GENOME SIZE DETERMINATION IN CHAGAS DISEASE TRANSMITTING BUGS (HEMIPTERA-TRIATOMINAE) BY FLOW CYTOMETRY
Because information about genome size in triatomines is scarce and contradictory, we performed DNA quantification by flow cytometry in 13 species belonging to five genera (Dipetalogaster, Eratyrus, Panstrongylus, Rhodnius, and Triatoma) to infer overall tendencies and phylogenetic associations. The...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2007-03, Vol.76 (3), p.516-521 |
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| creator | PANZERA, FRANCISCO FERRANDIS, INES RAMSEY, JANINE SALAZAR-SCHETTINO, PAZ M CABRERA, MARGARITA MONROY, CARLOTA BARGUES, MARIA D MAS-COMA, SANTIAGO O'CONNOR, JOSE E ANGULO, VICTOR M JARAMILLO, NICOLAS PEREZ, RUBEN |
| description | Because information about genome size in triatomines is scarce and contradictory, we performed DNA quantification by flow cytometry in 13 species belonging to five genera (Dipetalogaster, Eratyrus, Panstrongylus, Rhodnius, and Triatoma) to infer overall tendencies and phylogenetic associations. The results show that the haploid DNA content of the subfamily Triatominae varies nearly 4-fold, from |
| doi_str_mv | 10.4269/ajtmh.2007.76.516 |
| format | Article |
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The results show that the haploid DNA content of the subfamily Triatominae varies nearly 4-fold, from<0.7 pg in Rhodnius species (0.6x10(9) bp) to 2.7 pg in Triatoma delpontei (2.6x10(9) bp). Considering that triatomines present similar chromosome numbers, we suggest that genome size differences are the result of variation in the quantity of repetitive DNA sequences localized in hetero and euchromatin. Changes in heterochromatin are particularly important when considering populations or closely related species; in more distant taxa, euchromatic changes also play a role. 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The results show that the haploid DNA content of the subfamily Triatominae varies nearly 4-fold, from<0.7 pg in Rhodnius species (0.6x10(9) bp) to 2.7 pg in Triatoma delpontei (2.6x10(9) bp). Considering that triatomines present similar chromosome numbers, we suggest that genome size differences are the result of variation in the quantity of repetitive DNA sequences localized in hetero and euchromatin. Changes in heterochromatin are particularly important when considering populations or closely related species; in more distant taxa, euchromatic changes also play a role. Our analyses indicate that flow cytometry is a useful tool for population, taxonomic, and evolutionary studies in this subfamily.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chagas Disease - transmission</subject><subject>DNA - analysis</subject><subject>Flow Cytometry - methods</subject><subject>Genome, Insect</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Insect Vectors - genetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Panstrongylus</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>Rhodnius</subject><subject>Triatoma</subject><subject>Triatominae</subject><subject>Triatominae - genetics</subject><subject>Trypanosomiasis</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFv0zAUhS0EYmXwA3hBfgGxh5RrO7aTx6zzUkttgppMqLxYbuasmZJ1xK2q_XuSrWKPPF3p6jvn6N6D0GcC05CK-Ie933fbKQWQUymmnIg3aEJCKQIiQv4WTQCABrFg8gx98P4egESUkPfojEgmIJJygu5SleVLhQv9W-ErVarVUmdJqfMM6wzP5kmaFPhKFyopFC5XSVYsdVnqLMWXN2mBv8_VUv8cVElQrnRS5qNaXeDLNb5e5L_wbD2sVLlaf0Tvatt69-k0z9HNtSpn82CRp3qWLIIqjON9EAkIo5rwWIj6tqo3hDEuGbU8Aum4i0Fu6IZzx2wlrasg5tEmgooCE8xG0rJz9O3F97Hf_Tk4vzdd4yvXtvbB7Q7eSKCCMUn_C5KYAYR8BMkLWPU773tXm8e-6Wz_ZAiYsQbzXIMZazBSmKGGQfPlZH7YdO72VXH6-wB8PQHWV7ate_tQNf6ViwTl403_uG1ztz02vTO-s2072BJzPB6HOPYc-BeLmZRg</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>PANZERA, FRANCISCO</creator><creator>FERRANDIS, INES</creator><creator>RAMSEY, JANINE</creator><creator>SALAZAR-SCHETTINO, PAZ M</creator><creator>CABRERA, MARGARITA</creator><creator>MONROY, CARLOTA</creator><creator>BARGUES, MARIA D</creator><creator>MAS-COMA, SANTIAGO</creator><creator>O'CONNOR, JOSE