtLTmtOBICVnGoBgjBpJUjgRtQV19oQVrNS19A0uuo6KoUGdoTezXXzH35PJiY1uLgfTY_GT1EJQrksCcvw_Qzb4GMMxqptcIMOOwVE7UNWOWS1DznT10vNqRlM9wiXVDN4u4Acje5t0GPr4oOjBCifexazu3e92f23ofp6c700XryLyfx98Dr8UlwwUanvn8-UEFf8HPi1usj-zeyt9krfhTzD7bfcnRFSVwIoZf8Atd6rEA</recordid><startdate>200802</startdate><enddate>200802</enddate><creator>Wang, ShiJun</creator><creator>Liu, YunYing</creator><creator>Fan, FangHua</creator><creator>Yan, Jie</creator><creator>Wang, XingXiang</creator><creator>Chen, JunZhu</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200802</creationdate><title>Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell-induced by angiotensin II</title><author>Wang, ShiJun ; Liu, YunYing ; Fan, FangHua ; Yan, Jie ; Wang, XingXiang ; Chen, JunZhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4111-362d4681ef6462860711f962a717039f91916e7856aae03834a81bba5dd297a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>angiotensin II</topic><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>c-myc</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>emodin</topic><topic>Emodin - chemistry</topic><topic>Emodin - pharmacology</topic><topic>General pharmacology</topic><topic>Immunohistochemistry</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Medical sciences</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>proliferating cell nuclear antigen</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>Proto-Oncogene Proteins c-myc - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rheum - chemistry</topic><topic>vascular smooth muscle cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, ShiJun</creatorcontrib><creatorcontrib>Liu, YunYing</creatorcontrib><creatorcontrib>Fan, FangHua</creatorcontrib><creatorcontrib>Yan, Jie</creatorcontrib><creatorcontrib>Wang, XingXiang</creatorcontrib><creatorcontrib>Chen, JunZhu</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, ShiJun</au><au>Liu, YunYing</au><au>Fan, FangHua</au><au>Yan, Jie</au><au>Wang, XingXiang</au><au>Chen, JunZhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell-induced by angiotensin II</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother. Res</addtitle><date>2008-02</date><risdate>2008</risdate><volume>22</volume><issue>2</issue><spage>247</spage><epage>251</epage><pages>247-251</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>Rhubarb, used as a traditional Chinese medicine for centuries, offers therapeutic potential for cardiovascular and other diseases. Emodin, extracted from the root extract of rhubarb has sparked increasing interest for therapeutic application. The main objective was to study the effect of emodin on cultured vascular smooth muscle cells (VSMCs) proliferation induced by angiotensin II (Ang II) and the expression of proto-oncogene c-myc. VSMCs were cultured by the explant method, then incubated for 24, 48 and 72 h with emodin (10-80 μm) and Ang II, or were left untreated (control). Cell proliferation was measured by MTT assay and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), respectively. The expression of c-myc was measured by immunohistochemical staining and image analysis technique. Ang II increased the cell proliferation compared with the control group (p < 0.01). The expression of PCNA and c-myc was increased compared with the control group (p < 0.01). After pretreatment with emodin, the above indexes were obviously reduced compared with the Ang II group (p < 0.01). These findings suggested that emodin inhibited VSMCs proliferation induced by Ang II. Inhibition of the expression of c-myc might be correlated with the inhibitory effects. Copyright © 2007 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>17886230</pmid><doi>10.1002/ptr.2301</doi><tpages>5</tpages></addata></record> |
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| subjects | angiotensin II Angiotensin II - pharmacology Animals Biological and medical sciences c-myc Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured emodin Emodin - chemistry Emodin - pharmacology General pharmacology Immunohistochemistry L-Lactate Dehydrogenase - metabolism Medical sciences Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments proliferating cell nuclear antigen Proliferating Cell Nuclear Antigen - metabolism Proto-Oncogene Proteins c-myc - metabolism Rats Rats, Sprague-Dawley Rheum - chemistry vascular smooth muscle cells |
| title | Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell-induced by angiotensin II |
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