Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus
The paper describes the rapid and label-free detection of the white spot syndrome virus (WSSV) using a surface plasmon resonance (SPR) device based on gold films prepared by electroless plating. The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles...
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| Veröffentlicht in: | Biosensors & bioelectronics 2008-02, Vol.23 (7), p.1200-1207 |
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| creator | Lei, Yun Chen, Hongyu Dai, Heping Zeng, Zhaorui Lin, Yi Zhou, Feimeng Pang, Daiwen |
| description | The paper describes the rapid and label-free detection of the white spot syndrome virus (WSSV) using a surface plasmon resonance (SPR) device based on gold films prepared by electroless plating. The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles adsorbed on glass slides were characterized by transmission electron microscopy (TEM). Detection of the WSSV was performed through the binding between WSSV in solution and the anti-WSSV single chain variable fragment (scFv antibody) preimmobilized onto the sensor surface. Morphologies of the as-prepared gold films, gold films modified with self-assembled alkanethiol monolayers, and films covered with antibody were examined using an atomic force microscope (AFM). To demonstrate the viability of the method for real sample analysis, WSSV of different concentrations present in a shrimp hemolymph matrix was determined upon optimizing the surface density of the antibody molecules. The SPR device based on the electroless-plated gold films is capable of detecting concentration of WSSV as low as 2.5
ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays. |
| doi_str_mv | 10.1016/j.bios.2007.10.004 |
| format | Article |
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ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Electroless plating</subject><subject>Electroplating</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold - chemistry</subject><subject>Membranes, Artificial</subject><subject>Methods. Procedures. Technologies</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Surface plasmon resonance</subject><subject>Surface Plasmon Resonance - instrumentation</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Various methods and equipments</subject><subject>Viral Load - instrumentation</subject><subject>Viral Load - methods</subject><subject>White spot syndrome virus</subject><subject>White spot syndrome virus 1 - isolation & purification</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP0zAQhS0EYrsLf4AD8gVuKWMnTRyJC1otLNJKXOBsOc4YXDlx8SRF---ZqhXc4DSj52-erPeEeKVgq0C17_bbIWbaaoCOhS1A80RslOnqqtH17qnYQL9rq13b1lfimmgPDKoenosrZUDXqoONSHcJ_VJyQqLqkNyCo_ye0yhDTBPJkIsknCku8YiS1hKcR8kcTXmWBSnPbmZlxIVtIms5yF8_4sLwIS-SHuex5AnlMZaVXohnwSXCl5d5I759vPt6e189fPn0-fbDQ-Ubo5bKOOO074zu265TodF9XY994xDBqNHhwHuPBgZ-huB03YTBD33nAUZsTV3fiLdn30PJP1ekxU6RPKbkZswr2Q70zjS9_i-owfTQdi2D-gz6kokKBnsocXLl0SqwpzLs3p7KsKcyThqXwUevL-7rMOH49-SSPgNvLoAj71IonGWkPxx7Gc0JMPf-zCGHdoxYLPmInPsYC8duxxz_9Y_fj_6p3g</recordid><startdate>20080228</startdate><enddate>20080228</enddate><creator>Lei, Yun</creator><creator>Chen, Hongyu</creator><creator>Dai, Heping</creator><creator>Zeng, Zhaorui</creator><creator>Lin, Yi</creator><creator>Zhou, Feimeng</creator><creator>Pang, Daiwen</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080228</creationdate><title>Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus</title><author>Lei, Yun ; Chen, Hongyu ; Dai, Heping ; Zeng, Zhaorui ; Lin, Yi ; Zhou, Feimeng ; Pang, Daiwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-8a8a2c78296771f42933d94aee081daeb94a9e80b6770fa234fbcb97c00de6833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Electroless plating</topic><topic>Electroplating</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. 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The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles adsorbed on glass slides were characterized by transmission electron microscopy (TEM). Detection of the WSSV was performed through the binding between WSSV in solution and the anti-WSSV single chain variable fragment (scFv antibody) preimmobilized onto the sensor surface. Morphologies of the as-prepared gold films, gold films modified with self-assembled alkanethiol monolayers, and films covered with antibody were examined using an atomic force microscope (AFM). To demonstrate the viability of the method for real sample analysis, WSSV of different concentrations present in a shrimp hemolymph matrix was determined upon optimizing the surface density of the antibody molecules. The SPR device based on the electroless-plated gold films is capable of detecting concentration of WSSV as low as 2.5
ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>18023170</pmid><doi>10.1016/j.bios.2007.10.004</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Electroless plating Electroplating Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Gold - chemistry Membranes, Artificial Methods. Procedures. Technologies Reproducibility of Results Sensitivity and Specificity Surface plasmon resonance Surface Plasmon Resonance - instrumentation Surface Plasmon Resonance - methods Various methods and equipments Viral Load - instrumentation Viral Load - methods White spot syndrome virus White spot syndrome virus 1 - isolation & purification |
| title | Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus |
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