Characterisation of plasmids encoding CTX-M-3 extended-spectrum β-lactamase from Enterobacteriaceae isolated at a university hospital in Taiwan
Abstract CTX-M-3 is the most common extended-spectrum β-lactamase produced by Enterobacteriaceae in Taiwan. The present study was conducted to characterise the genetic environment surrounding blaCTX-M-3 . A total of 11 ceftriaxone-resistant isolates were studied: Escherichia coli ( n = 4), Klebsiell...
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| Veröffentlicht in: | International journal of antimicrobial agents 2007-04, Vol.29 (4), p.440-445 |
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| creator | Liu, S.Y Su, L.H Yeh, Y.L Chu, C Lai, J.C Chiu, C.H |
| description | Abstract CTX-M-3 is the most common extended-spectrum β-lactamase produced by Enterobacteriaceae in Taiwan. The present study was conducted to characterise the genetic environment surrounding blaCTX-M-3 . A total of 11 ceftriaxone-resistant isolates were studied: Escherichia coli ( n = 4), Klebsiella pneumoniae ( n = 5) and Salmonella enterica serotypes Anatum (SA831R) and Potsdam (SC72). Molecular methods used included polymerase chain reaction, sequencing, DNA–DNA hybridisation, conjugation, physical mapping and restriction fragment length polymorphism (RFLP) analysis. All isolates examined carried blaCTX-M-3 on large plasmids (>70 kb). The resistance plasmids of the two Salmonella and two K. pneumoniae strains (KP104 and KP116) were confirmed to be conjugative in vitro. RFLP analysis indicated that the plasmids were different. Physical mapping also revealed the difference between the two Salmonella plasmids, pSA831R (82 kb) and pSC72 (74 kb). An insertion sequence, IS Ecp1 , was found upstream of each blaCTX-M-3 gene. However, sequencing of downstream regions of the bla genes showed two different patterns: the presence of orf477 in pSA831R and of orf1 - mucA in pSC72, pKP104 and pKP116. IncI1-type oriT and nikA sequences were present in the plasmids of all the clinical isolates tested, except S. Anatum. Different blaCTX-M-3 -carrying plasmids were identified among the enterobacteria studied. The presence of IS Ecp1 in all isolates may be associated with the widespread resistance among Enterobacteriaceae. Although the plasmids were not identical, they appeared to belong to the same incompatibility group (IncI1-like plasmids), suggesting that they are genetically related but may have evolved divergently over time. |
| doi_str_mv | 10.1016/j.ijantimicag.2006.11.013 |
| format | Article |
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The present study was conducted to characterise the genetic environment surrounding blaCTX-M-3 . A total of 11 ceftriaxone-resistant isolates were studied: Escherichia coli ( n = 4), Klebsiella pneumoniae ( n = 5) and Salmonella enterica serotypes Anatum (SA831R) and Potsdam (SC72). Molecular methods used included polymerase chain reaction, sequencing, DNA–DNA hybridisation, conjugation, physical mapping and restriction fragment length polymorphism (RFLP) analysis. All isolates examined carried blaCTX-M-3 on large plasmids (>70 kb). The resistance plasmids of the two Salmonella and two K. pneumoniae strains (KP104 and KP116) were confirmed to be conjugative in vitro. RFLP analysis indicated that the plasmids were different. Physical mapping also revealed the difference between the two Salmonella plasmids, pSA831R (82 kb) and pSC72 (74 kb). An insertion sequence, IS Ecp1 , was found upstream of each blaCTX-M-3 gene. However, sequencing of downstream regions of the bla genes showed two different patterns: the presence of orf477 in pSA831R and of orf1 - mucA in pSC72, pKP104 and pKP116. IncI1-type oriT and nikA sequences were present in the plasmids of all the clinical isolates tested, except S. Anatum. Different blaCTX-M-3 -carrying plasmids were identified among the enterobacteria studied. The presence of IS Ecp1 in all isolates may be associated with the widespread resistance among Enterobacteriaceae. Although the plasmids were not identical, they appeared to belong to the same incompatibility group (IncI1-like plasmids), suggesting that they are genetically related but may have evolved divergently over time.