Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16 L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector
The optimized expression of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was...
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| Veröffentlicht in: | Protein expression and purification 2008-03, Vol.58 (1), p.154-161 |
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| creator | Čeřovská, Noemi Hoffmeisterová, Hana Pečenková, Tamara Moravec, Tomáš Synková, Helena Plchová, Helena Velemínský, Jiří |
| description | The optimized expression of recombinant
Potato virus A coat protein (ACP) carrying two different epitopes from
Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into
Potato X potexvirus (PVX) based vector and transiently expressed in plants using
Agrobacterium tumefaciens mediated inoculation.
To increase the level of expressed protein the transgenic
Nicotiana benthamiana plants expressing
Potato virus A HC-Pro gene and transgenic
Nicotiana tabacum, cv. Petit Havana SR1 carrying
Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and
Potato virus Y
O
(PVY
O) increased the expression of L2ACPE7 in
N. tabacum and in transgenic
N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only. |
| doi_str_mv | 10.1016/j.pep.2007.09.006 |
| format | Article |
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Potato virus A coat protein (ACP) carrying two different epitopes from
Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into
Potato X potexvirus (PVX) based vector and transiently expressed in plants using
Agrobacterium tumefaciens mediated inoculation.
To increase the level of expressed protein the transgenic
Nicotiana benthamiana plants expressing
Potato virus A HC-Pro gene and transgenic
Nicotiana tabacum, cv. Petit Havana SR1 carrying
Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and
Potato virus Y
O
(PVY
O) increased the expression of L2ACPE7 in
N. tabacum and in transgenic
N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only.</description><identifier>ISSN: 1046-5928</identifier><identifier>EISSN: 1096-0279</identifier><identifier>DOI: 10.1016/j.pep.2007.09.006</identifier><identifier>PMID: 17980618</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Agrobacterium tumefaciens ; Capsid Proteins - genetics ; Capsid Proteins - metabolism ; Epitopes expression ; Genetic Vectors ; Human papillomavirus ; Human papillomavirus 16 ; Humans ; Microscopy, Immunoelectron ; Nicotiana benthamiana ; Nicotiana tabacum ; Oncogene Proteins, Viral - genetics ; Oncogene Proteins, Viral - metabolism ; Papillomavirus E7 Proteins ; Peptide Fragments - genetics ; Peptide Fragments - isolation & purification ; Peptide Fragments - metabolism ; Plants, Genetically Modified ; Potato virus A ; Potato virus X ; Potato virus Y ; Potexvirus ; Potexvirus - genetics ; Potexvirus - physiology ; Potyvirus-like particles ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - isolation & purification ; Solanum tuberosum ; Synergistic infection ; Transient expression</subject><ispartof>Protein expression and purification, 2008-03, Vol.58 (1), p.154-161</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-1fb5ecbd197cfb281adfe2eaf7782f6dc8bf3419fe1a6b743c5041394e4155353</citedby><cites>FETCH-LOGICAL-c382t-1fb5ecbd197cfb281adfe2eaf7782f6dc8bf3419fe1a6b743c5041394e4155353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1046592807002215$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17980618$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Čeřovská, Noemi</creatorcontrib><creatorcontrib>Hoffmeisterová, Hana</creatorcontrib><creatorcontrib>Pečenková, Tamara</creatorcontrib><creatorcontrib>Moravec, Tomáš</creatorcontrib><creatorcontrib>Synková, Helena</creatorcontrib><creatorcontrib>Plchová, Helena</creatorcontrib><creatorcontrib>Velemínský, Jiří</creatorcontrib><title>Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16 L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector</title><title>Protein expression and purification</title><addtitle>Protein Expr Purif</addtitle><description>The optimized expression of recombinant
Potato virus A coat protein (ACP) carrying two different epitopes from
Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into
Potato X potexvirus (PVX) based vector and transiently expressed in plants using
Agrobacterium tumefaciens mediated inoculation.
