Irsogladine maleate counters the interleukin-1β-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1β-induced zonula occludens protein-1 levels in human gingival epithelial cells
Background and Objective: Irsogladine maleate counters gap junctional intercellular communication reduction induced by interleukin‐8 or Actinobacillus actinomycetemcomitans in cultured human gingival epithelial cells. Interleukin‐1β is involved in periodontal disease. Little is known, however, abou...
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| creator | Fujita, T. Ashikaga, A. Shiba, H. Kajiya, M. Kishimoto, A. Hirata, R. Tsunekuni, N. Hirono, C. Kawaguchi, H. Shiba, Y. Kurihara, H. |
| description | Background and Objective: Irsogladine maleate counters gap junctional intercellular communication reduction induced by interleukin‐8 or Actinobacillus actinomycetemcomitans in cultured human gingival epithelial cells. Interleukin‐1β is involved in periodontal disease. Little is known, however, about the effect of interleukin‐1β on intercellular junctional complexes in human gingival epithelial cells. Furthermore, irsogladine maleate may affect the actions of interleukin‐1β. In this study, we examined how interleukin‐1β affected gap junctional intercellular communication, connexin 43 and zonula occludens protein‐1, and how irsogladine maleate modulated the interleukin‐1β‐induced changes in the intercellular junctional complexes in human gingival epithelial cells.
Material and Methods: Human gingival epithelial cells were exposed to interleukin‐1β, with or without irsogladine maleate. Connexin 43 and zonula occludens protein‐1 were examined at mRNA and protein levels by real‐time polymerase chain reaction and western blotting, respectively. Gap junctional intercellular communication was determined using the dye transfer method. The expression of zonula occludens protein‐1 was also confirmed by immunofluorescence.
Results: Interleukin‐1β decreased connexin 43 mRNA levels, but increased zonula occludens protein‐1 mRNA levels. Irsogladine maleate countered the interleukin‐1β‐induced reduction in gap junctional intercellular communication and connexin 43 levels. However, irsogladine maleate did not influence the increased zonula occludens protein‐1 levels.
Conclusion: The effect of interleukin‐1β on gap junctional intercellular communication and tight junctions of human gingival epithelial cells is different. The recovery of gap junctional intercellular communication by irsogladine maleate in the gingival epithelium may be a normal process in gingival epithelial homeostasis. |
| doi_str_mv | 10.1111/j.1600-0765.2007.01000.x |
| format | Article |
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Material and Methods: Human gingival epithelial cells were exposed to interleukin‐1β, with or without irsogladine maleate. Connexin 43 and zonula occludens protein‐1 were examined at mRNA and protein levels by real‐time polymerase chain reaction and western blotting, respectively. Gap junctional intercellular communication was determined using the dye transfer method. The expression of zonula occludens protein‐1 was also confirmed by immunofluorescence.
Results: Interleukin‐1β decreased connexin 43 mRNA levels, but increased zonula occludens protein‐1 mRNA levels. Irsogladine maleate countered the interleukin‐1β‐induced reduction in gap junctional intercellular communication and connexin 43 levels. However, irsogladine maleate did not influence the increased zonula occludens protein‐1 levels.
Conclusion: The effect of interleukin‐1β on gap junctional intercellular communication and tight junctions of human gingival epithelial cells is different. The recovery of gap junctional intercellular communication by irsogladine maleate in the gingival epithelium may be a normal process in gingival epithelial homeostasis.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2007.01000.x</identifier><identifier>PMID: 18230110</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antineoplastic Agents - pharmacology ; Biological and medical sciences ; Connexin 43 - metabolism ; Dentistry ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Gap Junctions - drug effects ; Gap Junctions - metabolism ; gap-junctional intercellular communication ; Gingiva - cytology ; human gingival epithelial cells ; Humans ; Interleukin-1beta - antagonists & inhibitors ; Interleukin-1beta - metabolism ; Interleukin-1beta - pharmacology ; interleukin-1β ; irsogladine maleate ; Medical sciences ; Membrane Proteins - metabolism ; Otorhinolaryngology. Stomatology ; Phosphoproteins - metabolism ; RNA, Messenger - metabolism ; Triazines - pharmacology ; Zonula Occludens-1 Protein</subject><ispartof>Journal of periodontal research, 2008-02, Vol.43 (1), p.96-102</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4350-8addb83a6c618620053c3bfcf91e000001caf37b18511e2340f4ccb43ed3ebcb3</citedby><cites>FETCH-LOGICAL-c4350-8addb83a6c618620053c3bfcf91e000001caf37b18511e2340f4ccb43ed3ebcb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0765.2007.01000.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0765.2007.01000.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20016935$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18230110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujita, T.</creatorcontrib><creatorcontrib>Ashikaga, A.</creatorcontrib><creatorcontrib>Shiba, H.</creatorcontrib><creatorcontrib>Kajiya, M.</creatorcontrib><creatorcontrib>Kishimoto, A.</creatorcontrib><creatorcontrib>Hirata, R.</creatorcontrib><creatorcontrib>Tsunekuni, N.</creatorcontrib><creatorcontrib>Hirono, C.</creatorcontrib><creatorcontrib>Kawaguchi, H.</creatorcontrib><creatorcontrib>Shiba, Y.</creatorcontrib><creatorcontrib>Kurihara, H.</creatorcontrib><title>Irsogladine maleate counters the interleukin-1β-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1β-induced zonula occludens protein-1 levels in human gingival epithelial cells</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objective: Irsogladine maleate counters gap junctional intercellular communication reduction induced by interleukin‐8 or Actinobacillus actinomycetemcomitans in cultured human gingival epithelial cells. Interleukin‐1β is involved in periodontal disease. Little is known, however, about the effect of interleukin‐1β on intercellular junctional complexes in human gingival epithelial cells. Furthermore, irsogladine maleate may affect the actions of interleukin‐1β. In this study, we examined how interleukin‐1β affected gap junctional intercellular communication, connexin 43 and zonula occludens protein‐1, and how irsogladine maleate modulated the interleukin‐1β‐induced changes in the intercellular junctional complexes in human gingival epithelial cells.
