A proteomic study of serum from children with autism showing differential expression of apolipoproteins and complement proteins
Modern methods that use systematic, quantitative and unbiased approaches are making it possible to discover proteins altered by a disease. To identify proteins that might be differentially expressed in autism, serum proteins from blood were subjected to trypsin digestion followed by liquid chromatog...
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| Veröffentlicht in: | Molecular psychiatry 2007-03, Vol.12 (3), p.292-306 |
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| container_title | Molecular psychiatry |
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| creator | Corbett, B A Kantor, A B Schulman, H Walker, W L Lit, L Ashwood, P Rocke, D M Sharp, F R |
| description | Modern methods that use systematic, quantitative and unbiased approaches are making it possible to discover proteins altered by a disease. To identify proteins that might be differentially expressed in autism, serum proteins from blood were subjected to trypsin digestion followed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) on time-of-flight (TOF) instruments to identify differentially expressed peptides. Children with autism 4–6 years of age (
n
=69) were compared to typically developing children (
n
=35) with similar age and gender distributions. A total of 6348 peptide components were quantified. Of these, five peptide components corresponding to four known proteins had an effect size >0.99 with a
P |
| doi_str_mv | 10.1038/sj.mp.4001943 |
| format | Article |
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n
=69) were compared to typically developing children (
n
=35) with similar age and gender distributions. A total of 6348 peptide components were quantified. Of these, five peptide components corresponding to four known proteins had an effect size >0.99 with a
P
<0.05 and a Mascot identification score of 30 or greater for autism compared to controls. The four proteins were: Apolipoprotein (apo) B-100, Complement Factor H Related Protein (FHR1), Complement C1q and Fibronectin 1 (FN1). In addition, apo B-100 and apo A-IV were higher in children with high compared to low functioning autism. Apos are involved in the transport of lipids, cholesterol and vitamin E. The complement system is involved in the lysis and removal of infectious organisms in blood, and may be involved in cellular apoptosis in brain. Despite limitations of the study, including the low fold changes and variable detection rates for the peptide components, the data support possible differences of circulating proteins in autism, and should help stimulate the continued search for causes and treatments of autism by examining peripheral blood.</description><identifier>ISSN: 1359-4184</identifier><identifier>EISSN: 1476-5578</identifier><identifier>DOI: 10.1038/sj.mp.4001943</identifier><identifier>PMID: 17189958</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Apolipoproteins ; Apolipoproteins - blood ; Apoptosis ; Autism ; Autistic children ; Autistic Disorder - blood ; Behavioral Sciences ; Biological and medical sciences ; Biological Psychology ; Child ; Child clinical studies ; Child, Preschool ; Children ; Cholesterol ; Complement component C1q ; Complement factor H ; Complement system ; Complement System Proteins - metabolism ; Developmental disorders ; Female ; Fibronectin ; Gene Expression - physiology ; Humans ; Infantile autism ; Lipids ; Liquid chromatography ; Lysis ; Male ; Mass Spectrometry - methods ; Mass spectroscopy ; Medical sciences ; Medicine ; Medicine & Public Health ; Neurosciences ; original-article ; Peptides ; Peripheral blood ; Pharmacotherapy ; Proteins ; Proteomics ; Proteomics - methods ; Psychiatry ; Psychology. Psychoanalysis. Psychiatry ; Psychopathology. Psychiatry ; Serum proteins ; Statistics, Nonparametric ; Trypsin ; Vitamin E</subject><ispartof>Molecular psychiatry, 2007-03, Vol.12 (3), p.292-306</ispartof><rights>Springer Nature Limited 2007</rights><rights>2007 INIST-CNRS</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2007</rights><rights>Nature Publishing Group 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-82d74c898ec327a101603f9699b35c0d46be331cfc44d9f1a4508f2ed736261e3</citedby><cites>FETCH-LOGICAL-c513t-82d74c898ec327a101603f9699b35c0d46be331cfc44d9f1a4508f2ed736261e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/sj.mp.4001943$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/sj.mp.4001943$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18536314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17189958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corbett, B A</creatorcontrib><creatorcontrib>Kantor, A B</creatorcontrib><creatorcontrib>Schulman, H</creatorcontrib><creatorcontrib>Walker, W L</creatorcontrib><creatorcontrib>Lit, L</creatorcontrib><creatorcontrib>Ashwood, P</creatorcontrib><creatorcontrib>Rocke, D M</creatorcontrib><creatorcontrib>Sharp, F R</creatorcontrib><title>A proteomic study of serum from children with autism showing differential expression of apolipoproteins and complement proteins</title><title>Molecular psychiatry</title><addtitle>Mol Psychiatry</addtitle><addtitle>Mol Psychiatry</addtitle><description>Modern methods that use systematic, quantitative and unbiased approaches are making it possible to discover proteins altered by a disease. To identify proteins that might be differentially expressed in autism, serum proteins from blood were subjected to trypsin digestion followed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) on time-of-flight (TOF) instruments to identify differentially expressed peptides. Children with autism 4–6 years of age (
