Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells
Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targ...
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| Veröffentlicht in: | Antiviral research 2007-04, Vol.74 (1), p.77-81 |
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| creator | Ferreira, Helena Lage Spilki, Fernando Rosado Almeida, Renata Servan de Santos, Márcia M.A.B. Arns, Clarice Weis |
| description | Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy. |
| doi_str_mv | 10.1016/j.antiviral.2006.12.002 |
| format | Article |
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It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy.</description><identifier>ISSN: 0166-3542</identifier><identifier>EISSN: 1872-9096</identifier><identifier>DOI: 10.1016/j.antiviral.2006.12.002</identifier><identifier>PMID: 17275932</identifier><identifier>CODEN: ARSRDR</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiviral agents ; Avian metapneumovirus ; Biological and medical sciences ; Cell Line ; Chick Embryo ; Inhibition ; Medical sciences ; Metapneumovirus - genetics ; Metapneumovirus - growth & development ; Paramyxoviridae Infections - virology ; Pharmacology. Drug treatments ; Real time PCR ; RNA Interference ; RNA, Small Interfering - chemical synthesis ; RNA, Small Interfering - genetics ; RNA, Viral - chemical synthesis ; RNA, Viral - genetics ; Short interfering RNA ; Species Specificity ; Transfection - methods ; Viral Fusion Proteins - genetics ; Viral Proteins - genetics ; Virus Replication</subject><ispartof>Antiviral research, 2007-04, Vol.74 (1), p.77-81</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-a01874678af4229e4b387d104b205a43ad7d9bbedeb48e9816e37297a212dbd63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166354206003585$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18621606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17275932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferreira, Helena Lage</creatorcontrib><creatorcontrib>Spilki, Fernando Rosado</creatorcontrib><creatorcontrib>Almeida, Renata Servan de</creatorcontrib><creatorcontrib>Santos, Márcia M.A.B.</creatorcontrib><creatorcontrib>Arns, Clarice Weis</creatorcontrib><title>Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells</title><title>Antiviral research</title><addtitle>Antiviral Res</addtitle><description>Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy.</description><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiviral agents</subject><subject>Avian metapneumovirus</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chick Embryo</subject><subject>Inhibition</subject><subject>Medical sciences</subject><subject>Metapneumovirus - genetics</subject><subject>Metapneumovirus - growth & development</subject><subject>Paramyxoviridae Infections - virology</subject><subject>Pharmacology. Drug treatments</subject><subject>Real time PCR</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - chemical synthesis</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Viral - chemical synthesis</subject><subject>RNA, Viral - genetics</subject><subject>Short interfering RNA</subject><subject>Species Specificity</subject><subject>Transfection - methods</subject><subject>Viral Fusion Proteins - genetics</subject><subject>Viral Proteins - genetics</subject><subject>Virus Replication</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctu1DAUBmALgehQeAXwBtQuEnzJxPFyVHGpVApCwNby5WTwKHGmtjNSF7w7DjOiy668-ezzH_8IvaGkpoS273e1DtkffNRDzQhpa8pqQtgTtKKdYJUksn2KVkW2FV837Ay9SGlHChSye47OqGBiLTlboT_X4bc3Pvsp4KnH-uB1wCNkvQ8wj1OZMCd8sfny7dcljrAfvNX_rLnH32832IcMsYcIwQLOOm4h-7DFYbYDTPs4ZfABbyEAvri9LBrbechzBIctDEN6iZ71ekjw6nSeo58fP_y4-lzdfP10fbW5qWzDSa40KVs1reh03zAmoTG8E46SxjCy1g3XTjhpDDgwTQeyoy1wwaTQjDJnXMvP0bvjuyXS3Qwpq9GnJYEOMM1JCcKarmX0UUhlYZzxAsUR2jilFKFX--hHHe8VJWqpSO3U_4rUUpGiTJWKys3XpxGzGcE93Dt1UsDbE9DJ6qGPOlifHtwStCXLUpujg_JzBw9RJeuXIpyPYLNyk380zF_X0LSr</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Ferreira, Helena Lage</creator><creator>Spilki, Fernando Rosado</creator><creator>Almeida, Renata Servan de</creator><creator>Santos, Márcia M.A.B.</creator><creator>Arns, Clarice Weis</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells</title><author>Ferreira, Helena Lage ; Spilki, Fernando Rosado ; Almeida, Renata Servan de ; Santos, Márcia M.A.B. ; Arns, Clarice Weis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-a01874678af4229e4b387d104b205a43ad7d9bbedeb48e9816e37297a212dbd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiviral agents</topic><topic>Avian metapneumovirus</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chick Embryo</topic><topic>Inhibition</topic><topic>Medical sciences</topic><topic>Metapneumovirus - genetics</topic><topic>Metapneumovirus - growth & development</topic><topic>Paramyxoviridae Infections - virology</topic><topic>Pharmacology. 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It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17275932</pmid><doi>10.1016/j.antiviral.2006.12.002</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Avian metapneumovirus Biological and medical sciences Cell Line Chick Embryo Inhibition Medical sciences Metapneumovirus - genetics Metapneumovirus - growth & development Paramyxoviridae Infections - virology Pharmacology. Drug treatments Real time PCR RNA Interference RNA, Small Interfering - chemical synthesis RNA, Small Interfering - genetics RNA, Viral - chemical synthesis RNA, Viral - genetics Short interfering RNA Species Specificity Transfection - methods Viral Fusion Proteins - genetics Viral Proteins - genetics Virus Replication |
| title | Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells |
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