IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY
SUMMARY 1 Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel. 2 The PhD‐C7C phage display pept...
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| Veröffentlicht in: | Clinical and experimental pharmacology & physiology 2007-04, Vol.34 (4), p.286-289 |
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| container_title | Clinical and experimental pharmacology & physiology |
| container_volume | 34 |
| creator | Takagi, Tamaki Arisawa, Tomiyasu Yamamoto, Kazumasa Hirata, Ichiro Nakano, Hiroshi Sawada, Makoto |
| description | SUMMARY
1
Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel.
2
The PhD‐C7C phage display peptide library was used for biopanning against mouse isolated bowel, either untreated (control) or with inflammation caused by ischaemia–reperfusion injury. One hundred clones were selected from among those obtained by two biopanning procedures and the amino acid sequences of these clones were identified by determination of the base sequences.
3
Then, 20 clones were selected by an alignment process, after which the three clones with the highest affinity for inflammatory bowel were identified. One of these three clones had significantly higher affinity for inflammatory bowel than for normal bowel.
4
In conclusion, biopanning against isolated bowel samples identified an amino acid sequence (SQSHPRH) with a specific high affinity for inflammatory bowel. |
| doi_str_mv | 10.1111/j.1440-1681.2007.04563.x |
| format | Article |
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1
Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel.
2
The PhD‐C7C phage display peptide library was used for biopanning against mouse isolated bowel, either untreated (control) or with inflammation caused by ischaemia–reperfusion injury. One hundred clones were selected from among those obtained by two biopanning procedures and the amino acid sequences of these clones were identified by determination of the base sequences.
3
Then, 20 clones were selected by an alignment process, after which the three clones with the highest affinity for inflammatory bowel were identified. One of these three clones had significantly higher affinity for inflammatory bowel than for normal bowel.
4
In conclusion, biopanning against isolated bowel samples identified an amino acid sequence (SQSHPRH) with a specific high affinity for inflammatory bowel.</description><identifier>ISSN: 0305-1870</identifier><identifier>EISSN: 1440-1681</identifier><identifier>DOI: 10.1111/j.1440-1681.2007.04563.x</identifier><identifier>PMID: 17324139</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Adsorption ; Amino Acid Sequence ; Animals ; Bacteriophages - genetics ; Binding Sites ; Binding, Competitive ; inflammatory bowel ; Inflammatory Bowel Diseases - genetics ; Inflammatory Bowel Diseases - metabolism ; Inflammatory Bowel Diseases - pathology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Ligands ; Male ; Mice ; Mice, Inbred C57BL ; Oligopeptides - chemistry ; Oligopeptides - genetics ; Oligopeptides - metabolism ; Peptide Library ; phage display ; PhD-C7C phage ; Reperfusion Injury - physiopathology ; targeting ligands</subject><ispartof>Clinical and experimental pharmacology & physiology, 2007-04, Vol.34 (4), p.286-289</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4913-689404026d21a129f725ef8c575990bcd8fc73bd4e4b5c7d9a641ca1760f2d1f3</citedby><cites>FETCH-LOGICAL-c4913-689404026d21a129f725ef8c575990bcd8fc73bd4e4b5c7d9a641ca1760f2d1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1681.2007.04563.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1681.2007.04563.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17324139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takagi, Tamaki</creatorcontrib><creatorcontrib>Arisawa, Tomiyasu</creatorcontrib><creatorcontrib>Yamamoto, Kazumasa</creatorcontrib><creatorcontrib>Hirata, Ichiro</creatorcontrib><creatorcontrib>Nakano, Hiroshi</creatorcontrib><creatorcontrib>Sawada, Makoto</creatorcontrib><title>IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY</title><title>Clinical and experimental pharmacology & physiology</title><addtitle>Clin Exp Pharmacol Physiol</addtitle><description>SUMMARY
1
Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel.
2
The PhD‐C7C phage display peptide library was used for biopanning against mouse isolated bowel, either untreated (control) or with inflammation caused by ischaemia–reperfusion injury. One hundred clones were selected from among those obtained by two biopanning procedures and the amino acid sequences of these clones were identified by determination of the base sequences.
3
Then, 20 clones were selected by an alignment process, after which the three clones with the highest affinity for inflammatory bowel were identified. One of these three clones had significantly higher affinity for inflammatory bowel than for normal bowel.