n
=69) were compared to typically developing children (
n
=35) with similar age and gender distributions. A total of 6348 peptide components were quantified. Of these, five peptide components corresponding to four known proteins had an effect size >0.99 with a
P
<0.05 and a Mascot identification score of 30 or greater for autism compared to controls. The four proteins were: Apolipoprotein (apo) B-100, Complement Factor H Related Protein (FHR1), Complement C1q and Fibronectin 1 (FN1). In addition, apo B-100 and apo A-IV were higher in children with high compared to low functioning autism. Apos are involved in the transport of lipids, cholesterol and vitamin E. The complement system is involved in the lysis and removal of infectious organisms in blood, and may be involved in cellular apoptosis in brain. Despite limitations of the study, including the low fold changes and variable detection rates for the peptide components, the data support possible differences of circulating proteins in autism, and should help stimulate the continued search for causes and treatments of autism by examining peripheral blood.</description><subject>Apolipoproteins</subject><subject>Apolipoproteins - blood</subject><subject>Apoptosis</subject><subject>Autism</subject><subject>Autistic children</subject><subject>Autistic Disorder - blood</subject><subject>Behavioral Sciences</subject><subject>Biological and medical sciences</subject><subject>Biological Psychology</subject><subject>Child</subject><subject>Child clinical studies</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Cholesterol</subject><subject>Complement component C1q</subject><subject>Complement factor H</subject><subject>Complement system</subject><subject>Complement System Proteins - metabolism</subject><subject>Developmental disorders</subject><subject>Female</subject><subject>Fibronectin</subject><subject>Gene Expression - physiology</subject><subject>Humans</subject><subject>Infantile autism</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Lysis</subject><subject>Male</subject><subject>Mass Spectrometry - methods</subject><subject>Mass spectroscopy</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neurosciences</subject><subject>original-article</subject><subject>Peptides</subject><subject>Peripheral blood</subject><subject>Pharmacotherapy</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Psychiatry</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopathology. Psychiatry</subject><subject>Serum proteins</subject><subject>Statistics, Nonparametric</subject><subject>Trypsin</subject><subject>Vitamin E</subject><issn>1359-4184</issn><issn>1476-5578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkk2LFDEQhhtR3HX16FWCorceU52PTo7DsqvCghc9h0w6mcnQSdqkm3VP_nUz7sigrEgOCVVPvW8VqaZ5CXgFmIj3Zb8K04piDJKSR8050J63jPXicX0TJlsKgp41z0rZV6Ym2dPmDHoQUjJx3vxYoymn2abgDSrzMtyh5FCxeQnI5RSQ2flxyDaiWz_vkF5mXwIqu3Tr4xYN3jlbk7PXI7Lfp2xL8SkeJPSURj-lX-I-FqTjgEwK02hD5dHv-PPmidNjsS-O90Xz9frqy-XH9ubzh0-X65vWMCBzK7qhp0ZIYQ3peg0YOCZOcik3hBk8UL6xhIBxhtJBOtCUYeE6O_SEdxwsuWje3etW42-LLbMKvhg7jjratBTV4w5Lgdl_QZAcAGNewTd_gfu05FiHUB2nrGekk6JSr_9JdQCcdYScpLZ6tMpHl-aszcFXrUEIRnsMh85WD1D1DLZ-XorW-Rr_o6C9LzA5lZKtU1P2Qec7BVgdlkeVvQqTOi5P5V8de102wQ4n-rgtFXh7BHQxenRZR-PLiROMcAL01Gmpqbi1-TT0w84_AXA92-M</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Corbett, B A</creator><creator>Kantor, A B</creator><creator>Schulman, H</creator><creator>Walker, W L</creator><creator>Lit, L</creator><creator>Ashwood, P</creator><creator>Rocke, D M</creator><creator>Sharp, F R</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>A proteomic study of serum from children with autism showing differential expression of apolipoproteins and complement proteins</title><author>Corbett, B A ; Kantor, A B ; Schulman, H ; Walker, W L ; Lit, L ; Ashwood, P ; Rocke, D M ; Sharp, F R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-82d74c898ec327a101603f9699b35c0d46be331cfc44d9f1a4508f2ed736261e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Apolipoproteins</topic><topic>Apolipoproteins - blood</topic><topic>Apoptosis</topic><topic>Autism</topic><topic>Autistic children</topic><topic>Autistic Disorder - blood</topic><topic>Behavioral Sciences</topic><topic>Biological and medical