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2006.11.013</identifier><identifier>PMID: 17275265</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antimicrobial resistance ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; Biological and medical sciences ; Ceftriaxone - pharmacology ; Conjugation ; Conjugation, Genetic ; DNA Transposable Elements ; Drug Resistance, Bacterial - genetics ; Enterobacteriaceae ; Enterobacteriaceae - drug effects ; Enterobacteriaceae - genetics ; Enterobacteriaceae - isolation & purification ; Enterobacteriaceae Infections - microbiology ; Escherichia coli ; Hospitals, University ; Humans ; Infectious Disease ; Klebsiella pneumoniae ; Medical sciences ; Pharmacology. Drug treatments ; Plasmids - genetics ; Restriction Mapping ; Salmonella enterica ; Taiwan</subject><ispartof>International journal of antimicrobial agents, 2007-04, Vol.29 (4), p.440-445</ispartof><rights>Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2006 Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-ca64d1ac80af4c17c4e62a586e7fe708a9b90a5ae9bc0beeb845dd6d11aa76043</citedby><cites>FETCH-LOGICAL-c491t-ca64d1ac80af4c17c4e62a586e7fe708a9b90a5ae9bc0beeb845dd6d11aa76043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijantimicag.2006.11.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18617109$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17275265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, S.Y</creatorcontrib><creatorcontrib>Su, L.H</creatorcontrib><creatorcontrib>Yeh, Y.L</creatorcontrib><creatorcontrib>Chu, C</creatorcontrib><creatorcontrib>Lai, J.C</creatorcontrib><creatorcontrib>Chiu, C.H</creatorcontrib><title>Characterisation of plasmids encoding CTX-M-3 extended-spectrum β-lactamase from Enterobacteriaceae isolated at a university hospital in Taiwan</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>Abstract CTX-M-3 is the most common extended-spectrum β-lactamase produced by Enterobacteriaceae in Taiwan. The present study was conducted to characterise the genetic environment surrounding blaCTX-M-3 . A total of 11 ceftriaxone-resistant isolates were studied: Escherichia coli ( n = 4), Klebsiella pneumoniae ( n = 5) and Salmonella enterica serotypes Anatum (SA831R) and Potsdam (SC72). Molecular methods used included polymerase chain reaction, sequencing, DNA–DNA hybridisation, conjugation, physical mapping and restriction fragment length polymorphism (RFLP) analysis. All isolates examined carried blaCTX-M-3 on large plasmids (>70 kb). The resistance plasmids of the two Salmonella and two K. pneumoniae strains (KP104 and KP116) were confirmed to be conjugative in vitro. RFLP analysis indicated that the plasmids were different. Physical mapping also revealed the difference between the two Salmonella plasmids, pSA831R (82 kb) and pSC72 (74 kb). An insertion sequence, IS Ecp1 , was found upstream of each blaCTX-M-3 gene. However, sequencing of downstream regions of the bla genes showed two different patterns: the presence of orf477 in pSA831R and of orf1 - mucA in pSC72, pKP104 and pKP116. IncI1-type oriT and nikA sequences were present in the plasmids of all the clinical isolates tested, except S. Anatum. Different blaCTX-M-3 -carrying plasmids were identified among the enterobacteria studied. The presence of IS Ecp1 in all isolates may be associated with the widespread resistance among Enterobacteriaceae. Although the plasmids were not identical, they appeared to belong to the same incompatibility group (IncI1-like plasmids), suggesting that they are genetically related but may have evolved divergently over time.</description><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antimicrobial resistance</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Ceftriaxone - pharmacology</subject><subject>Conjugation</subject><subject>Conjugation, Genetic</subject><subject>DNA Transposable Elements</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Enterobacteriaceae</subject><subject>Enterobacteriaceae - drug effects</subject><subject>Enterobacteriaceae - genetics</subject><subject>Enterobacteriaceae - isolation & purification</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Escherichia coli</subject><subject>Hospitals, University</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Klebsiella pneumoniae</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasmids - genetics</subject><subject>Restriction Mapping</subject><subject>Salmonella enterica</subject><subject>Taiwan</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2O1DAQRiMEYpqBKyCzYHZp7MSx4w0SioYfaRALGomdVbErM24Su7GTgb4FZ-EgnAm3uqVBbGDlzfu-KvlVUTxjdM0oEy-2a7cFP7vJGbheV5SKNWNryup7xYq1siqlYvX9YkVVxcu2keqseJTSllLW1Lx5WJwxWcmmEs2q-NHdQAQzY3QJZhc8CQPZjZAmZxNBb4J1_pp0m8_l-7Im-H1Gb9GWaYdmjstEfv0sx5yHCRKSIYaJXPrcFvpjKRgEJC6FEWa0BGYCZPHuFmNy857chLRzM4zEebIB9w384-LBAGPCJ6f3vPj0-nLTvS2vPrx51726Kg1XbC4NCG4ZmJbCwA2ThqOooGkFygElbUH1ikIDqHpDe8S-5Y21wjIGIAXl9XlxcezdxfB1wTTrySWD4wgew5K0pBVvaC3_CVa0VoxzkUF1BE0MKUUc9C66CeJeM6oP3vRW_-FNH7xpxnT2lrNPT0OWfkJ7lzyJysDzEwDJwDhE8MalO64VTDKqMtcdOcx_d-sw6mRc1ojWxWxM2-D-a52Xf7WY0fmMjV9wj2kbluizHM10qjTVHw-HdrgzKijlrVD1byTM1V0</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Liu, S.Y</creator><creator>Su, L.H</creator><creator>Yeh, Y.L</creator><creator>Chu, C</creator><creator>Lai, J.C</creator><creator>Chiu, C.H</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Characterisation of plasmids encoding CTX-M-3 extended-spectrum β-lactamase from Enterobacteriaceae isolated at a university hospital in Taiwan</title><author>Liu, S.Y ; Su, L.H ; Yeh, Y.L ; Chu, C ; Lai, J.C ; Chiu, C.