E</creator><creator>ANGULO, VICTOR M</creator><creator>JARAMILLO, NICOLAS</creator><creator>PEREZ, RUBEN</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>GENOME SIZE DETERMINATION IN CHAGAS DISEASE TRANSMITTING BUGS (HEMIPTERA-TRIATOMINAE) BY FLOW CYTOMETRY</title><author>PANZERA, FRANCISCO ; FERRANDIS, INES ; RAMSEY, JANINE ; SALAZAR-SCHETTINO, PAZ M ; CABRERA, MARGARITA ; MONROY, CARLOTA ; BARGUES, MARIA D ; MAS-COMA, SANTIAGO ; O'CONNOR, JOSE E ; ANGULO, VICTOR M ; JARAMILLO, NICOLAS ; PEREZ, RUBEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-86048f15966fdcfb1335732a5807e5e907b2b55e3ac7aec0958b80c20363a87a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chagas Disease - transmission</topic><topic>DNA - analysis</topic><topic>Flow Cytometry - methods</topic><topic>Genome, Insect</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Insect Vectors - genetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Panstrongylus</topic><topic>Parasitic diseases</topic><topic>Protozoal diseases</topic><topic>Rhodnius</topic><topic>Triatoma</topic><topic>Triatominae</topic><topic>Triatominae - genetics</topic><topic>Trypanosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PANZERA, FRANCISCO</creatorcontrib><creatorcontrib>FERRANDIS, INES</creatorcontrib><creatorcontrib>RAMSEY, JANINE</creatorcontrib><creatorcontrib>SALAZAR-SCHETTINO, PAZ M</creatorcontrib><creatorcontrib>CABRERA, MARGARITA</creatorcontrib><creatorcontrib>MONROY, CARLOTA</creatorcontrib><creatorcontrib>BARGUES, MARIA D</creatorcontrib><creatorcontrib>MAS-COMA, SANTIAGO</creatorcontrib><creatorcontrib>O'CONNOR, JOSE E</creatorcontrib><creatorcontrib>ANGULO, VICTOR M</creatorcontrib><creatorcontrib>JARAMILLO, NICOLAS</creatorcontrib><creatorcontrib>PEREZ, RUBEN</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PANZERA, FRANCISCO</au><au>FERRANDIS, INES</au><au>RAMSEY, JANINE</au><au>SALAZAR-SCHETTINO, PAZ M</au><au>CABRERA, MARGARITA</au><au>MONROY, CARLOTA</au><au>BARGUES, MARIA D</au><au>MAS-COMA, SANTIAGO</au><au>O'CONNOR, JOSE E</au><au>ANGULO, VICTOR M</au><au>JARAMILLO, NICOLAS</au><au>PEREZ, RUBEN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GENOME SIZE DETERMINATION IN CHAGAS DISEASE TRANSMITTING BUGS (HEMIPTERA-TRIATOMINAE) BY FLOW CYTOMETRY</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>76</volume><issue>3</issue><spage>516</spage><epage>521</epage><pages>516-521</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>Because information about genome size in triatomines is scarce and contradictory, we performed DNA quantification by flow cytometry in 13 species belonging to five genera (Dipetalogaster, Eratyrus, Panstrongylus, Rhodnius, and Triatoma) to infer overall tendencies and phylogenetic associations. The results show that the haploid DNA content of the subfamily Triatominae varies nearly 4-fold, from<0.7 pg in Rhodnius species (0.6x10(9) bp) to 2.7 pg in Triatoma delpontei (2.6x10(9) bp). Considering that triatomines present similar chromosome numbers, we suggest that genome size differences are the result of variation in the quantity of repetitive DNA sequences localized in hetero and euchromatin. Changes in heterochromatin are particularly important when considering populations or closely related species; in more distant taxa, euchromatic changes also play a role. Our analyses indicate that flow cytometry is a useful tool for population, taxonomic, and evolutionary studies in this subfamily.</abstract><cop>Lawrence, KS</cop><pub>ASTMH</pub><pmid>17360877</pmid><doi>10.4269/ajtmh.2007.76.516</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Chagas Disease - transmission DNA - analysis Flow Cytometry - methods Genome, Insect Human protozoal diseases Humans Infectious diseases Insect Vectors - genetics Male Medical sciences Panstrongylus Parasitic diseases Protozoal diseases Rhodnius Triatoma Triatominae Triatominae - genetics Trypanosomiasis |
| title | GENOME SIZE DETERMINATION IN CHAGAS DISEASE TRANSMITTING BUGS (HEMIPTERA-TRIATOMINAE) BY FLOW CYTOMETRY |
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