A modified peptide mapping strategy for quantifying site-specific deamidation by electrospray time-of-flight mass spectrometry

A modified peptide mapping strategy using electrospray time‐of‐flight mass spectrometry with high‐performance liquid chromatography (HPLC/MS) provides an improved measure of deamidation by performing proteolytic digestion at low temperature (4°C), low pH (6.0) and in organic solvent (≥10% acetonitri...

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Veröffentlicht in:	Rapid communications in mass spectrometry 2007-01, Vol.21 (6), p.830-836
1. Verfasser:	Stroop, Steven D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Amides - chemistry Amino Acids - chemistry Chromatography, High Pressure Liquid - methods Peptide Mapping - methods Peptides - chemistry Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
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