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-ca64d1ac80af4c17c4e62a586e7fe708a9b90a5ae9bc0beeb845dd6d11aa76043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antimicrobial resistance</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Ceftriaxone - pharmacology</topic><topic>Conjugation</topic><topic>Conjugation, Genetic</topic><topic>DNA Transposable Elements</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Enterobacteriaceae</topic><topic>Enterobacteriaceae - drug effects</topic><topic>Enterobacteriaceae - genetics</topic><topic>Enterobacteriaceae - isolation & purification</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Escherichia coli</topic><topic>Hospitals, University</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Klebsiella pneumoniae</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasmids - genetics</topic><topic>Restriction Mapping</topic><topic>Salmonella enterica</topic><topic>Taiwan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, S.Y</creatorcontrib><creatorcontrib>Su, L.H</creatorcontrib><creatorcontrib>Yeh, Y.L</creatorcontrib><creatorcontrib>Chu, C</creatorcontrib><creatorcontrib>Lai, J.C</creatorcontrib><creatorcontrib>Chiu, C.H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, S.Y</au><au>Su, L.H</au><au>Yeh, Y.L</au><au>Chu, C</au><au>Lai, J.C</au><au>Chiu, C.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of plasmids encoding CTX-M-3 extended-spectrum β-lactamase from Enterobacteriaceae isolated at a university hospital in Taiwan</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>29</volume><issue>4</issue><spage>440</spage><epage>445</epage><pages>440-445</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>Abstract CTX-M-3 is the most common extended-spectrum β-lactamase produced by Enterobacteriaceae in Taiwan. The present study was conducted to characterise the genetic environment surrounding blaCTX-M-3 . A total of 11 ceftriaxone-resistant isolates were studied: Escherichia coli ( n = 4), Klebsiella pneumoniae ( n = 5) and Salmonella enterica serotypes Anatum (SA831R) and Potsdam (SC72). Molecular methods used included polymerase chain reaction, sequencing, DNA–DNA hybridisation, conjugation, physical mapping and restriction fragment length polymorphism (RFLP) analysis. All isolates examined carried blaCTX-M-3 on large plasmids (>70 kb). The resistance plasmids of the two Salmonella and two K. pneumoniae strains (KP104 and KP116) were confirmed to be conjugative in vitro. RFLP analysis indicated that the plasmids were different. Physical mapping also revealed the difference between the two Salmonella plasmids, pSA831R (82 kb) and pSC72 (74 kb). An insertion sequence, IS Ecp1 , was found upstream of each blaCTX-M-3 gene. However, sequencing of downstream regions of the bla genes showed two different patterns: the presence of orf477 in pSA831R and of orf1 - mucA in pSC72, pKP104 and pKP116. IncI1-type oriT and nikA sequences were present in the plasmids of all the clinical isolates tested, except S. Anatum. Different blaCTX-M-3 -carrying plasmids were identified among the enterobacteria studied. The presence of IS Ecp1 in all isolates may be associated with the widespread resistance among Enterobacteriaceae. Although the plasmids were not identical, they appeared to belong to the same incompatibility group (IncI1-like plasmids), suggesting that they are genetically related but may have evolved divergently over time.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>17275265</pmid><doi>10.1016/j.ijantimicag.2006.11.013</doi><tpages>6</tpages></addata></record> |
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| subjects | Antibiotics. Antiinfectious agents. Antiparasitic agents Antimicrobial resistance beta-Lactamases - genetics beta-Lactamases - metabolism Biological and medical sciences Ceftriaxone - pharmacology Conjugation Conjugation, Genetic DNA Transposable Elements Drug Resistance, Bacterial - genetics Enterobacteriaceae Enterobacteriaceae - drug effects Enterobacteriaceae - genetics Enterobacteriaceae - isolation & purification Enterobacteriaceae Infections - microbiology Escherichia coli Hospitals, University Humans Infectious Disease Klebsiella pneumoniae Medical sciences Pharmacology. Drug treatments Plasmids - genetics Restriction Mapping Salmonella enterica Taiwan |
| title | Characterisation of plasmids encoding CTX-M-3 extended-spectrum β-lactamase from Enterobacteriaceae isolated at a university hospital in Taiwan |
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