Role of uterine natural killer cells in angiogenesis of human decidua of the first-trimester pregnancy
Decidualization is accompanied by extensive angiogenesis, which is an essential step in the maturation of new blood vessels in mammalian pregnancy. The purpose of this study was to determine a distribution of uNK cells (CD56 + uNK or CD56 bright cells) in human decidua of the first-trimester pregnan...
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| Veröffentlicht in: | Science China. Life sciences 2008-02, Vol.51 (2), p.111-119 |
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| creator | Li, GuoHua Huang, Wei Xia, QingJie Yang, KaiXuan Liu, RaoFang Zhu, HuiLi Jiang, Wei |
| description | Decidualization is accompanied by extensive angiogenesis, which is an essential step in the maturation of new blood vessels in mammalian pregnancy. The purpose of this study was to determine a distribution of uNK cells (CD56
+
uNK or CD56
bright
cells) in human decidua of the first-trimester pregnancy, and investigate whether uNK cells in human decidua could express vascular endothelial growth factor (VEGF-A) and/or angiopoietin2 (Ang2). Our immunohistochemical staining results demonstrated that a great amount of uNK (CD56
+
) cells scattered throughout the decidual stroma and near endometrial gland and spiral vessels in human decidua. The protein expression of VEGF-A and Ang2 was detected in decidual stroma cells, capillary endothelial cells and glandular cells in tissue specimens. There was a positive correlation between microvessel density (MVD) and the number of the CD56-positive uNK cells in decidual stroma, and also between the number of the CD56-positive uNK cells and VEGF-A protein expression in the tissue. In addition, we found that uNK cells in human decidua could express
VEGF-A
mRNA, but not
Ang2
mRNA, in isolated uNK cells in human decidua of the first-trimester gestation by combination of LCM and Nested-PCR. Our study indicated that uNK cells, through expressing VEGF-A, may play an important role in the angiogenic response at the time of human decidualization and early placenta development. |
| doi_str_mv | 10.1007/s11427-008-0027-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70254744</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20696390</sourcerecordid><originalsourceid>FETCH-LOGICAL-c400t-3eb00e32eced5967eee35f643a89a88cf6240aec0c7062b5aa070e3a15b334a63</originalsourceid><addsrcrecordid>eNqFkU1rGzEQhkVoSNwkPyCXInrIbZvRx-rjWELSFgKFkpyFLM_aStdaV9o9-N9Hiw2GQulBzKB55h2NXkJuGXxhAPq-MCa5bgBMPTXRZ2TBjOIN19Z8qDmAaqyA9pJ8LOUNQNhWmgtyyQwX1hizIN2voUc6dHQaMceENPlxyr6nv2PfY6YB-77QmKhP6zisMWGJZeY309YnusIQV5OfL8YN0i7mMjZjjlssVY_uMq6TT2F_Tc473xe8OcYr8vr0-PLwvXn--e3Hw9fnJkiAsRG4BEDBMeCqtUojomg7JYU31hsTOsUleAwQNCi-bL0HXXnP2qUQ0itxRe4Ours8_JnqI9w2lnkHn3CYitPAW6ml_C_IQVklLFTw81_g2zDlVJdwnMtWawG2QuwAhTyUkrFzu_oHPu8dAzdb5Q5WuWqVm61yuvZ8OgpPyy2uTh1HbyrAD0CppbTGfJr8b9V3ZYGfDw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>224577309</pqid></control><display><type>article</type><title>Role of uterine natural killer cells in angiogenesis of human decidua of the first-trimester pregnancy</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><creator>Li, GuoHua ; Huang, Wei ; Xia, QingJie ; Yang, KaiXuan ; Liu, RaoFang ; Zhu, HuiLi ; Jiang, Wei</creator><creatorcontrib>Li, GuoHua ; Huang, Wei ; Xia, QingJie ; Yang, KaiXuan ; Liu, RaoFang ; Zhu, HuiLi ; Jiang, Wei</creatorcontrib><description>Decidualization is accompanied by extensive angiogenesis, which is an essential step in the maturation of new blood vessels in mammalian pregnancy. The purpose of this study was to determine a distribution of uNK cells (CD56
+
uNK or CD56
bright
cells) in human decidua of the first-trimester pregnancy, and investigate whether uNK cells in human decidua could express vascular endothelial growth factor (VEGF-A) and/or angiopoietin2 (Ang2). Our immunohistochemical staining results demonstrated that a great amount of uNK (CD56
+
) cells scattered throughout the decidual stroma and near endometrial gland and spiral vessels in human decidua. The protein expression of VEGF-A and Ang2 was detected in decidual stroma cells, capillary endothelial cells and glandular cells in tissue specimens. There was a positive correlation between microvessel density (MVD) and the number of the CD56-positive uNK cells in decidual stroma, and also between the number of the CD56-positive uNK cells and VEGF-A protein expression in the tissue. In addition, we found that uNK cells in human decidua could express
VEGF-A
mRNA, but not
Ang2
