Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation
Cell interactions with the extracellular matrix (ECM) play a critical role in their physiology. Here, we sought to determine the role of exogenous and endogenous ECM in the differentiation of nonhuman primate ESCs. We evaluated cell differentiation from expression of lineage gene mRNA and proteins u...
Gespeichert in:
Veröffentlicht in: | Stem cells (Dayton, Ohio) Ohio), 2007-03, Vol.25 (3), p.553-561 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 561 |
---|---|
container_issue | 3 |
container_start_page | 553 |
container_title | Stem cells (Dayton, Ohio) |
container_volume | 25 |
creator | Chen, Silvia S Fitzgerald, Wendy Zimmerberg, Joshua Kleinman, Hynda K Margolis, Leonid |
description | Cell interactions with the extracellular matrix (ECM) play a critical role in their physiology. Here, we sought to determine the role of exogenous and endogenous ECM in the differentiation of nonhuman primate ESCs. We evaluated cell differentiation from expression of lineage gene mRNA and proteins using real-time polymerase chain reaction and immunohistochemistry. We found that ESCs that attached to and spread upon highly adhesive collagen do not differentiate efficiently, whereas on the less adhesive Matrigel, ESCs form aggregates and differentiate along mesoderm and especially endoderm lineages. To further decrease ESC attachment to the substrate, we cultured them either on nonadhesive agarose or in suspension. In both cases, ESCs formed aggregates and efficiently differentiated along endoderm and mesoderm lineages, most strikingly into cardiomyocytes. Aggregates formed by thus-differentiated ESCs started to beat with a frequency of 50-100 beats per minute and continued to beat for approximately a month. In spite of the presence of exogenous ECM, ESCs were dependent on endogenous ECM for their survival and differentiation, as the inhibition of endogenous collagen induced a gradual loss of ESCs and neither a simple matrix, such as type I collagen, nor the complex matrix Matrigel was able to rescue these cells. In conclusion, adhesiveness to various ECM and nonbiological substrates determines the differentiation of ESCs in such a way that efficient cell-cell aggregation, together with less efficient cell attachment and spreading, results in more efficient cell differentiation. |
doi_str_mv | 10.1634/stemcells.2006-0419 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70234876</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70234876</sourcerecordid><originalsourceid>FETCH-LOGICAL-c495t-61ef18dfb15a2220ce28738b28b00e7c44099f286baadea77294c32bbb3ed87b3</originalsourceid><addsrcrecordid>eNqFUctKA0EQHEQxMfoFgszJ28R57TyOEnxBwIuel5ndXlnZR5yZQPL37pigR09ddFcV3V0IXTO6ZErIu5igr6Dr4pJTqgiVzJ6gOSukJdIyczphqhQpqLUzdBHjJ6VMFsacoxnTQvCCyTlyq8mBZBvshhpnQGCXgsto27mAe5dCu8PtkGDqpnYcIg7wMc0SYOh92I9DW-G8zY8c123TQIAhtS6zL9FZ47oIV8e6QO-PD2-rZ7J-fXpZ3a9JJW2RiGLQMFM3nhWOc04r4EYL47nxlIKupJzuaLhR3rkanNbcykpw772A2mgvFuj24LsJ49cWYir7NuaF3ADjNpaaciGNVv8SmdXaKsEmojgQqzDGGKApN6HtXdiXjJY5gvI3gjJHUOYIJtXN0X7re6j_NMefi2_aHIcL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19779631</pqid></control><display><type>article</type><title>Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Chen, Silvia S ; Fitzgerald, Wendy ; Zimmerberg, Joshua ; Kleinman, Hynda K ; Margolis, Leonid</creator><creatorcontrib>Chen, Silvia S ; Fitzgerald, Wendy ; Zimmerberg, Joshua ; Kleinman, Hynda K ; Margolis, Leonid</creatorcontrib><description>Cell interactions with the extracellular matrix (ECM) play a critical role in their physiology. Here, we sought to determine the role of exogenous and endogenous ECM in the differentiation of nonhuman primate ESCs. We evaluated cell differentiation from expression of lineage gene mRNA and proteins using real-time polymerase chain reaction and immunohistochemistry. We found that ESCs that attached to and spread upon highly adhesive collagen do not differentiate efficiently, whereas on the less adhesive Matrigel, ESCs form aggregates and differentiate along mesoderm and especially endoderm lineages. To further decrease ESC attachment to the substrate, we cultured them either on nonadhesive agarose or in suspension. In both cases, ESCs formed aggregates and efficiently differentiated along endoderm and mesoderm lineages, most strikingly into cardiomyocytes. Aggregates formed by thus-differentiated ESCs started to beat with a frequency of 50-100 beats per minute and continued to beat for approximately a month. In spite of the presence of exogenous ECM, ESCs were dependent on endogenous ECM for their survival and differentiation, as the inhibition of endogenous collagen induced a gradual loss of ESCs and neither a simple matrix, such as type I collagen, nor the complex matrix Matrigel was able to rescue these cells. In conclusion, adhesiveness to various ECM and nonbiological substrates determines the differentiation of ESCs in such a way that efficient cell-cell aggregation, together with less efficient cell attachment and spreading, results in more efficient cell differentiation.