Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source
We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. By using a 100 fs-pulsed Ti:Sapphire laser with a low-frequency chirp to...
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| Veröffentlicht in: | Journal of microscopy (Oxford) 2007-02, Vol.225 (2), p.126-136 |
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| creator | McCONNELL, G GIRKIN, J.M AMEER-BEG, S.M BARBER, P.R VOJNOVIC, B NG, T BANERJEE, A WATSON, T.F COOK, R.J |
| description | We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. By using a 100 fs-pulsed Ti:Sapphire laser with a low-frequency chirp to pump a 30-cm long section of photonic crystal fibre, a ps-pulsed white-light supercontinuum was created. Optical bandpass interference filters were then applied to this broad-bandwidth source to select the 488-nm excitation wavelength required to perform TCSPC FLIM of dental structures. Decayed dentine showed significantly shorter lifetimes, discriminating it from healthy tissue and hard, stained and thus affected but non-infected material. The white-light generation source provides a flexible method of producing variable-bandwidth visible and ps-pulsed light for TCSPC FLIM. The results from the dental tissue indicate a potential method of discriminating diseased tissue from sound, but stained tissue, which could be of crucial importance in limiting tissue resection during preparation for clinical restorations. |
| doi_str_mv | 10.1111/j.1365-2818.2007.01724.x |
| format | Article |
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By using a 100 fs-pulsed Ti:Sapphire laser with a low-frequency chirp to pump a 30-cm long section of photonic crystal fibre, a ps-pulsed white-light supercontinuum was created. Optical bandpass interference filters were then applied to this broad-bandwidth source to select the 488-nm excitation wavelength required to perform TCSPC FLIM of dental structures. Decayed dentine showed significantly shorter lifetimes, discriminating it from healthy tissue and hard, stained and thus affected but non-infected material. The white-light generation source provides a flexible method of producing variable-bandwidth visible and ps-pulsed light for TCSPC FLIM. The results from the dental tissue indicate a potential method of discriminating diseased tissue from sound, but stained tissue, which could be of crucial importance in limiting tissue resection during preparation for clinical restorations.</description><identifier>ISSN: 0022-2720</identifier><identifier>EISSN: 1365-2818</identifier><identifier>DOI: 10.1111/j.1365-2818.2007.01724.x</identifier><identifier>PMID: 17359247</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>42.65.-k nonlinear optics ; 87.64.Ni optical absorption ; Dental Caries - pathology ; Equipment Design ; fluorescence spectroscopy ; Humans ; magnetic circular dichroism ; Microscopy, Confocal - instrumentation ; Microscopy, Confocal - methods ; Microscopy, Fluorescence - instrumentation ; Microscopy, Fluorescence - methods ; Optics and Photonics - instrumentation ; PACS: 42.62.Be Biological and medical applications ; Tooth - anatomy & histology</subject><ispartof>Journal of microscopy (Oxford), 2007-02, Vol.225 (2), p.126-136</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4724-2815101c91098735996f9e3ed736e4f050977338523d794e9f37410c6d9b60143</citedby><cites>FETCH-LOGICAL-c4724-2815101c91098735996f9e3ed736e4f050977338523d794e9f37410c6d9b60143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2818.2007.01724.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2818.2007.01724.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17359247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McCONNELL, G</creatorcontrib><creatorcontrib>GIRKIN, J.M</creatorcontrib><creatorcontrib>AMEER-BEG, S.M</creatorcontrib><creatorcontrib>BARBER, P.R</creatorcontrib><creatorcontrib>VOJNOVIC, B</creatorcontrib><creatorcontrib>NG, T</creatorcontrib><creatorcontrib>BANERJEE, A</creatorcontrib><creatorcontrib>WATSON, T.F</creatorcontrib><creatorcontrib>COOK, R.J</creatorcontrib><title>Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source</title><title>Journal of microscopy (Oxford)</title><addtitle>J Microsc</addtitle><description>We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. 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The results from the dental tissue indicate a potential method of discriminating diseased tissue from sound, but stained tissue, which could be of crucial importance in limiting tissue resection during preparation for clinical restorations.</description><subject>42.65.