Aeromonas bivalvium sp. nov., isolated from bivalve molluscs
Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain Correspondence J. Gaspar Lorén jgloren{at}ub.edu A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and...
Gespeichert in:
| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2007-03, Vol.57 (3), p.582-587 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 587 |
|---|---|
| container_issue | 3 |
| container_start_page | 582 |
| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 57 |
| creator | Minana-Galbis, David Farfan, Maribel Fuste, M. Carme Loren, J. Gaspar |
| description | Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Correspondence J. Gaspar Lorén jgloren{at}ub.edu
A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and 868E T , isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus Aeromonas . Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E T clustered together with a similarity of 78 % but did not cluster with any of the Aeromonas genomospecies. DNADNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related Aeromonas genomospecies (3044 %). Useful tests for the phenotypic differentiation of strains 665N and 868E T from other mesophilic Aeromonas species included those for gas from glucose, lysine decarboxylase, VogesProskauer reaction, acid from L -arabinose, hydrolysis of aesculin and utilization of L -lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E T are considered to represent a novel species of the genus Aeromonas , for which the name Aeromonas bivalvium sp. nov. is proposed. The type strain is 868E T (=CECT 7113 T =LMG 23376 T ).
Abbreviations: FAFLP, fluorescence amplified fragment length polymorphism; HG, hybridization group
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 868E T and 665N are DQ504429 and DQ504430 , respectively.
A table giving levels of DNADNA hybridization between strains 868E T and 665N and strains of phylogenetically related Aeromonas species is available as supplementary material in IJSEM Online. |
| doi_str_mv | 10.1099/ijs.0.64497-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70225572</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70225572</sourcerecordid><originalsourceid>FETCH-LOGICAL-c489t-8045554040c7a0c51d01f3327a79cdb080ba025d558c45de99e6c256e786d18a3</originalsourceid><addsrcrecordid>eNqF0D1PwzAQBmALgWgpjKwoC7CQcHb8KbEgxJdUiQVmy3GcYpTEJW6K-PekNKIj051Oj-5OL0KnGDIMSl37j5hBxilVIoU9NMWU85RBTvf_esIn6CjGD4BhAHCIJljkRAmppujm1nWhCa2JSeHXpl77vkniMkvasM6uEh9DbVauTKpBjcIlTajrPtp4jA4qU0d3MtYZenu4f717Sucvj893t_PUUqlWqQTKGKNAwQoDluEScJXnRBihbFmAhMIAYSVj0lJWOqUct4RxJyQvsTT5DF1s9y678Nm7uNKNj9bVtWld6KMWQAhjgvwLsWI851gOMN1C24UYO1fpZecb031rDHqTqx5y1aB_c9Uw-LNxcV80rtzpMcgBnI_ARGvqqjOt9XHnJFOE482Hl1v37hfvX75zeuHaxg9vFD5sjjKhc80kyX8AOwSMww</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19563618</pqid></control><display><type>article</type><title>Aeromonas bivalvium sp. nov., isolated from bivalve molluscs</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Minana-Galbis, David ; Farfan, Maribel ; Fuste, M. Carme ; Loren, J. Gaspar</creator><creatorcontrib>Minana-Galbis, David ; Farfan, Maribel ; Fuste, M. Carme ; Loren, J. Gaspar</creatorcontrib><description>Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Correspondence J. Gaspar Lorén jgloren{at}ub.edu
A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and 868E T , isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus Aeromonas . Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E T clustered together with a similarity of 78 % but did not cluster with any of the Aeromonas genomospecies. DNADNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related Aeromonas genomospecies (3044 %). Useful tests for the phenotypic differentiation of strains 665N and 868E T from other mesophilic Aeromonas species included those for gas from glucose, lysine decarboxylase, VogesProskauer reaction, acid from L -arabinose, hydrolysis of aesculin and utilization of L -lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E T are considered to represent a novel species of the genus Aeromonas , for which the name Aeromonas bivalvium sp. nov. is proposed. The type strain is 868E T (=CECT 7113 T =LMG 23376 T ).
Abbreviations: FAFLP, fluorescence amplified fragment length polymorphism; HG, hybridization group
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 868E T and 665N are DQ504429 and DQ504430 , respectively.
A table giving levels of DNADNA hybridization between strains 868E T and 665N and strains of phylogenetically related Aeromonas species is available as supplementary material in IJSEM Online.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.64497-0</identifier><identifier>PMID: 17329789</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Aeromonas ; Aeromonas - classification ; Aeromonas - genetics ; Aeromonas - isolation & purification ; Aeromonas bivalvium ; Animals ; Bacteriology ; Base Composition ; Biological and medical sciences ; Bivalvia ; Bivalvia - microbiology ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Molecular Sequence Data ; Phylogeny ; Polymorphism, Restriction Fragment Length ; RNA, Ribosomal, 16S - genetics ; Systematics</subject><ispartof>International journal of systematic and evolutionary microbiology, 2007-03, Vol.57 (3), p.582-587</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-8045554040c7a0c51d01f3327a79cdb080ba025d558c45de99e6c256e786d18a3</citedby><cites>FETCH-LOGICAL-c489t-8045554040c7a0c51d01f3327a79cdb080ba025d558c45de99e6c256e786d18a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18592612$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17329789$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minana-Galbis, David</creatorcontrib><creatorcontrib>Farfan, Maribel</creatorcontrib><creatorcontrib>Fuste, M. Carme</creatorcontrib><creatorcontrib>Loren, J. Gaspar</creatorcontrib><title>Aeromonas bivalvium sp. nov., isolated from bivalve molluscs</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Correspondence J. Gaspar Lorén jgloren{at}ub.edu
A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and 868E T , isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus Aeromonas . Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E T clustered together with a similarity of 78 % but did not cluster with any of the Aeromonas genomospecies. DNADNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related Aeromonas genomospecies (3044 %). Useful tests for the phenotypic differentiation of strains 665N and 868E T from other mesophilic Aeromonas species included those for gas from glucose, lysine decarboxylase, VogesProskauer reaction, acid from L -arabinose, hydrolysis of aesculin and utilization of L -lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E T are considered to represent a novel species of the genus Aeromonas , for which the name Aeromonas bivalvium sp. nov. is proposed. The type strain is 868E T (=CECT 7113 T =LMG 23376 T ).
