Simultaneous Detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by Use of Molecular Beacons in a Duplex Real-Time PCR

A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with different fluorochromes. This duplex PCR, targeting the P1 adhesion gene for M. pneumonia...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-02, Vol.46 (2), p.727-731
Hauptverfasser:	Gullsby, Karolina, Storm, Martin, Bondeson, Kåre
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesins, Bacterial - genetics Bacterial Outer Membrane Proteins - genetics Bacteriology Biological and medical sciences Chlamydophila Infections - diagnosis Chlamydophila pneumoniae Chlamydophila pneumoniae - isolation & purification DNA Primers - genetics DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Humans MEDICIN MEDICINE Microbiology Miscellaneous Mycoplasma pneumoniae Mycoplasma pneumoniae - isolation & purification Pharynx - microbiology Pneumonia, Mycoplasma - diagnosis Polymerase Chain Reaction - methods Sensitivity and Specificity
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