</description><identifier>ISSN: 1066-5099</identifier><identifier>EISSN: 1549-4918</identifier><identifier>DOI: 10.1634/stemcells.2006-0419</identifier><identifier>PMID: 17332514</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cell Communication ; Cell Culture Techniques ; Cell Differentiation ; Cell Division ; Collagen ; Drug Combinations ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - physiology ; Extracellular Matrix - physiology ; Gene Expression Regulation ; Immunohistochemistry ; Laminin ; Mice ; Primates ; Proteoglycans ; Reverse Transcriptase Polymerase Chain Reaction</subject><ispartof>Stem cells (Dayton, Ohio), 2007-03, Vol.25 (3), p.553-561</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-61ef18dfb15a2220ce28738b28b00e7c44099f286baadea77294c32bbb3ed87b3</citedby><cites>FETCH-LOGICAL-c495t-61ef18dfb15a2220ce28738b28b00e7c44099f286baadea77294c32bbb3ed87b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17332514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Silvia S</creatorcontrib><creatorcontrib>Fitzgerald, Wendy</creatorcontrib><creatorcontrib>Zimmerberg, Joshua</creatorcontrib><creatorcontrib>Kleinman, Hynda K</creatorcontrib><creatorcontrib>Margolis, Leonid</creatorcontrib><title>Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation</title><title>Stem cells (Dayton, Ohio)</title><addtitle>Stem Cells</addtitle><description>Cell interactions with the extracellular matrix (ECM) play a critical role in their physiology. Here, we sought to determine the role of exogenous and endogenous ECM in the differentiation of nonhuman primate ESCs. We evaluated cell differentiation from expression of lineage gene mRNA and proteins using real-time polymerase chain reaction and immunohistochemistry. We found that ESCs that attached to and spread upon highly adhesive collagen do not differentiate efficiently, whereas on the less adhesive Matrigel, ESCs form aggregates and differentiate along mesoderm and especially endoderm lineages. To further decrease ESC attachment to the substrate, we cultured them either on nonadhesive agarose or in suspension. In both cases, ESCs formed aggregates and efficiently differentiated along endoderm and mesoderm lineages, most strikingly into cardiomyocytes. Aggregates formed by thus-differentiated ESCs started to beat with a frequency of 50-100 beats per minute and continued to beat for approximately a month. In spite of the presence of exogenous ECM, ESCs were dependent on endogenous ECM for their survival and differentiation, as the inhibition of endogenous collagen induced a gradual loss of ESCs and neither a simple matrix, such as type I collagen, nor the complex matrix Matrigel was able to rescue these cells. In conclusion, adhesiveness to various ECM and nonbiological substrates determines the differentiation of ESCs in such a way that efficient cell-cell aggregation, together with less efficient cell attachment and spreading, results in more efficient cell differentiation.</description><subject>Animals</subject><subject>Cell Communication</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Collagen</subject><subject>Drug Combinations</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Extracellular Matrix - physiology</subject><subject>Gene Expression Regulation</subject><subject>Immunohistochemistry</subject><subject>Laminin</subject><subject>Mice</subject><subject>Primates</subject><subject>Proteoglycans</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><issn>1066-5099</issn><issn>1549-4918</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctKA0EQHEQxMfoFgszJ28R57TyOEnxBwIuel5ndXlnZR5yZQPL37pigR09ddFcV3V0IXTO6ZErIu5igr6Dr4pJTqgiVzJ6gOSukJdIyczphqhQpqLUzdBHjJ6VMFsacoxnTQvCCyTlyq8mBZBvshhpnQGCXgsto27mAe5dCu8PtkGDqpnYcIg7wMc0SYOh92I9DW-G8zY8c123TQIAhtS6zL9FZ47oIV8e6QO-PD2-rZ7J-fXpZ3a9JJW2RiGLQMFM3nhWOc04r4EYL47nxlIKupJzuaLhR3rkanNbcykpw772A2mgvFuj24LsJ49cWYir7NuaF3ADjNpaaciGNVv8SmdXaKsEmojgQqzDGGKApN6HtXdiXjJY5gvI3gjJHUOYIJtXN0X7re6j_NMefi2_aHIcL</recordid><startdate>200703</startdate><enddate>200703</enddate><creator>Chen, Silvia S</creator><creator>Fitzgerald, Wendy</creator><creator>Zimmerberg, Joshua</creator><creator>Kleinman, Hynda