To increase the level of expressed protein the transgenic
Nicotiana benthamiana plants expressing
Potato virus A HC-Pro gene and transgenic
Nicotiana tabacum, cv. Petit Havana SR1 carrying
Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and
Potato virus Y
O
(PVY
O) increased the expression of L2ACPE7 in
N. tabacum and in transgenic
N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only.</description><subject>Agrobacterium tumefaciens</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - metabolism</subject><subject>Epitopes expression</subject><subject>Genetic Vectors</subject><subject>Human papillomavirus</subject><subject>Human papillomavirus 16</subject><subject>Humans</subject><subject>Microscopy, Immunoelectron</subject><subject>Nicotiana benthamiana</subject><subject>Nicotiana tabacum</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Oncogene Proteins, Viral - metabolism</subject><subject>Papillomavirus E7 Proteins</subject><subject>Peptide Fragments - genetics</subject><subject>Peptide Fragments - isolation & purification</subject><subject>Peptide Fragments - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Potato virus A</subject><subject>Potato virus X</subject><subject>Potato virus Y</subject><subject>Potexvirus</subject><subject>Potexvirus - genetics</subject><subject>Potexvirus - physiology</subject><subject>Potyvirus-like particles</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Solanum tuberosum</subject><subject>Synergistic infection</subject><subject>Transient expression</subject><issn>1046-5928</issn><issn>1096-0279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEoqXwAFyQTwgOCR7HcWxxqqpCK63UHgriZjnOGLxk42AnK3rjHXgBno0nwdtdCXEpp5nDN_9I_1cUz4FWQEG8WVcTThWjtK2oqigVD4pjoEqUlLXq4W7nomwUk0fFk5TWlAII2jwujqBVkgqQx8Wvm2jG5HGcCX6fIqbkw0iCIxfXH0GQ85bkF7PvkbwyhnD--8dPQV8TM_YHYsX-IYDKjADLTM6xX_wGo7dkCvPt1scllYP_imQycfZ2wESW5MfP5DrMZg7kjiCfys4k7MkW7Rzi0-KRM0PCZ4d5Unx4d35zdlGurt5fnp2uSltLNpfgugZt14NqreuYBNM7ZGhc20rmRG9l52oOyiEY0bW8tg3lUCuOHJqmbuqT4uU-d4rh24Jp1hufLA6DGTEsSbeUNYwr9l8QlJCNkjyDsAdtDClFdHqKfmPirQaqd_70Wufq9M6fpkpnf_nmxSF86TbY_704CMvA2z2AuYutx6iTzfYs9j7mvnQf_D3xfwCIUKt1</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Čeřovská, Noemi</creator><creator>Hoffmeisterová, Hana</creator><creator>Pečenková, Tamara</creator><creator>Moravec, Tomáš</creator><creator>Synková, Helena</creator><creator>Plchová, Helena</creator><creator>Velemínský, Jiří</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080301</creationdate><title>Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16 L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector</title><author>Čeřovská, Noemi ; Hoffmeisterová, Hana ; Pečenková, Tamara ; Moravec, Tomáš ; Synková, Helena ; Plchová, Helena ; Velemínský, Jiří</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-1fb5ecbd197cfb281adfe2eaf7782f6dc8bf3419fe1a6b743c5041394e4155353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agrobacterium tumefaciens</topic><topic>Capsid Proteins - genetics</topic><topic>Capsid Proteins - metabolism</topic><topic>Epitopes expression</topic><topic>Genetic Vectors</topic><topic>Human papillomavirus</topic><topic>Human papillomavirus 16</topic><topic>Humans</topic><topic>Microscopy, Immunoelectron</topic><topic>Nicotiana benthamiana</topic><topic>Nicotiana tabacum</topic><topic>Oncogene Proteins, Viral - genetics</topic><topic>Oncogene Proteins, Viral - metabolism</topic><topic>Papillomavirus E7 Proteins</topic><topic>Peptide Fragments - genetics</topic><topic>Peptide Fragments - isolation & purification</topic><topic>Peptide Fragments - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Potato virus A</topic><topic>Potato virus X</topic><topic>Potato virus Y</topic><topic>Potexvirus</topic><topic>Potexvirus - genetics</topic><topic>Potexvirus - physiology</topic><topic>Potyvirus-like particles</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>Solanum tuberosum</topic><topic>Synergistic infection</topic><topic>Transient expression</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Čeřovská, Noemi</creatorcontrib><creatorcontrib>Hoffmeisterová, Hana</creatorcontrib><creatorcontrib>Pečenková, Tamara</creatorcontrib><creatorcontrib>Moravec, Tomáš</creatorcontrib><creatorcontrib>Synková, Helena</creatorcontrib><creatorcontrib>Plchová, Helena</creatorcontrib><creatorcontrib>Velemínský, Jiří</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Protein expression and purification</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Čeřovská, Noemi</au><au>Hoffmeisterová, Hana</au><au>Pečenková, Tamara</au><au>Moravec, Tomáš</au><au>Synková, Helena</au><au>Plchová, Helena</au><au>Velemínský, Jiří</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16 L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector</atitle><jtitle>Protein expression and purification</jtitle><addtitle>Protein Expr Purif</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>58</volume><issue>1</issue><spage>154</spage><epage>161</epage><pages>154-161</pages><issn>1046-5928</issn><eissn>1096-0279</eissn><abstract>The optimized expression of recombinant
Potato virus A coat protein (ACP) carrying two different epitopes from
Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into
Potato X potexvirus (PVX) based vector and transiently expressed in plants using
Agrobacterium tumefaciens mediated inoculation.
To increase the level of expressed protein the transgenic
Nicotiana benthamiana plants expressing
Potato virus A HC-Pro gene and transgenic
Nicotiana tabacum, cv. Petit Havana SR1 carrying
Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and
Potato virus Y
O
(PVY
O) increased the expression of L2ACPE7 in
N. tabacum and in transgenic
N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17980618</pmid><doi>10.1016/j.pep.2007.09.006</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-5928 |
| ispartof | Protein expression and purification, 2008-03, Vol.58 (1), p.154-161 |
| issn | 1046-5928 1096-0279 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70252492 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agrobacterium tumefaciens Capsid Proteins - genetics Capsid Proteins - metabolism Epitopes expression Genetic Vectors Human papillomavirus Human papillomavirus 16 Humans Microscopy, Immunoelectron Nicotiana benthamiana Nicotiana tabacum Oncogene Proteins, Viral - genetics Oncogene Proteins, Viral - metabolism Papillomavirus E7 Proteins Peptide Fragments - genetics Peptide Fragments - isolation & purification Peptide Fragments - metabolism Plants, Genetically Modified Potato virus A Potato virus X Potato virus Y Potexvirus Potexvirus - genetics Potexvirus - physiology Potyvirus-like particles Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - isolation & purification Solanum tuberosum Synergistic infection Transient expression |
| title | Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16 L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector |
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