Evaluation of commercial chromatographic adsorbents for the direct capture of polyclonal rabbit antibodies from clarified antiserum

We have carried out a rigorous evaluation of eight commercially available packed bed chromatography adsorbents for direct capture and purification of immunoglobulins from clarified rabbit antiserum. Three of these materials featured rProtein A (rProtein A Sepharose Fast Flow, Mabselect, Prosep rProt...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-03, Vol.848 (1), p.116-130
Hauptverfasser:	Bak, H., Thomas, O.R.T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Analysis Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Biological and medical sciences Biomimetic ligands Chromatography - instrumentation Chromatography - methods Chromatography, Affinity - methods Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology General pharmacology Hydrophobic charge induction Immune Sera - chemistry Immune Sera - immunology Immunoglobulin purification Immunoglobulins - chemistry Immunoglobulins - isolation & purification Ligands Lipoproteins Medical sciences Mixed mode chromatography Pharmacology. Drug treatments Porosity Protein A Protein Binding Rabbits
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