Abbreviations: FAFLP, fluorescence amplified fragment length polymorphism; HG, hybridization group
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 868E T and 665N are DQ504429 and DQ504430 , respectively.
A table giving levels of DNADNA hybridization between strains 868E T and 665N and strains of phylogenetically related Aeromonas species is available as supplementary material in IJSEM Online.</description><subject>Aeromonas</subject><subject>Aeromonas - classification</subject><subject>Aeromonas - genetics</subject><subject>Aeromonas - isolation & purification</subject><subject>Aeromonas bivalvium</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>Bivalvia</subject><subject>Bivalvia - microbiology</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Systematics</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0D1PwzAQBmALgWgpjKwoC7CQcHb8KbEgxJdUiQVmy3GcYpTEJW6K-PekNKIj051Oj-5OL0KnGDIMSl37j5hBxilVIoU9NMWU85RBTvf_esIn6CjGD4BhAHCIJljkRAmppujm1nWhCa2JSeHXpl77vkniMkvasM6uEh9DbVauTKpBjcIlTajrPtp4jA4qU0d3MtYZenu4f717Sucvj893t_PUUqlWqQTKGKNAwQoDluEScJXnRBihbFmAhMIAYSVj0lJWOqUct4RxJyQvsTT5DF1s9y678Nm7uNKNj9bVtWld6KMWQAhjgvwLsWI851gOMN1C24UYO1fpZecb031rDHqTqx5y1aB_c9Uw-LNxcV80rtzpMcgBnI_ARGvqqjOt9XHnJFOE482Hl1v37hfvX75zeuHaxg9vFD5sjjKhc80kyX8AOwSMww</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Minana-Galbis, David</creator><creator>Farfan, Maribel</creator><creator>Fuste, M. Carme</creator><creator>Loren, J. Gaspar</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>Aeromonas bivalvium sp. nov., isolated from bivalve molluscs</title><author>Minana-Galbis, David ; Farfan, Maribel ; Fuste, M. Carme ; Loren, J. Gaspar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-8045554040c7a0c51d01f3327a79cdb080ba025d558c45de99e6c256e786d18a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aeromonas</topic><topic>Aeromonas - classification</topic><topic>Aeromonas - genetics</topic><topic>Aeromonas - isolation & purification</topic><topic>Aeromonas bivalvium</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Base Composition</topic><topic>Biological and medical sciences</topic><topic>Bivalvia</topic><topic>Bivalvia - microbiology</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Systematics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minana-Galbis, David</creatorcontrib><creatorcontrib>Farfan, Maribel</creatorcontrib><creatorcontrib>Fuste, M. Carme</creatorcontrib><creatorcontrib>Loren, J. Gaspar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minana-Galbis, David</au><au>Farfan, Maribel</au><au>Fuste, M. Carme</au><au>Loren, J. Gaspar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aeromonas bivalvium sp. nov., isolated from bivalve molluscs</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>57</volume><issue>3</issue><spage>582</spage><epage>587</epage><pages>582-587</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Correspondence J. Gaspar Lorén jgloren{at}ub.edu
A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and 868E T , isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus Aeromonas . Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E T clustered together with a similarity of 78 % but did not cluster with any of the Aeromonas genomospecies. DNADNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related Aeromonas genomospecies (3044 %). Useful tests for the phenotypic differentiation of strains 665N and 868E T from other mesophilic Aeromonas species included those for gas from glucose, lysine decarboxylase, VogesProskauer reaction, acid from L -arabinose, hydrolysis of aesculin and utilization of L -lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E T are considered to represent a novel species of the genus Aeromonas , for which the name Aeromonas bivalvium sp. nov. is proposed. The type strain is 868E T (=CECT 7113 T =LMG 23376 T ).
Abbreviations: FAFLP, fluorescence amplified fragment length polymorphism; HG, hybridization group
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 868E T and 665N are DQ504429 and DQ504430 , respectively.
A table giving levels of DNADNA hybridization between strains 868E T and 665N and strains of phylogenetically related Aeromonas species is available as supplementary material in IJSEM Online.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17329789</pmid><doi>10.1099/ijs.0.64497-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2007-03, Vol.57 (3), p.582-587 |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70225572 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aeromonas Aeromonas - classification Aeromonas - genetics Aeromonas - isolation & purification Aeromonas bivalvium Animals Bacteriology Base Composition Biological and medical sciences Bivalvia Bivalvia - microbiology DNA, Bacterial - genetics DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Microbiology Molecular Sequence Data Phylogeny Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Systematics |
| title | Aeromonas bivalvium sp. nov., isolated from bivalve molluscs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T11%3A55%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aeromonas%20bivalvium%20sp.%20nov.,%20isolated%20from%20bivalve%20molluscs&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Minana-Galbis,%20David&rft.date=2007-03-01&rft.volume=57&rft.issue=3&rft.spage=582&rft.epage=587&rft.pages=582-587&rft.issn=1466-5026&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijs.0.64497-0&rft_dat=%3Cproquest_cross%3E70225572%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19563618&rft_id=info:pmid/17329789&rfr_iscdi=true |