A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach
The efficiency of a monomolecular film of (3-glycidoxypropyl) trimethoxysilane (GPTS) on a shear horizontal guided (Love) acoustic wave immunosensor to detect whole Escherichia coli ( E. coli) bacteria is demonstrated. Direct anti- E. coli antibodies grafting onto the sensor surface did not lead to...
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| Veröffentlicht in: | Biosensors & bioelectronics 2007-04, Vol.22 (9), p.2145-2150 |
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| creator | Moll, Nicolas Pascal, Emilie Dinh, Duy Haï Pillot, Jean-Paul Bennetau, Bernard Rebière, Dominique Moynet, Daniel Mas, Yan Mossalayi, Djavad Pistré, Jacques Déjous, Corinne |
| description | The efficiency of a monomolecular film of (3-glycidoxypropyl) trimethoxysilane (GPTS) on a shear horizontal guided (Love) acoustic wave immunosensor to detect whole
Escherichia coli (
E. coli) bacteria is demonstrated. Direct anti-
E. coli antibodies grafting onto the sensor surface did not lead to a significant bacteria immobilisation, partially attributed to the SiO
2 sensor surface roughness. An innovative method has been set up to get around this difficulty and to detect whole bacteria. It consists in grafting goat anti-mouse antibodies (GAM) onto the sensor surface in a first step and introducing
E. coli bacteria mixed with anti-
E. coli antibodies onto the sensor in a second step. We describe the characteristics of such a technique like sample preparation time (lower than 30
min) and temperature improvements. A 37
°C experimental temperature led to the fastest bacteria binding kinetic, reducing the total analysis time. This method enables to keep the specificity of the antibody/antigen interaction and provides significant results in less than 1
h. This leads to a detection threshold of 10
6 bacteria/ml in a 500
μl chamber. |
| doi_str_mv | 10.1016/j.bios.2006.09.032 |
| format | Article |
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Escherichia coli (
E. coli) bacteria is demonstrated. Direct anti-
E. coli antibodies grafting onto the sensor surface did not lead to a significant bacteria immobilisation, partially attributed to the SiO
2 sensor surface roughness. An innovative method has been set up to get around this difficulty and to detect whole bacteria. It consists in grafting goat anti-mouse antibodies (GAM) onto the sensor surface in a first step and introducing
E. coli bacteria mixed with anti-
E. coli antibodies onto the sensor in a second step. We describe the characteristics of such a technique like sample preparation time (lower than 30
min) and temperature improvements. A 37
°C experimental temperature led to the fastest bacteria binding kinetic, reducing the total analysis time. This method enables to keep the specificity of the antibody/antigen interaction and provides significant results in less than 1
h. This leads to a detection threshold of 10
6 bacteria/ml in a 500
μl chamber.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2006.09.032</identifier><identifier>PMID: 17097870</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; Escherichia coli ; Escherichia coli - immunology ; Escherichia coli - isolation & purification ; Fundamental and applied biological sciences. Psychology ; GPTS ; Immunoassay - instrumentation ; Immunodetection ; Love wave sensors ; Mice ; Monomolecular film</subject><ispartof>Biosensors & bioelectronics, 2007-04, Vol.22 (9), p.2145-2150</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-863e18aab298ff4ff9aaa67c2c26196d569cfaa42ea1cd89d17ffd4cc8ce7d2e3</citedby><cites>FETCH-LOGICAL-c415t-863e18aab298ff4ff9aaa67c2c26196d569cfaa42ea1cd89d17ffd4cc8ce7d2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2006.09.032$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,778,782,787,788,3539,23913,23914,25123,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18658205$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17097870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moll, Nicolas</creatorcontrib><creatorcontrib>Pascal, Emilie</creatorcontrib><creatorcontrib>Dinh, Duy Haï</creatorcontrib><creatorcontrib>Pillot, Jean-Paul</creatorcontrib><creatorcontrib>Bennetau, Bernard</creatorcontrib><creatorcontrib>Rebière, Dominique</creatorcontrib><creatorcontrib>Moynet, Daniel</creatorcontrib><creatorcontrib>Mas, Yan</creatorcontrib><creatorcontrib>Mossalayi, Djavad</creatorcontrib><creatorcontrib>Pistré, Jacques</creatorcontrib><creatorcontrib>Déjous, Corinne</creatorcontrib><title>A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>The efficiency of a monomolecular film of (3-glycidoxypropyl) trimethoxysilane (GPTS) on a shear horizontal guided (Love) acoustic wave immunosensor to detect whole
