Mitochondria and apicoplast of Plasmodium falciparum: behaviour on subcellular fractionation and the implication
The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques--Percoll density gradient centrifugation and fluorescence-activated organelle...
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| Veröffentlicht in: | Mitochondrion 2007-02, Vol.7 (1-2), p.125-132 |
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| container_title | Mitochondrion |
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| creator | Kobayashi, Tamaki Sato, Shigeharu Takamiya, Shinzaburo Komaki-Yasuda, Kanako Yano, Kazuhiko Hirata, Ayami Onitsuka, Izumi Hata, Masayuki Mi-ichi, Fumika Tanaka, Takeshi Hase, Toshiharu Miyajima, Atsushi Kawazu, Shin-ichiro Watanabe, Yoh-ichi Kita, Kiyoshi |
| description | The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques--Percoll density gradient centrifugation and fluorescence-activated organelle sorting--for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium. |
| doi_str_mv | 10.1016/j.mito.2006.11.021 |
| format | Article |
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| subjects | Animals Cell Fractionation Centrifugation, Density Gradient Microscopy, Electron Mitochondria - ultrastructure Organelles - ultrastructure Plasmodium falciparum - ultrastructure |
| title | Mitochondria and apicoplast of Plasmodium falciparum: behaviour on subcellular fractionation and the implication |
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