Novel “Lipid-Flow Chip” Configuration to Determine Donor-to-Acceptor Ratio-Dependent Fluorescence Resonance Energy Transfer Efficiency

We report on the determination of fluorescence resonance energy transfer (FRET) efficiency, which is dependent on the donor-to-acceptor (D−A) ratio, by using a new type of microchannel device called a “lipid-flow chip”. The chip comprises two supported lipid bilayers (SLBs) that self-spread from eit...

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Veröffentlicht in:	Langmuir 2008-02, Vol.24 (3), p.921-926
Hauptverfasser:	Furukawa, Kazuaki, Nakashima, Hiroshi, Kashimura, Yoshiaki, Torimitsu, Keiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry Colloidal state and disperse state Diffusion Exact sciences and technology Fluorescence Resonance Energy Transfer - instrumentation Fluorescence Resonance Energy Transfer - methods Fluorescence Resonance Energy Transfer - statistics & numerical data Fluorescent Dyes General and physical chemistry Lipid Bilayers - chemistry Microfluidic Analytical Techniques Microscopy, Confocal Oxadiazoles Surface physical chemistry Xanthenes
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