sciences</topic><topic>Biological Psychology</topic><topic>Child</topic><topic>Child clinical studies</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Cholesterol</topic><topic>Complement component C1q</topic><topic>Complement factor H</topic><topic>Complement system</topic><topic>Complement System Proteins - metabolism</topic><topic>Developmental disorders</topic><topic>Female</topic><topic>Fibronectin</topic><topic>Gene Expression - physiology</topic><topic>Humans</topic><topic>Infantile autism</topic><topic>Lipids</topic><topic>Liquid chromatography</topic><topic>Lysis</topic><topic>Male</topic><topic>Mass Spectrometry - methods</topic><topic>Mass spectroscopy</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neurosciences</topic><topic>original-article</topic><topic>Peptides</topic><topic>Peripheral blood</topic><topic>Pharmacotherapy</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>Psychiatry</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopathology. Psychiatry</topic><topic>Serum proteins</topic><topic>Statistics, Nonparametric</topic><topic>Trypsin</topic><topic>Vitamin E</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Corbett, B A</creatorcontrib><creatorcontrib>Kantor, A B</creatorcontrib><creatorcontrib>Schulman, H</creatorcontrib><creatorcontrib>Walker, W L</creatorcontrib><creatorcontrib>Lit, L</creatorcontrib><creatorcontrib>Ashwood, P</creatorcontrib><creatorcontrib>Rocke, D M</creatorcontrib><creatorcontrib>Sharp, F R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular psychiatry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Corbett, B A</au><au>Kantor, A B</au><au>Schulman, H</au><au>Walker, W L</au><au>Lit, L</au><au>Ashwood, P</au><au>Rocke, D M</au><au>Sharp, F R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A proteomic study of serum from children with autism showing differential expression of apolipoproteins and complement proteins</atitle><jtitle>Molecular psychiatry</jtitle><stitle>Mol Psychiatry</stitle><addtitle>Mol Psychiatry</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>12</volume><issue>3</issue><spage>292</spage><epage>306</epage><pages>292-306</pages><issn>1359-4184</issn><eissn>1476-5578</eissn><abstract>Modern methods that use systematic, quantitative and unbiased approaches are making it possible to discover proteins altered by a disease. To identify proteins that might be differentially expressed in autism, serum proteins from blood were subjected to trypsin digestion followed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) on time-of-flight (TOF) instruments to identify differentially expressed peptides. Children with autism 4–6 years of age (
n
=69) were compared to typically developing children (
n
=35) with similar age and gender distributions. A total of 6348 peptide components were quantified. Of these, five peptide components corresponding to four known proteins had an effect size >0.99 with a
P
<0.05 and a Mascot identification score of 30 or greater for autism compared to controls. The four proteins were: Apolipoprotein (apo) B-100, Complement Factor H Related Protein (FHR1), Complement C1q and Fibronectin 1 (FN1). In addition, apo B-100 and apo A-IV were higher in children with high compared to low functioning autism. Apos are involved in the transport of lipids, cholesterol and vitamin E. The complement system is involved in the lysis and removal of infectious organisms in blood, and may be involved in cellular apoptosis in brain. Despite limitations of the study, including the low fold changes and variable detection rates for the peptide components, the data support possible differences of circulating proteins in autism, and should help stimulate the continued search for causes and treatments of autism by examining peripheral blood.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>17189958</pmid><doi>10.1038/sj.mp.4001943</doi><tpages>15</tpages></addata></record> |
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| subjects | Apolipoproteins Apolipoproteins - blood Apoptosis Autism Autistic children Autistic Disorder - blood Behavioral Sciences Biological and medical sciences Biological Psychology Child Child clinical studies Child, Preschool Children Cholesterol Complement component C1q Complement factor H Complement system Complement System Proteins - metabolism Developmental disorders Female Fibronectin Gene Expression - physiology Humans Infantile autism Lipids Liquid chromatography Lysis Male Mass Spectrometry - methods Mass spectroscopy Medical sciences Medicine Medicine & Public Health Neurosciences original-article Peptides Peripheral blood Pharmacotherapy Proteins Proteomics Proteomics - methods Psychiatry Psychology. Psychoanalysis. Psychiatry Psychopathology. Psychiatry Serum proteins Statistics, Nonparametric Trypsin Vitamin E |
| title | A proteomic study of serum from children with autism showing differential expression of apolipoproteins and complement proteins |
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