Circulating Tumor Cells and Aberrant Methylation as Tumor Markers in Patients with Esophageal Cancer
Background: This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic approaches on blood samples from patients with esophageal squamous cell cancer. Materials and Methods: Preoperative blood samples were obtained from 44 esophageal cancer pat...
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| Veröffentlicht in: | Anticancer research 2007-01, Vol.27 (1B), p.535-539 |
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| creator | IKOMA, Daito ICHIKAWA, Daisuke UEDA, Yuji TANI, Nobuyuki TOMITA, Haruhisa SAI, Soujin KIKUCHI, Shojiro FUJIWARA, Hitoshi OTSUJI, Eigo YAMAGISHI, Hisakazu |
| description | Background: This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic
approaches on blood samples from patients with esophageal squamous cell cancer. Materials and Methods: Preoperative blood
samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase
chain reaction (RT-PCR) assay and methylation-specific polymerase chain reaction (MSP) assay for p16, E-cadherin and RARβ
genes. Results: Circulating tumor cells were detected in 12 patients (27%); 14 patients (32%) had aberrant methylation in
the promoter region of at least one gene (6, 4 and 4 patients, for p16, E-cadherin and RARβ, respectively). No abnormality
was detected by either assay in control plasmas. Altogether, 23 patients (53%) had a positive result in either molecular assay.
There was no correlation between either assay result and those of conventional serum markers. Conclusion: The RT-PCR and MSP
assays can serve as complementary markers for screening and monitoring esophageal cancer patients. |
| format | Article |
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approaches on blood samples from patients with esophageal squamous cell cancer. Materials and Methods: Preoperative blood
samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase
chain reaction (RT-PCR) assay and methylation-specific polymerase chain reaction (MSP) assay for p16, E-cadherin and RARβ
genes. Results: Circulating tumor cells were detected in 12 patients (27%); 14 patients (32%) had aberrant methylation in
the promoter region of at least one gene (6, 4 and 4 patients, for p16, E-cadherin and RARβ, respectively). No abnormality
was detected by either assay in control plasmas. Altogether, 23 patients (53%) had a positive result in either molecular assay.
There was no correlation between either assay result and those of conventional serum markers. Conclusion: The RT-PCR and MSP
assays can serve as complementary markers for screening and monitoring esophageal cancer patients.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 17348438</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Aged ; Biological and medical sciences ; Biomarkers, Tumor - blood ; Cadherins - genetics ; Carcinoembryonic Antigen - genetics ; Carcinoma, Squamous Cell - blood ; Carcinoma, Squamous Cell - diagnosis ; Carcinoma, Squamous Cell - genetics ; Cyclin-Dependent Kinase Inhibitor p16 - genetics ; DNA Methylation ; Esophageal Neoplasms - blood ; Esophageal Neoplasms - diagnosis ; Esophageal Neoplasms - genetics ; Esophagus ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Medical sciences ; Middle Aged ; Neoplastic Cells, Circulating - metabolism ; Neoplastic Cells, Circulating - pathology ; Polymerase Chain Reaction - methods ; Receptors, Retinoic Acid - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sensitivity and Specificity ; Tumors</subject><ispartof>Anticancer research, 2007-01, Vol.27 (1B), p.535-539</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18562215$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17348438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>IKOMA, Daito</creatorcontrib><creatorcontrib>ICHIKAWA, Daisuke</creatorcontrib><creatorcontrib>UEDA, Yuji</creatorcontrib><creatorcontrib>TANI, Nobuyuki</creatorcontrib><creatorcontrib>TOMITA, Haruhisa</creatorcontrib><creatorcontrib>SAI, Soujin</creatorcontrib><creatorcontrib>KIKUCHI, Shojiro</creatorcontrib><creatorcontrib>FUJIWARA, Hitoshi</creatorcontrib><creatorcontrib>OTSUJI, Eigo</creatorcontrib><creatorcontrib>YAMAGISHI, Hisakazu</creatorcontrib><title>Circulating Tumor Cells and Aberrant Methylation as Tumor Markers in Patients with Esophageal Cancer</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic
approaches on blood samples from patients with esophageal squamous cell cancer. Materials and Methods: Preoperative blood
samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase
chain reaction (RT-PCR) assay and methylation-specific polymerase chain reaction (MSP) assay for p16, E-cadherin and RARβ
genes. Results: Circulating tumor cells were detected in 12 patients (27%); 14 patients (32%) had aberrant methylation in
the promoter region of at least one gene (6, 4 and 4 patients, for p16, E-cadherin and RARβ, respectively). No abnormality
was detected by either assay in control plasmas. Altogether, 23 patients (53%) had a positive result in either molecular assay.