Escherichia coli (
E. coli) bacteria is demonstrated. Direct anti-
E. coli antibodies grafting onto the sensor surface did not lead to a significant bacteria immobilisation, partially attributed to the SiO
2 sensor surface roughness. An innovative method has been set up to get around this difficulty and to detect whole bacteria. It consists in grafting goat anti-mouse antibodies (GAM) onto the sensor surface in a first step and introducing
E. coli bacteria mixed with anti-
E. coli antibodies onto the sensor in a second step. We describe the characteristics of such a technique like sample preparation time (lower than 30
min) and temperature improvements. A 37
°C experimental temperature led to the fastest bacteria binding kinetic, reducing the total analysis time. This method enables to keep the specificity of the antibody/antigen interaction and provides significant results in less than 1
h. This leads to a detection threshold of 10
6 bacteria/ml in a 500
μl chamber.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GPTS</subject><subject>Immunoassay - instrumentation</subject><subject>Immunodetection</subject><subject>Love wave sensors</subject><subject>Mice</subject><subject>Monomolecular film</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EotPCC7BA2cAuwXYS_0hsqqpQpJHYwNq6ca6pR4k92MmM-vZ4mJG6g8X13Zzvs-VDyDtGG0aZ-LRrBh9zwykVDdUNbfkLsmFKtnXH2_4l2VDdi7oXor0i1znvKKWSafqaXDFJtVSSbki6rbbxgNURyuHneQ0xY8gxVa7M8TFOWN03lY2TrwawCyYP1YgL2sXHUK3Zh18VhMqHEA-w-NKyHGOdF9yf6uLgJ5_hLwv7fYpgH9-QVw6mjG8v-4b8_HL_4-6h3n7_-u3udlvbjvVLrUSLTAEMXCvnOuc0AAhpueWCaTH2QlsH0HEEZkelRyadGztrlUU5cmxvyMdzb7n294p5MbPPFqcJAsY1G0l5J2TX_hfkVHLFBS0gP4M2xZwTOrNPfob0ZBg1JyVmZ05KzEmJodoUJSX0_tK-DjOOz5GLgwJ8uACQLUwuQbA-P3NK9IrTvnCfzxyWTzt4TCZbj8Hi6FPRYcbo__WOP2fCrOE</recordid><startdate>20070415</startdate><enddate>20070415</enddate><creator>Moll, Nicolas</creator><creator>Pascal, Emilie</creator><creator>Dinh, Duy Haï</creator><creator>Pillot, Jean-Paul</creator><creator>Bennetau, Bernard</creator><creator>Rebière, Dominique</creator><creator>Moynet, Daniel</creator><creator>Mas, Yan</creator><creator>Mossalayi, Djavad</creator><creator>Pistré, Jacques</creator><creator>Déjous, Corinne</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070415</creationdate><title>A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach</title><author>Moll, Nicolas ; Pascal, Emilie ; Dinh, Duy Haï ; Pillot, Jean-Paul ; Bennetau, Bernard ; Rebière, Dominique ; Moynet, Daniel ; Mas, Yan ; Mossalayi, Djavad ; Pistré, Jacques ; Déjous, Corinne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-863e18aab298ff4ff9aaa67c2c26196d569cfaa42ea1cd89d17ffd4cc8ce7d2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - isolation & purification</topic><topic>Fundamental and applied biological sciences. 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Escherichia coli (
E. coli) bacteria is demonstrated. Direct anti-
E. coli antibodies grafting onto the sensor surface did not lead to a significant bacteria immobilisation, partially attributed to the SiO
2 sensor surface roughness. An innovative method has been set up to get around this difficulty and to detect whole bacteria. It consists in grafting goat anti-mouse antibodies (GAM) onto the sensor surface in a first step and introducing
E. coli bacteria mixed with anti-
E. coli antibodies onto the sensor in a second step. We describe the characteristics of such a technique like sample preparation time (lower than 30
min) and temperature improvements. A 37
°C experimental temperature led to the fastest bacteria binding kinetic, reducing the total analysis time. This method enables to keep the specificity of the antibody/antigen interaction and provides significant results in less than 1
h. This leads to a detection threshold of 10
6 bacteria/ml in a 500
μl chamber.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>17097870</pmid><doi>10.1016/j.bios.2006.09.032</doi><tpages>6</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Escherichia coli Escherichia coli - immunology Escherichia coli - isolation & purification Fundamental and applied biological sciences. Psychology GPTS Immunoassay - instrumentation Immunodetection Love wave sensors Mice Monomolecular film |
| title | A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach |
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