4
In conclusion, biopanning against isolated bowel samples identified an amino acid sequence (SQSHPRH) with a specific high affinity for inflammatory bowel.</description><subject>Adsorption</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacteriophages - genetics</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>inflammatory bowel</subject><subject>Inflammatory Bowel Diseases - genetics</subject><subject>Inflammatory Bowel Diseases - metabolism</subject><subject>Inflammatory Bowel Diseases - pathology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Ligands</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oligopeptides - chemistry</subject><subject>Oligopeptides - genetics</subject><subject>Oligopeptides - metabolism</subject><subject>Peptide Library</subject><subject>phage display</subject><subject>PhD-C7C phage</subject><subject>Reperfusion Injury - physiopathology</subject><subject>targeting ligands</subject><issn>0305-1870</issn><issn>1440-1681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9vmzAYh61pU5u1_QqTT7vBXv-Hww6MEGqJQFaIppwsAkZKliwtbrT02w-aqDtuvtiWn-dn6fcihAn4ZFhftj7hHDwiA-JTAOUDF5L5p3do8vbwHk2AgfBIoOAafXRuCwACJLtC10QxygkLJ6jV0ySv9EzHUaWLHBcznOk0yqcl_qbzqc5TXC6S-BXIshWuCqzzWRbN51FVPKyGS5WUlc6jDM-XcVFGeFmO0uI-ShM81eUii1a36ENX75y9u-w3aDlLqvjey4p0zPUaHhLmySDkwIHKlpKa0LBTVNguaIQSYQjrpg26RrF1yy1fi0a1YS05aWqiJHS0JR27QZ_PuY_94elo3bPZb1xjd7v6lz0cnVFACQjB_wlS4ExyKQYwOINNf3Cut5157Df7un8xBMw4CrM1Y-NmbNyMozCvozCnQf10-eO43tv2r3jpfgC-noHfm519-e9gEyeL8TT43tnfuGd7evPr_qeRiilhfuSpKb8HXMqSmYz9AXvunJ8</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Takagi, Tamaki</creator><creator>Arisawa, Tomiyasu</creator><creator>Yamamoto, Kazumasa</creator><creator>Hirata, Ichiro</creator><creator>Nakano, Hiroshi</creator><creator>Sawada, Makoto</creator><general>Blackwell Publishing Asia</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY</title><author>Takagi, Tamaki ; Arisawa, Tomiyasu ; Yamamoto, Kazumasa ; Hirata, Ichiro ; Nakano, Hiroshi ; Sawada, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4913-689404026d21a129f725ef8c575990bcd8fc73bd4e4b5c7d9a641ca1760f2d1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adsorption</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacteriophages - genetics</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>inflammatory bowel</topic><topic>Inflammatory Bowel Diseases - genetics</topic><topic>Inflammatory Bowel Diseases - metabolism</topic><topic>Inflammatory Bowel Diseases - pathology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Ligands</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oligopeptides - chemistry</topic><topic>Oligopeptides - genetics</topic><topic>Oligopeptides - metabolism</topic><topic>Peptide Library</topic><topic>phage display</topic><topic>PhD-C7C phage</topic><topic>Reperfusion Injury - physiopathology</topic><topic>targeting ligands</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takagi, Tamaki</creatorcontrib><creatorcontrib>Arisawa, Tomiyasu</creatorcontrib><creatorcontrib>Yamamoto, Kazumasa</creatorcontrib><creatorcontrib>Hirata, Ichiro</creatorcontrib><creatorcontrib>Nakano, Hiroshi</creatorcontrib><creatorcontrib>Sawada, Makoto</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental pharmacology & physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takagi, Tamaki</au><au>Arisawa, Tomiyasu</au><au>Yamamoto, Kazumasa</au><au>Hirata, Ichiro</au><au>Nakano, Hiroshi</au><au>Sawada, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY</atitle><jtitle>Clinical and experimental pharmacology & physiology</jtitle><addtitle>Clin Exp Pharmacol Physiol</addtitle><date>2007-04</date><risdate>2007</risdate><volume>34</volume><issue>4</issue><spage>286</spage><epage>289</epage><pages>286-289</pages><issn>0305-1870</issn><eissn>1440-1681</eissn><abstract>SUMMARY
1
Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel.
2
The PhD‐C7C phage display peptide library was used for biopanning against mouse isolated bowel, either untreated (control) or with inflammation caused by ischaemia–reperfusion injury. One hundred clones were selected from among those obtained by two biopanning procedures and the amino acid sequences of these clones were identified by determination of the base sequences.
3
Then, 20 clones were selected by an alignment process, after which the three clones with the highest affinity for inflammatory bowel were identified. One of these three clones had significantly higher affinity for inflammatory bowel than for normal bowel.
4
In conclusion, biopanning against isolated bowel samples identified an amino acid sequence (SQSHPRH) with a specific high affinity for inflammatory bowel.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>17324139</pmid><doi>10.1111/j.1440-1681.2007.04563.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | Clinical and experimental pharmacology & physiology, 2007-04, Vol.34 (4), p.286-289 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adsorption Amino Acid Sequence Animals Bacteriophages - genetics Binding Sites Binding, Competitive inflammatory bowel Inflammatory Bowel Diseases - genetics Inflammatory Bowel Diseases - metabolism Inflammatory Bowel Diseases - pathology Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Ligands Male Mice Mice, Inbred C57BL Oligopeptides - chemistry Oligopeptides - genetics Oligopeptides - metabolism Peptide Library phage display PhD-C7C phage Reperfusion Injury - physiopathology targeting ligands |
| title | IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY |
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