Electric Field-Enhanced Sensitivity of Grafted Ligands and Receptors
Particle-based agglutination tests consisting of receptors grafted to colloidal microparticles are useful for detecting small quantities of corresponding ligands of interest in fluid test samples, but detection limits of such tests are limited to a certain concentration and it is most desirable to l...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 2008-02, Vol.54 (2), p.366-370 |
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| creator | Negi, Ajay Singh Sood, Ajay Kumar |
| description | Particle-based agglutination tests consisting of receptors grafted to colloidal microparticles are useful for detecting small quantities of corresponding ligands of interest in fluid test samples, but detection limits of such tests are limited to a certain concentration and it is most desirable to lower the detection limits and to enhance the rate of recognition of ligands.
A mixture of receptor-coated colloidal microparticles and corresponding ligand was sandwiched between 2 indium tin oxide-coated glass plates. Electrohydrodynamic drag from an alternating-current electric field applied perpendicular to the plates increased the local concentration of the colloidal particles, improving the chances of ligand-receptor interaction and leading to the aggregation of the colloidal particles.
With this technique the sensitivity of the ligand-receptor recognition was increased by a factor as large as 50.
This method can improve the sensitivity of particle-based agglutination tests used in immunoassays and many other applications such as immunoprecipitation and chemical sniffing. |
| doi_str_mv | 10.1373/clinchem.2007.094417 |
| format | Article |
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A mixture of receptor-coated colloidal microparticles and corresponding ligand was sandwiched between 2 indium tin oxide-coated glass plates. Electrohydrodynamic drag from an alternating-current electric field applied perpendicular to the plates increased the local concentration of the colloidal particles, improving the chances of ligand-receptor interaction and leading to the aggregation of the colloidal particles.
With this technique the sensitivity of the ligand-receptor recognition was increased by a factor as large as 50.
This method can improve the sensitivity of particle-based agglutination tests used in immunoassays and many other applications such as immunoprecipitation and chemical sniffing.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2007.094417</identifier><identifier>PMID: 18156282</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Atoms & subatomic particles ; Binding sites ; Biological and medical sciences ; Biotin ; Chemicals ; Colloids ; Detection limits ; Electric fields ; Electromagnetic Fields ; Environmental science ; Fundamental and applied biological sciences. Psychology ; Glass ; Immunoassays ; Indium tin oxides ; Investigative techniques, diagnostic techniques (general aspects) ; Latex Fixation Tests - methods ; Ligands ; Magnetic fields ; Medical sciences ; Methods ; Particle Size ; Reaction kinetics ; Reagent Kits, Diagnostic ; Rheumatoid Factor - analysis ; Ribonuclease, Pancreatic - analysis ; Sensitivity and Specificity ; Streptavidin ; Tin Compounds</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2008-02, Vol.54 (2), p.366-370</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright American Association for Clinical Chemistry Feb 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-1b1ba43fe048a75ad046c9c47a0334959ebd6c382f200cd51f46c905ae0a45323</citedby><cites>FETCH-LOGICAL-c438t-1b1ba43fe048a75ad046c9c47a0334959ebd6c382f200cd51f46c905ae0a45323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20053340$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18156282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Negi, Ajay Singh</creatorcontrib><creatorcontrib>Sood, Ajay Kumar</creatorcontrib><title>Electric Field-Enhanced Sensitivity of Grafted Ligands and Receptors</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>Particle-based agglutination tests consisting of receptors grafted to colloidal microparticles are useful for detecting small quantities of corresponding ligands of interest in fluid test samples, but detection limits of such tests are limited to a certain concentration and it is most desirable to lower the detection limits and to enhance the rate of recognition of ligands.
A mixture of receptor-coated colloidal microparticles and corresponding ligand was sandwiched between 2 indium tin oxide-coated glass plates. Electrohydrodynamic drag from an alternating-current electric field applied perpendicular to the plates increased the local concentration of the colloidal particles, improving the chances of ligand-receptor interaction and leading to the aggregation of the colloidal particles.
With this technique the sensitivity of the ligand-receptor recognition was increased by a factor as large as 50.
This method can improve the sensitivity of particle-based agglutination tests used in immunoassays and many other applications such as immunoprecipitation and chemical sniffing.</description><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Atoms & subatomic particles</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Biotin</subject><subject>Chemicals</subject><subject>Colloids</subject><subject>Detection limits</subject><subject>Electric fields</subject><subject>Electromagnetic Fields</subject><subject>Environmental science</subject><subject>Fundamental and applied biological sciences. 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A mixture of receptor-coated colloidal microparticles and corresponding ligand was sandwiched between 2 indium tin oxide-coated glass plates. Electrohydrodynamic drag from an alternating-current electric field applied perpendicular to the plates increased the local concentration of the colloidal particles, improving the chances of ligand-receptor interaction and leading to the aggregation of the colloidal particles.
With this technique the sensitivity of the ligand-receptor recognition was increased by a factor as large as 50.
This method can improve the sensitivity of particle-based agglutination tests used in immunoassays and many other applications such as immunoprecipitation and chemical sniffing.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>18156282</pmid><doi>10.1373/clinchem.2007.094417</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0009-9147 |
| ispartof | Clinical chemistry (Baltimore, Md.), 2008-02, Vol.54 (2), p.366-370 |
| issn | 0009-9147 1530-8561 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70248489 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Analytical chemistry Analytical, structural and metabolic biochemistry Atoms & subatomic particles Binding sites Biological and medical sciences Biotin Chemicals Colloids Detection limits Electric fields Electromagnetic Fields Environmental science Fundamental and applied biological sciences. Psychology Glass Immunoassays Indium tin oxides Investigative techniques, diagnostic techniques (general aspects) Latex Fixation Tests - methods Ligands Magnetic fields Medical sciences Methods Particle Size Reaction kinetics Reagent Kits, Diagnostic Rheumatoid Factor - analysis Ribonuclease, Pancreatic - analysis Sensitivity and Specificity Streptavidin Tin Compounds |
| title | Electric Field-Enhanced Sensitivity of Grafted Ligands and Receptors |
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