mRNA, in isolated uNK cells in human decidua of the first-trimester gestation by combination of LCM and Nested-PCR. Our study indicated that uNK cells, through expressing VEGF-A, may play an important role in the angiogenic response at the time of human decidualization and early placenta development.</description><identifier>ISSN: 1006-9305</identifier><identifier>ISSN: 1674-7305</identifier><identifier>EISSN: 1862-2798</identifier><identifier>EISSN: 1869-1889</identifier><identifier>DOI: 10.1007/s11427-008-0027-7</identifier><identifier>PMID: 18239888</identifier><language>eng</language><publisher>Beijing: Science in China Press</publisher><subject>Adult ; Biomedical and Life Sciences ; Decidua - blood supply ; Decidua - cytology ; Decidua - growth & development ; Decidua - immunology ; Female ; Humans ; Killer Cells, Natural - cytology ; Killer Cells, Natural - immunology ; Life Sciences ; Lymphocyte Count ; Microcirculation - cytology ; Microcirculation - immunology ; Neovascularization, Physiologic - immunology ; Pregnancy ; Pregnancy Trimester, First - immunology</subject><ispartof>Science China. Life sciences, 2008-02, Vol.51 (2), p.111-119</ispartof><rights>Science in China Press 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-3eb00e32eced5967eee35f643a89a88cf6240aec0c7062b5aa070e3a15b334a63</citedby><cites>FETCH-LOGICAL-c400t-3eb00e32eced5967eee35f643a89a88cf6240aec0c7062b5aa070e3a15b334a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18239888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, GuoHua</creatorcontrib><creatorcontrib>Huang, Wei</creatorcontrib><creatorcontrib>Xia, QingJie</creatorcontrib><creatorcontrib>Yang, KaiXuan</creatorcontrib><creatorcontrib>Liu, RaoFang</creatorcontrib><creatorcontrib>Zhu, HuiLi</creatorcontrib><creatorcontrib>Jiang, Wei</creatorcontrib><title>Role of uterine natural killer cells in angiogenesis of human decidua of the first-trimester pregnancy</title><title>Science China. Life sciences</title><addtitle>Sci. China Ser. C-Life Sci</addtitle><addtitle>Sci China C Life Sci</addtitle><description>Decidualization is accompanied by extensive angiogenesis, which is an essential step in the maturation of new blood vessels in mammalian pregnancy. The purpose of this study was to determine a distribution of uNK cells (CD56
+
uNK or CD56
bright
cells) in human decidua of the first-trimester pregnancy, and investigate whether uNK cells in human decidua could express vascular endothelial growth factor (VEGF-A) and/or angiopoietin2 (Ang2). Our immunohistochemical staining results demonstrated that a great amount of uNK (CD56
+
) cells scattered throughout the decidual stroma and near endometrial gland and spiral vessels in human decidua. The protein expression of VEGF-A and Ang2 was detected in decidual stroma cells, capillary endothelial cells and glandular cells in tissue specimens. There was a positive correlation between microvessel density (MVD) and the number of the CD56-positive uNK cells in decidual stroma, and also between the number of the CD56-positive uNK cells and VEGF-A protein expression in the tissue. In addition, we found that uNK cells in human decidua could express
VEGF-A
mRNA, but not
Ang2
mRNA, in isolated uNK cells in human decidua of the first-trimester gestation by combination of LCM and Nested-PCR. Our study indicated that uNK cells, through expressing VEGF-A, may play an important role in the angiogenic response at the time of human decidualization and early placenta development.</description><subject>Adult</subject><subject>Biomedical and Life Sciences</subject><subject>Decidua - blood supply</subject><subject>Decidua - cytology</subject><subject>Decidua - growth & development</subject><subject>Decidua - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Killer Cells, Natural - cytology</subject><subject>Killer Cells, Natural - immunology</subject><subject>Life Sciences</subject><subject>Lymphocyte Count</subject><subject>Microcirculation - cytology</subject><subject>Microcirculation - immunology</subject><subject>Neovascularization, Physiologic - immunology</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First - immunology</subject><issn>1006-9305</issn><issn>1674-7305</issn><issn>1862-2798</issn><issn>1869-1889</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1rGzEQhkVoSNwkPyCXInrIbZvRx-rjWELSFgKFkpyFLM_aStdaV9o9-N9Hiw2GQulBzKB55h2NXkJuGXxhAPq-MCa5bgBMPTXRZ2TBjOIN19Z8qDmAaqyA9pJ8LOUNQNhWmgtyyQwX1hizIN2voUc6dHQaMceENPlxyr6nv2PfY6YB-77QmKhP6zisMWGJZeY309YnusIQV5OfL8YN0i7mMjZjjlssVY_uMq6TT2F_Tc473xe8OcYr8vr0-PLwvXn--e3Hw9fnJkiAsRG4BEDBMeCqtUojomg7JYU31hsTOsUleAwQNCi-bL0HXXnP2qUQ0itxRe4Ours8_JnqI9w2lnkHn3CYitPAW6ml_C_IQVklLFTw81_g2zDlVJdwnMtWawG2QuwAhTyUkrFzu_oHPu8dAzdb5Q5WuWqVm61yuvZ8OgpPyy2uTh1HbyrAD0CppbTGfJr8b9V3ZYGfDw</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Li, GuoHua</creator><creator>Huang, Wei</creator><creator>Xia, QingJie</creator><creator>Yang, KaiXuan</creator><creator>Liu, RaoFang</creator><creator>Zhu, HuiLi</creator><creator>Jiang, Wei</creator><general>Science