Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method
A sensitive high performance liquid chromatography tandem mass spectrometry (LC–MS/MS) method has been developed for simultaneous determination of procaine and its metabolite p-aminobenzoic acid (PABA). N-Acetylprocainamide (NAPA) was used as an internal standard for procaine and PABA analysis. This...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-03, Vol.847 (2), p.224-230 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Dhananjeyan, Mugunthu R. Bykowski, Crystal Trendel, Jill A. Sarver, Jeffrey G. Ando, Howard Erhardt, Paul W. |
| description | A sensitive high performance liquid chromatography tandem mass spectrometry (LC–MS/MS) method has been developed for simultaneous determination of procaine and its metabolite
p-aminobenzoic acid (PABA).
N-Acetylprocainamide (NAPA) was used as an internal standard for procaine and PABA analysis. This assay method has also been validated in terms of linearity, lower limit of detection, lower limit of quantitation, accuracy and precision as per ICH guidelines. Chromatography was carried out on an XTerra™ MS C
18 column and mass spectrometric analysis was performed using a Quattro Micro™ mass spectrometer working with electro-spray ionization (ESI) source in the positive ion mode. Enhanced selectivity was achieved using multiple reaction monitoring (MRM) functions,
m/
z 237
→
100,
m/
z 138
→
120, and
m/
z 278
→
205 for procaine, PABA and NAPA, respectively. Retention times for PABA, procaine and NAPA were 4.0, 4.7 and 5.8
min, respectively. Linearity for each calibration curve was observed across a range from 100
nM to 5000
nM for PABA, and from 10
nM to 5000
nM for procaine. The intra- and inter-day relative standard deviations (RSD) were |
| doi_str_mv | 10.1016/j.jchromb.2006.10.004 |
| format | Article |
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p-aminobenzoic acid (PABA).
N-Acetylprocainamide (NAPA) was used as an internal standard for procaine and PABA analysis. This assay method has also been validated in terms of linearity, lower limit of detection, lower limit of quantitation, accuracy and precision as per ICH guidelines. Chromatography was carried out on an XTerra™ MS C
18 column and mass spectrometric analysis was performed using a Quattro Micro™ mass spectrometer working with electro-spray ionization (ESI) source in the positive ion mode. Enhanced selectivity was achieved using multiple reaction monitoring (MRM) functions,
m/
z 237
→
100,
m/
z 138
→
120, and
m/
z 278
→
205 for procaine, PABA and NAPA, respectively. Retention times for PABA, procaine and NAPA were 4.0, 4.7 and 5.8
min, respectively. Linearity for each calibration curve was observed across a range from 100
nM to 5000
nM for PABA, and from 10
nM to 5000
nM for procaine. The intra- and inter-day relative standard deviations (RSD) were <5%.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2006.10.004</identifier><identifier>PMID: 17070118</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>4-Aminobenzoic Acid - analysis ; 4-Aminobenzoic Acid - chemistry ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Diseases of the skin. Cosmetics ; Esters ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; LC–MS/MS ; Local anesthetics ; Medical sciences ; Molecular Structure ; NAPA ; PABA ; Pharmacology. Drug treatments ; Procaine ; Procaine - analysis ; Procaine - chemistry ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Reproducibility of Results ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-03, Vol.847 (2), p.224-230</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-8c581a936f1cc2137f2f86e87945cbfbe1761ca00b151c85421e507dcaf688243</citedby><cites>FETCH-LOGICAL-c422t-8c581a936f1cc2137f2f86e87945cbfbe1761ca00b151c85421e507dcaf688243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023206008178$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18579026$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17070118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dhananjeyan, Mugunthu R.</creatorcontrib><creatorcontrib>Bykowski, Crystal</creatorcontrib><creatorcontrib>Trendel, Jill A.</creatorcontrib><creatorcontrib>Sarver, Jeffrey G.</creatorcontrib><creatorcontrib>Ando, Howard</creatorcontrib><creatorcontrib>Erhardt, Paul W.</creatorcontrib><title>Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A sensitive high performance liquid chromatography tandem mass spectrometry (LC–MS/MS) method has been developed for simultaneous determination of procaine and its metabolite
p-aminobenzoic acid (PABA).