Material and Methods: Human gingival epithelial cells were exposed to interleukin‐1β, with or without irsogladine maleate. Connexin 43 and zonula occludens protein‐1 were examined at mRNA and protein levels by real‐time polymerase chain reaction and western blotting, respectively. Gap junctional intercellular communication was determined using the dye transfer method. The expression of zonula occludens protein‐1 was also confirmed by immunofluorescence.
Results: Interleukin‐1β decreased connexin 43 mRNA levels, but increased zonula occludens protein‐1 mRNA levels. Irsogladine maleate countered the interleukin‐1β‐induced reduction in gap junctional intercellular communication and connexin 43 levels. However, irsogladine maleate did not influence the increased zonula occludens protein‐1 levels.
Conclusion: The effect of interleukin‐1β on gap junctional intercellular communication and tight junctions of human gingival epithelial cells is different. The recovery of gap junctional intercellular communication by irsogladine maleate in the gingival epithelium may be a normal process in gingival epithelial homeostasis.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Connexin 43 - metabolism</subject><subject>Dentistry</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Gap Junctions - drug effects</subject><subject>Gap Junctions - metabolism</subject><subject>gap-junctional intercellular communication</subject><subject>Gingiva - cytology</subject><subject>human gingival epithelial cells</subject><subject>Humans</subject><subject>Interleukin-1beta - antagonists & inhibitors</subject><subject>Interleukin-1beta - metabolism</subject><subject>Interleukin-1beta - pharmacology</subject><subject>interleukin-1β</subject><subject>irsogladine maleate</subject><subject>Medical sciences</subject><subject>Membrane Proteins - metabolism</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Phosphoproteins - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Triazines - pharmacology</subject><subject>Zonula Occludens-1 Protein</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2O0zAUhS0EYsrAKyBvYJdix_nrggWM5g9VICEQ3VmOc9Nxx3GCf4YOj8WD8Bq8Bjapyg7hTa7l75x7lIMQpmRJ43m1W9KKkIzUVbnMCamXhBJClvsHaHF8eIgWhOR5xoqmOEFPnNtFJK_q1WN0QpucEUrJAv26tm7catEpA3gQGoQHLMdgPFiH_Q1glUYN4VaZjP78kSnTBQkddmGaLDinRhMZvBVTtgtG-ngXelZJ0DpoYaPhMASjpEivuA0edyM4bEaPRd-D9P_c9H000QWPUurQgXF4sqOHBGENd6Bd2n8TBhFTKLNVd3E_TCpaahXHlMI9RY96oR08O3xP0eeL809nV9n6w-X12Zt1JgtWkqwRXdc2TFSyok0Vf23JJGt72a8okHSoFD2rW9qUlELOCtIXUrYFg45BK1t2il7OvjHj1wDO80G5lEAYGIPjNcmLoqlZBJsZlHZ0zkLPJ6sGYe85JTyVzHc8dclTlzyVzP-UzPdR-vywI7QDdH-Fh1Yj8OIACCeF7q0wUrkjF81otWJl5F7P3Del4f6_A_B3H8_TFPXZrFfOw_6oF_aWVzWrS_7l_SXfbMq3m4t1xa_Yb2zC2oc</recordid><startdate>200802</startdate><enddate>200802</enddate><creator>Fujita, T.</creator><creator>Ashikaga, A.</creator><creator>Shiba, H.</creator><creator>Kajiya, M.</creator><creator>Kishimoto, A.</creator><creator>Hirata, R.</creator><creator>Tsunekuni, N.</creator><creator>Hirono, C.</creator><creator>Kawaguchi, H.</creator><creator>Shiba, Y.</creator><creator>Kurihara, H.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200802</creationdate><title>Irsogladine maleate counters the interleukin-1β-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1β-induced zonula occludens protein-1 levels in human gingival epithelial cells</title><author>Fujita, T. ; Ashikaga, A. ; Shiba, H. ; Kajiya, M. ; Kishimoto, A. ; Hirata, R. ; Tsunekuni, N. ; Hirono, C. ; Kawaguchi, H. ; Shiba, Y. ; Kurihara, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4350-8addb83a6c618620053c3bfcf91e000001caf37b18511e2340f4ccb43ed3ebcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Connexin 43 - metabolism</topic><topic>Dentistry</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Gap Junctions - drug effects</topic><topic>Gap Junctions - metabolism</topic><topic>gap-junctional intercellular communication</topic><topic>Gingiva - cytology</topic><topic>human gingival epithelial cells</topic><topic>Humans</topic><topic>Interleukin-1beta - antagonists & inhibitors</topic><topic>Interleukin-1beta - metabolism</topic><topic>Interleukin-1beta - pharmacology</topic><topic>interleukin-1β</topic><topic>irsogladine maleate</topic><topic>Medical sciences</topic><topic>Membrane Proteins - metabolism</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Phosphoproteins - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Triazines - pharmacology</topic><topic>Zonula Occludens-1 Protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujita, T.