in China Press</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7T5</scope><scope>7X8</scope></search><sort><creationdate>20080201</creationdate><title>Role of uterine natural killer cells in angiogenesis of human decidua of the first-trimester pregnancy</title><author>Li, GuoHua ; Huang, Wei ; Xia, QingJie ; Yang, KaiXuan ; Liu, RaoFang ; Zhu, HuiLi ; Jiang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-3eb00e32eced5967eee35f643a89a88cf6240aec0c7062b5aa070e3a15b334a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Biomedical and Life Sciences</topic><topic>Decidua - blood supply</topic><topic>Decidua - cytology</topic><topic>Decidua - growth & development</topic><topic>Decidua - immunology</topic><topic>Female</topic><topic>Humans</topic><topic>Killer Cells, Natural - cytology</topic><topic>Killer Cells, Natural - immunology</topic><topic>Life Sciences</topic><topic>Lymphocyte Count</topic><topic>Microcirculation - cytology</topic><topic>Microcirculation - immunology</topic><topic>Neovascularization, Physiologic - immunology</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, GuoHua</creatorcontrib><creatorcontrib>Huang, Wei</creatorcontrib><creatorcontrib>Xia, QingJie</creatorcontrib><creatorcontrib>Yang, KaiXuan</creatorcontrib><creatorcontrib>Liu, RaoFang</creatorcontrib><creatorcontrib>Zhu, HuiLi</creatorcontrib><creatorcontrib>Jiang, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Immunology Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Science China. Life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, GuoHua</au><au>Huang, Wei</au><au>Xia, QingJie</au><au>Yang, KaiXuan</au><au>Liu, RaoFang</au><au>Zhu, HuiLi</au><au>Jiang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of uterine natural killer cells in angiogenesis of human decidua of the first-trimester pregnancy</atitle><jtitle>Science China. Life sciences</jtitle><stitle>Sci. China Ser. C-Life Sci</stitle><addtitle>Sci China C Life Sci</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>51</volume><issue>2</issue><spage>111</spage><epage>119</epage><pages>111-119</pages><issn>1006-9305</issn><issn>1674-7305</issn><eissn>1862-2798</eissn><eissn>1869-1889</eissn><abstract>Decidualization is accompanied by extensive angiogenesis, which is an essential step in the maturation of new blood vessels in mammalian pregnancy. The purpose of this study was to determine a distribution of uNK cells (CD56
+
uNK or CD56
bright
cells) in human decidua of the first-trimester pregnancy, and investigate whether uNK cells in human decidua could express vascular endothelial growth factor (VEGF-A) and/or angiopoietin2 (Ang2). Our immunohistochemical staining results demonstrated that a great amount of uNK (CD56
+
) cells scattered throughout the decidual stroma and near endometrial gland and spiral vessels in human decidua. The protein expression of VEGF-A and Ang2 was detected in decidual stroma cells, capillary endothelial cells and glandular cells in tissue specimens. There was a positive correlation between microvessel density (MVD) and the number of the CD56-positive uNK cells in decidual stroma, and also between the number of the CD56-positive uNK cells and VEGF-A protein expression in the tissue. In addition, we found that uNK cells in human decidua could express
VEGF-A
mRNA, but not
Ang2
mRNA, in isolated uNK cells in human decidua of the first-trimester gestation by combination of LCM and Nested-PCR. Our study indicated that uNK cells, through expressing VEGF-A, may play an important role in the angiogenic response at the time of human decidualization and early placenta development.</abstract><cop>Beijing</cop><pub>Science in China Press</pub><pmid>18239888</pmid><doi>10.1007/s11427-008-0027-7</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1006-9305 |
| ispartof | Science China. Life sciences, 2008-02, Vol.51 (2), p.111-119 |
| issn | 1006-9305 1674-7305 1862-2798 1869-1889 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals; Alma/SFX Local Collection |
| subjects | Adult Biomedical and Life Sciences Decidua - blood supply Decidua - cytology Decidua - growth & development Decidua - immunology Female Humans Killer Cells, Natural - cytology Killer Cells, Natural - immunology Life Sciences Lymphocyte Count Microcirculation - cytology Microcirculation - immunology Neovascularization, Physiologic - immunology Pregnancy Pregnancy Trimester, First - immunology |
| title | Role of uterine natural killer cells in angiogenesis of human decidua of the first-trimester pregnancy |
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