N-Acetylprocainamide (NAPA) was used as an internal standard for procaine and PABA analysis. This assay method has also been validated in terms of linearity, lower limit of detection, lower limit of quantitation, accuracy and precision as per ICH guidelines. Chromatography was carried out on an XTerra™ MS C
18 column and mass spectrometric analysis was performed using a Quattro Micro™ mass spectrometer working with electro-spray ionization (ESI) source in the positive ion mode. Enhanced selectivity was achieved using multiple reaction monitoring (MRM) functions,
m/
z 237
→
100,
m/
z 138
→
120, and
m/
z 278
→
205 for procaine, PABA and NAPA, respectively. Retention times for PABA, procaine and NAPA were 4.0, 4.7 and 5.8
min, respectively. Linearity for each calibration curve was observed across a range from 100
nM to 5000
nM for PABA, and from 10
nM to 5000
nM for procaine. The intra- and inter-day relative standard deviations (RSD) were <5%.</description><subject>4-Aminobenzoic Acid - analysis</subject><subject>4-Aminobenzoic Acid - chemistry</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Diseases of the skin. Cosmetics</subject><subject>Esters</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>LC–MS/MS</subject><subject>Local anesthetics</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>NAPA</subject><subject>PABA</subject><subject>Pharmacology. Drug treatments</subject><subject>Procaine</subject><subject>Procaine - analysis</subject><subject>Procaine - chemistry</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Reproducibility of Results</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1qGzEQgEVIadKkj5CiS3pbZ6T9kXwKwaQ_4NCD29Kb0M6OiMzuypXWhfTUd-gb9kki44Uce5ph9M1o5mPsSsBCgGhutostPsYwtAsJ0OTaAqA6YedCq7IoVfPjNOe1ggJkKc_Ym5S2AEKBKl-zs0MEIfQ5-77xw76f7Ehhn3hHE8XBj3byYeTB8V0MaP1I3I4d39loC5ufQ0vj7-CRW_Qdb5_4evXvz9-Hzc3Dhg80PYbukr1ytk_0do4X7NuH-6-rT8X6y8fPq7t1gZWUU6Gx1sIuy8YJRClK5aTTDWm1rGpsXUtCNQItQCtqgbqupKAaVIfWNVrLqrxg749z86I_95QmM_iE1PfHg4zK15dS6QzWRxBjSCmSM7voBxufjABzEGq2ZhZqDkIP5Sw0972bP9i3A3UvXbPBDFzPgE1oexftiD69cLpWS5BN5m6PHGUdvzxFk9DTiNT5SDiZLvj_rPIM1T2XAA</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Dhananjeyan, Mugunthu R.</creator><creator>Bykowski, Crystal</creator><creator>Trendel, Jill A.</creator><creator>Sarver, Jeffrey G.</creator><creator>Ando, Howard</creator><creator>Erhardt, Paul W.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method</title><author>Dhananjeyan, Mugunthu R. ; Bykowski, Crystal ; Trendel, Jill A. ; Sarver, Jeffrey G. ; Ando, Howard ; Erhardt, Paul W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-8c581a936f1cc2137f2f86e87945cbfbe1761ca00b151c85421e507dcaf688243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>4-Aminobenzoic Acid - analysis</topic><topic>4-Aminobenzoic Acid - chemistry</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Diseases of the skin. Cosmetics</topic><topic>Esters</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>LC–MS/MS</topic><topic>Local anesthetics</topic><topic>Medical sciences</topic><topic>Molecular Structure</topic><topic>NAPA</topic><topic>PABA</topic><topic>Pharmacology. Drug treatments</topic><topic>Procaine</topic><topic>Procaine - analysis</topic><topic>Procaine - chemistry</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Reproducibility of Results</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dhananjeyan, Mugunthu R.</creatorcontrib><creatorcontrib>Bykowski, Crystal</creatorcontrib><creatorcontrib>Trendel, Jill A.</creatorcontrib><creatorcontrib>Sarver, Jeffrey G.</creatorcontrib><creatorcontrib>Ando, Howard</creatorcontrib><creatorcontrib>Erhardt, Paul W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dhananjeyan, Mugunthu R.</au><au>Bykowski, Crystal</au><au>Trendel, Jill A.</au><au>Sarver, Jeffrey G.</au><au>Ando, Howard</au><au>Erhardt, Paul W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>847</volume><issue>2</issue><spage>224</spage><epage>230</epage><pages>224-230</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A sensitive high performance liquid chromatography tandem mass spectrometry (LC–MS/MS) method has been developed for simultaneous determination of procaine and its metabolite
p-aminobenzoic acid (PABA).
N-Acetylprocainamide (NAPA) was used as an internal standard for procaine and PABA analysis. This assay method has also been validated in terms of linearity, lower limit of detection, lower limit of quantitation, accuracy and precision as per ICH guidelines. Chromatography was carried out on an XTerra™ MS C
18 column and mass spectrometric analysis was performed using a Quattro Micro™ mass spectrometer working with electro-spray ionization (ESI) source in the positive ion mode. Enhanced selectivity was achieved using multiple reaction monitoring (MRM) functions,
m/
z 237
→
100,
m/
z 138
→
120, and
m/
z 278
→
205 for procaine, PABA and NAPA, respectively. Retention times for PABA, procaine and NAPA were 4.0, 4.7 and 5.8
min, respectively. Linearity for each calibration curve was observed across a range from 100
nM to 5000
nM for PABA, and from 10
nM to 5000
nM for procaine. The intra- and inter-day relative standard deviations (RSD) were <5%.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17070118</pmid><doi>10.1016/j.jchromb.2006.10.004</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-03, Vol.847 (2), p.224-230 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70233278 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 4-Aminobenzoic Acid - analysis 4-Aminobenzoic Acid - chemistry Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Diseases of the skin. Cosmetics Esters Fundamental and applied biological sciences. Psychology General pharmacology LC–MS/MS Local anesthetics Medical sciences Molecular Structure NAPA PABA Pharmacology. Drug treatments Procaine Procaine - analysis Procaine - chemistry Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Reproducibility of Results Tandem Mass Spectrometry - methods |
| title | Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method |
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