-k nonlinear optics</subject><subject>87.64.Ni optical absorption</subject><subject>Dental Caries - pathology</subject><subject>Equipment Design</subject><subject>fluorescence spectroscopy</subject><subject>Humans</subject><subject>magnetic circular dichroism</subject><subject>Microscopy, Confocal - instrumentation</subject><subject>Microscopy, Confocal - methods</subject><subject>Microscopy, Fluorescence - instrumentation</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Optics and Photonics - instrumentation</subject><subject>PACS: 42.62.Be Biological and medical applications</subject><subject>Tooth - anatomy & histology</subject><issn>0022-2720</issn><issn>1365-2818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFcArdgnXP4mTBQtUFSgqYkG7tlLneiYjTzz4R9N5GN4VhxnBEryxde93ztX1IYQyqFk577Y1E21T8Y51NQdQNTDFZf34hKz-NJ6SFQDnFVccLsiLGLcA0DUdPCcXTImm51KtyM-7aYeV8SGgGxKONE7z2mG13_jkZ2p8nlOpUOuyDxgNzgapmyymoivt2XozODrthvWCeUtHNMOxGA1zMSvysVTmVJiYQjYpFxd6mNKGDvSwmRJWblpvEo15j6H4lWk57xZlMPiSPLODi_jqfF-S-4_Xd1efq9tvn26uPtxWRpa9l3UbBsz0DPpuWa1vbY8CRyValBYa6JUSomu4GFUvsbdCSQamHfuHFpgUl-TtyXcf_I-MMendVHZ1bpjR56gV8FYxDv8EOchWNqopYHcCTfAxBrR6H8onhaNmoJcM9VYvUeklKr1kqH9nqB-L9PV5Rn7Y4fhXeA6tAO9PwGFyePxvY_3l683yKvo3J70dvB7WYYr6_jsHJgrcyEb04hcP4LYM</recordid><startdate>200702</startdate><enddate>200702</enddate><creator>McCONNELL, G</creator><creator>GIRKIN, J.M</creator><creator>AMEER-BEG, S.M</creator><creator>BARBER, P.R</creator><creator>VOJNOVIC, B</creator><creator>NG, T</creator><creator>BANERJEE, A</creator><creator>WATSON, T.F</creator><creator>COOK, R.J</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200702</creationdate><title>Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source</title><author>McCONNELL, G ; GIRKIN, J.M ; AMEER-BEG, S.M ; BARBER, P.R ; VOJNOVIC, B ; NG, T ; BANERJEE, A ; WATSON, T.F ; COOK, R.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4724-2815101c91098735996f9e3ed736e4f050977338523d794e9f37410c6d9b60143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>42.65.-k nonlinear optics</topic><topic>87.64.Ni optical absorption</topic><topic>Dental Caries - pathology</topic><topic>Equipment Design</topic><topic>fluorescence spectroscopy</topic><topic>Humans</topic><topic>magnetic circular dichroism</topic><topic>Microscopy, Confocal - instrumentation</topic><topic>Microscopy, Confocal - methods</topic><topic>Microscopy, Fluorescence - instrumentation</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Optics and Photonics - instrumentation</topic><topic>PACS: 42.62.Be Biological and medical applications</topic><topic>Tooth - anatomy & histology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McCONNELL, G</creatorcontrib><creatorcontrib>GIRKIN, J.M</creatorcontrib><creatorcontrib>AMEER-BEG, S.M</creatorcontrib><creatorcontrib>BARBER, P.R</creatorcontrib><creatorcontrib>VOJNOVIC, B</creatorcontrib><creatorcontrib>NG, T</creatorcontrib><creatorcontrib>BANERJEE, A</creatorcontrib><creatorcontrib>WATSON, T.F</creatorcontrib><creatorcontrib>COOK, R.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microscopy (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McCONNELL, G</au><au>GIRKIN, J.M</au><au>AMEER-BEG, S.M</au><au>BARBER, P.R</au><au>VOJNOVIC, B</au><au>NG, T</au><au>BANERJEE, A</au><au>WATSON, T.F</au><au>COOK, R.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source</atitle><jtitle>Journal of microscopy (Oxford)</jtitle><addtitle>J Microsc</addtitle><date>2007-02</date><risdate>2007</risdate><volume>225</volume><issue>2</issue><spage>126</spage><epage>136</epage><pages>126-136</pages><issn>0022-2720</issn><eissn>1365-2818</eissn><abstract>We report the demonstration of time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex vivo decayed and healthy dentinal tooth structures, using a white-light supercontinuum excitation source. By using a 100 fs-pulsed Ti:Sapphire laser with a low-frequency chirp to pump a 30-cm long section of photonic crystal fibre, a ps-pulsed white-light supercontinuum was created. Optical bandpass interference filters were then applied to this broad-bandwidth source to select the 488-nm excitation wavelength required to perform TCSPC FLIM of dental structures. Decayed dentine showed significantly shorter lifetimes, discriminating it from healthy tissue and hard, stained and thus affected but non-infected material. The white-light generation source provides a flexible method of producing variable-bandwidth visible and ps-pulsed light for TCSPC FLIM. 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| subjects | 42.65.-k nonlinear optics 87.64.Ni optical absorption Dental Caries - pathology Equipment Design fluorescence spectroscopy Humans magnetic circular dichroism Microscopy, Confocal - instrumentation Microscopy, Confocal - methods Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Optics and Photonics - instrumentation PACS: 42.62.Be Biological and medical applications Tooth - anatomy & histology |
| title | Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source |
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