K</creator><creator>Margolis, Leonid</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200703</creationdate><title>Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation</title><author>Chen, Silvia S ; Fitzgerald, Wendy ; Zimmerberg, Joshua ; Kleinman, Hynda K ; Margolis, Leonid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-61ef18dfb15a2220ce28738b28b00e7c44099f286baadea77294c32bbb3ed87b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Cell Communication</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Collagen</topic><topic>Drug Combinations</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Extracellular Matrix - physiology</topic><topic>Gene Expression Regulation</topic><topic>Immunohistochemistry</topic><topic>Laminin</topic><topic>Mice</topic><topic>Primates</topic><topic>Proteoglycans</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Silvia S</creatorcontrib><creatorcontrib>Fitzgerald, Wendy</creatorcontrib><creatorcontrib>Zimmerberg, Joshua</creatorcontrib><creatorcontrib>Kleinman, Hynda K</creatorcontrib><creatorcontrib>Margolis, Leonid</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cells (Dayton, Ohio)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Silvia S</au><au>Fitzgerald, Wendy</au><au>Zimmerberg, Joshua</au><au>Kleinman, Hynda K</au><au>Margolis, Leonid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation</atitle><jtitle>Stem cells (Dayton, Ohio)</jtitle><addtitle>Stem Cells</addtitle><date>2007-03</date><risdate>2007</risdate><volume>25</volume><issue>3</issue><spage>553</spage><epage>561</epage><pages>553-561</pages><issn>1066-5099</issn><eissn>1549-4918</eissn><abstract>Cell interactions with the extracellular matrix (ECM) play a critical role in their physiology. Here, we sought to determine the role of exogenous and endogenous ECM in the differentiation of nonhuman primate ESCs. We evaluated cell differentiation from expression of lineage gene mRNA and proteins using real-time polymerase chain reaction and immunohistochemistry. We found that ESCs that attached to and spread upon highly adhesive collagen do not differentiate efficiently, whereas on the less adhesive Matrigel, ESCs form aggregates and differentiate along mesoderm and especially endoderm lineages. To further decrease ESC attachment to the substrate, we cultured them either on nonadhesive agarose or in suspension. In both cases, ESCs formed aggregates and efficiently differentiated along endoderm and mesoderm lineages, most strikingly into cardiomyocytes. Aggregates formed by thus-differentiated ESCs started to beat with a frequency of 50-100 beats per minute and continued to beat for approximately a month. In spite of the presence of exogenous ECM, ESCs were dependent on endogenous ECM for their survival and differentiation, as the inhibition of endogenous collagen induced a gradual loss of ESCs and neither a simple matrix, such as type I collagen, nor the complex matrix Matrigel was able to rescue these cells. In conclusion, adhesiveness to various ECM and nonbiological substrates determines the differentiation of ESCs in such a way that efficient cell-cell aggregation, together with less efficient cell attachment and spreading, results in more efficient cell differentiation.</abstract><cop>United States</cop><pmid>17332514</pmid><doi>10.1634/stemcells.2006-0419</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1066-5099 |
ispartof | Stem cells (Dayton, Ohio), 2007-03, Vol.25 (3), p.553-561 |
issn | 1066-5099 1549-4918 |
language | eng |
recordid | cdi_proquest_miscellaneous_70234876 |
source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | Animals Cell Communication Cell Culture Techniques Cell Differentiation Cell Division Collagen Drug Combinations Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology Extracellular Matrix - physiology Gene Expression Regulation Immunohistochemistry Laminin Mice Primates Proteoglycans Reverse Transcriptase Polymerase Chain Reaction |
title | Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T09%3A26%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cell-cell%20and%20cell-extracellular%20matrix%20interactions%20regulate%20embryonic%20stem%20cell%20differentiation&rft.jtitle=Stem%20cells%20(Dayton,%20Ohio)&rft.au=Chen,%20Silvia%20S&rft.date=2007-03&rft.volume=25&rft.issue=3&rft.spage=553&rft.epage=561&rft.pages=553-561&rft.issn=1066-5099&rft.eissn=1549-4918&rft_id=info:doi/10.1634/stemcells.2006-0419&rft_dat=%3Cproquest_cross%3E70234876%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19779631&rft_id=info:pmid/17332514&rfr_iscdi=true |