There was no correlation between either assay result and those of conventional serum markers. Conclusion: The RT-PCR and MSP
assays can serve as complementary markers for screening and monitoring esophageal cancer patients.</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - blood</subject><subject>Cadherins - genetics</subject><subject>Carcinoembryonic Antigen - genetics</subject><subject>Carcinoma, Squamous Cell - blood</subject><subject>Carcinoma, Squamous Cell - diagnosis</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - genetics</subject><subject>DNA Methylation</subject><subject>Esophageal Neoplasms - blood</subject><subject>Esophageal Neoplasms - diagnosis</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophagus</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplastic Cells, Circulating - metabolism</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Receptors, Retinoic Acid - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0F1LwzAUBuAgipvTvyC50btCmjRNcjnL_IANvZjXIU3TNZqmM2mR_XsjTnZ14JyHF857BuY5E3nGKEHnYI4wRRlDiM7AVYwfCJWl4OQSzHJGCl4QPgdNZYOenBqt38Ht1A8BVsa5CJVv4LI2ISg_wo0Zu8MvGjxU8eg2KnyaEKH18C2djB8j_LZjB1dx2HdqZ5SDlfLahGtw0SoXzc1xLsD742pbPWfr16eXarnOOlyKMTNl2yImMC8w0a0oqSBc1BrjVhRNaYQmWiOqalFgJnRDueB1URuUtqzMjSYLcP-Xuw_D12TiKHsbdXpHeTNMUbJUSC6ESPD2CKe6N43cB9urcJD_vSRwdwQqauXa1IK28eQ4LTHO6cl1dtd922Bk7JVzKZZIFTCT-YOkhJIfaiF5-g</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>IKOMA, Daito</creator><creator>ICHIKAWA, Daisuke</creator><creator>UEDA, Yuji</creator><creator>TANI, Nobuyuki</creator><creator>TOMITA, Haruhisa</creator><creator>SAI, Soujin</creator><creator>KIKUCHI, Shojiro</creator><creator>FUJIWARA, Hitoshi</creator><creator>OTSUJI, Eigo</creator><creator>YAMAGISHI, Hisakazu</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Circulating Tumor Cells and Aberrant Methylation as Tumor Markers in Patients with Esophageal Cancer</title><author>IKOMA, Daito ; ICHIKAWA, Daisuke ; UEDA, Yuji ; TANI, Nobuyuki ; TOMITA, Haruhisa ; SAI, Soujin ; KIKUCHI, Shojiro ; FUJIWARA, Hitoshi ; OTSUJI, Eigo ; YAMAGISHI, Hisakazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-e6ff07928423cf9659389bc22f94d6e9c3cc05ab94279cd5898b4be0cc0761ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - blood</topic><topic>Cadherins - genetics</topic><topic>Carcinoembryonic Antigen - genetics</topic><topic>Carcinoma, Squamous Cell - blood</topic><topic>Carcinoma, Squamous Cell - diagnosis</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - genetics</topic><topic>DNA Methylation</topic><topic>Esophageal Neoplasms - blood</topic><topic>Esophageal Neoplasms - diagnosis</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophagus</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplastic Cells, Circulating - metabolism</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Receptors, Retinoic Acid - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>IKOMA, Daito</creatorcontrib><creatorcontrib>ICHIKAWA, Daisuke</creatorcontrib><creatorcontrib>UEDA, Yuji</creatorcontrib><creatorcontrib>TANI, Nobuyuki</creatorcontrib><creatorcontrib>TOMITA, Haruhisa</creatorcontrib><creatorcontrib>SAI, Soujin</creatorcontrib><creatorcontrib>KIKUCHI, Shojiro</creatorcontrib><creatorcontrib>FUJIWARA, Hitoshi</creatorcontrib><creatorcontrib>OTSUJI, Eigo</creatorcontrib><creatorcontrib>YAMAGISHI, Hisakazu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>IKOMA, Daito</au><au>ICHIKAWA, Daisuke</au><au>UEDA, Yuji</au><au>TANI, Nobuyuki</au><au>TOMITA, Haruhisa</au><au>SAI, Soujin</au><au>KIKUCHI, Shojiro</au><au>FUJIWARA, Hitoshi</au><au>OTSUJI, Eigo</au><au>YAMAGISHI, Hisakazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating Tumor Cells and Aberrant Methylation as Tumor Markers in Patients with Esophageal Cancer</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>27</volume><issue>1B</issue><spage>535</spage><epage>539</epage><pages>535-539</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic
approaches on blood samples from patients with esophageal squamous cell cancer. Materials and Methods: Preoperative blood
samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase
chain reaction (RT-PCR) assay and methylation-specific polymerase chain reaction (MSP) assay for p16, E-cadherin and RARβ
genes. Results: Circulating tumor cells were detected in 12 patients (27%); 14 patients (32%) had aberrant methylation in
the promoter region of at least one gene (6, 4 and 4 patients, for p16, E-cadherin and RARβ, respectively). No abnormality
was detected by either assay in control plasmas. Altogether, 23 patients (53%) had a positive result in either molecular assay.
There was no correlation between either assay result and those of conventional serum markers. Conclusion: The RT-PCR and MSP
assays can serve as complementary markers for screening and monitoring esophageal cancer patients.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>17348438</pmid><tpages>5</tpages></addata></record> |
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| subjects | Aged Biological and medical sciences Biomarkers, Tumor - blood Cadherins - genetics Carcinoembryonic Antigen - genetics Carcinoma, Squamous Cell - blood Carcinoma, Squamous Cell - diagnosis Carcinoma, Squamous Cell - genetics Cyclin-Dependent Kinase Inhibitor p16 - genetics DNA Methylation Esophageal Neoplasms - blood Esophageal Neoplasms - diagnosis Esophageal Neoplasms - genetics Esophagus Female Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Regulation, Neoplastic Humans Male Medical sciences Middle Aged Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Polymerase Chain Reaction - methods Receptors, Retinoic Acid - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Sensitivity and Specificity Tumors |
| title | Circulating Tumor Cells and Aberrant Methylation as Tumor Markers in Patients with Esophageal Cancer |
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