</creatorcontrib><creatorcontrib>Ashikaga, A.</creatorcontrib><creatorcontrib>Shiba, H.</creatorcontrib><creatorcontrib>Kajiya, M.</creatorcontrib><creatorcontrib>Kishimoto, A.</creatorcontrib><creatorcontrib>Hirata, R.</creatorcontrib><creatorcontrib>Tsunekuni, N.</creatorcontrib><creatorcontrib>Hirono, C.</creatorcontrib><creatorcontrib>Kawaguchi, H.</creatorcontrib><creatorcontrib>Shiba, Y.</creatorcontrib><creatorcontrib>Kurihara, H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujita, T.</au><au>Ashikaga, A.</au><au>Shiba, H.</au><au>Kajiya, M.</au><au>Kishimoto, A.</au><au>Hirata, R.</au><au>Tsunekuni, N.</au><au>Hirono, C.</au><au>Kawaguchi, H.</au><au>Shiba, Y.</au><au>Kurihara, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Irsogladine maleate counters the interleukin-1β-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1β-induced zonula occludens protein-1 levels in human gingival epithelial cells</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2008-02</date><risdate>2008</risdate><volume>43</volume><issue>1</issue><spage>96</spage><epage>102</epage><pages>96-102</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective: Irsogladine maleate counters gap junctional intercellular communication reduction induced by interleukin‐8 or Actinobacillus actinomycetemcomitans in cultured human gingival epithelial cells. Interleukin‐1β is involved in periodontal disease. Little is known, however, about the effect of interleukin‐1β on intercellular junctional complexes in human gingival epithelial cells. Furthermore, irsogladine maleate may affect the actions of interleukin‐1β. In this study, we examined how interleukin‐1β affected gap junctional intercellular communication, connexin 43 and zonula occludens protein‐1, and how irsogladine maleate modulated the interleukin‐1β‐induced changes in the intercellular junctional complexes in human gingival epithelial cells.
Material and Methods: Human gingival epithelial cells were exposed to interleukin‐1β, with or without irsogladine maleate. Connexin 43 and zonula occludens protein‐1 were examined at mRNA and protein levels by real‐time polymerase chain reaction and western blotting, respectively. Gap junctional intercellular communication was determined using the dye transfer method. The expression of zonula occludens protein‐1 was also confirmed by immunofluorescence.
Results: Interleukin‐1β decreased connexin 43 mRNA levels, but increased zonula occludens protein‐1 mRNA levels. Irsogladine maleate countered the interleukin‐1β‐induced reduction in gap junctional intercellular communication and connexin 43 levels. However, irsogladine maleate did not influence the increased zonula occludens protein‐1 levels.
Conclusion: The effect of interleukin‐1β on gap junctional intercellular communication and tight junctions of human gingival epithelial cells is different. The recovery of gap junctional intercellular communication by irsogladine maleate in the gingival epithelium may be a normal process in gingival epithelial homeostasis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18230110</pmid><doi>10.1111/j.1600-0765.2007.01000.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Antineoplastic Agents - pharmacology Biological and medical sciences Connexin 43 - metabolism Dentistry Epithelial Cells - cytology Epithelial Cells - drug effects Gap Junctions - drug effects Gap Junctions - metabolism gap-junctional intercellular communication Gingiva - cytology human gingival epithelial cells Humans Interleukin-1beta - antagonists & inhibitors Interleukin-1beta - metabolism Interleukin-1beta - pharmacology interleukin-1β irsogladine maleate Medical sciences Membrane Proteins - metabolism Otorhinolaryngology. Stomatology Phosphoproteins - metabolism RNA, Messenger - metabolism Triazines - pharmacology Zonula Occludens-1 Protein |
| title | Irsogladine maleate counters the interleukin-1β-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1β-induced zonula occludens protein-1 levels in human gingival epithelial cells |
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