Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters

We have generated a recombinant CHO cell line expressing the fusion protein EpoFc. After selection and screening, protein expression, gene and mRNA copy numbers were analysed in order to gain more information on the influence of genetic parameters on the productivity and stability of production cell...

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Veröffentlicht in:	Journal of biotechnology 2007-03, Vol.128 (4), p.716-725
Hauptverfasser:	Lattenmayer, Christine, Trummer, Evelyn, Schriebl, Kornelia, Vorauer-Uhl, Karola, Mueller, Dethardt, Katinger, Hermann, Kunert, Renate
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Blotting, Northern Blotting, Southern Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics CHO Cells Cricetinae Cricetulus Erythropoietin - biosynthesis Erythropoietin - genetics Fundamental and applied biological sciences. Psychology Gene copy number Gene Dosage Genetic characterisation Genetic parameters Membrane Proteins - biosynthesis Membrane Proteins - genetics Polymerase Chain Reaction Quantitative PCR Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics RNA, Messenger - analysis RNA, Messenger - genetics Taqman
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