Candidate genes involved in enhanced growth of human prostate cancer under high fat feeding identified by microarray analysis
BACKGROUND Several studies have suggested that a high fat diet (HFD) may be a risk factor of prostate cancer (PCa). As a first step to delineate the molecular mechanisms underlying the enhanced progression of PCa under HFD, we investigated the differential gene expressions of a human PCa xenograft u...
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| Veröffentlicht in: | The Prostate 2008-02, Vol.68 (3), p.321-335 |
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| creator | Narita, Shintaro Tsuchiya, Norihiko Saito, Mitsuru Inoue, Takamitsu Kumazawa, Teruaki Yuasa, Takeshi Nakamura, Akira Habuchi, Tomonori |
| description | BACKGROUND
Several studies have suggested that a high fat diet (HFD) may be a risk factor of prostate cancer (PCa). As a first step to delineate the molecular mechanisms underlying the enhanced progression of PCa under HFD, we investigated the differential gene expressions of a human PCa xenograft under HFD and a low fat diet (LFD).
METHODS
LNCaP cells were subcutaneously injected in 20 nude mice, which were equally divided into two groups, the HFD group and LFD group. Oligonucleotide microarray analyses were performed using mice xenografts from HFD and LFD, and the results of candidate genes with a significant differential expression were validated by quantitative RT‐PCR experiments. As for insulin‐like growth factor I receptor (IGF‐IR), protein expression levels were further examined by immunohistochemistry in xenograft tissues and in 78 radical prostatectomy specimens.
RESULTS
Tumor volume and serum PSA levels were significantly higher in the HFD group than in the LFD group (P |
| doi_str_mv | 10.1002/pros.20681 |
| format | Article |
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Several studies have suggested that a high fat diet (HFD) may be a risk factor of prostate cancer (PCa). As a first step to delineate the molecular mechanisms underlying the enhanced progression of PCa under HFD, we investigated the differential gene expressions of a human PCa xenograft under HFD and a low fat diet (LFD).
METHODS
LNCaP cells were subcutaneously injected in 20 nude mice, which were equally divided into two groups, the HFD group and LFD group. Oligonucleotide microarray analyses were performed using mice xenografts from HFD and LFD, and the results of candidate genes with a significant differential expression were validated by quantitative RT‐PCR experiments. As for insulin‐like growth factor I receptor (IGF‐IR), protein expression levels were further examined by immunohistochemistry in xenograft tissues and in 78 radical prostatectomy specimens.
RESULTS
Tumor volume and serum PSA levels were significantly higher in the HFD group than in the LFD group (P < 0.001 and P = 0.006, respectively). We found 64 up‐regulated genes (0.19%) and 14 down‐regulated genes (0.04%) with more than twofold differences in the HFD xenograft. IGF‐IR, TNFRSF, and LPL showed striking differences in the quantitative RT‐PCR experiment. Immunostaining further revealed marked enhanced IGF‐IR expression in the HFD xenograft. In human PCa, the lowest IGF‐IR immunoreactivity group tended to have the lowest body mass index in both normal and PCa epithelium.
CONCLUSION
HFD induced remarkable up‐ and down‐regulation of mRNA of a substantial number of genes. Furthermore, the IGF‐I system may be involved in the HFD‐associated enhanced progression of PCa. Prostate 68: 321–335, 2008. © 2008 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.20681</identifier><identifier>PMID: 18175332</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Dietary Fats - administration & dosage ; Gene Expression Regulation, Neoplastic ; Gynecology. Andrology. Obstetrics ; high fat diet ; Humans ; Immunohistochemistry ; insulin-like growth factor I receptor ; Male ; Male genital diseases ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; microarray ; Nephrology. Urinary tract diseases ; Oligonucleotide Array Sequence Analysis ; prostate cancer ; Prostate-Specific Antigen - blood ; Prostatic Neoplasms - blood ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Receptor, IGF Type 1 - biosynthesis ; Receptor, IGF Type 1 - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Specific Pathogen-Free Organisms ; Tumors ; Tumors of the urinary system ; Urinary tract. Prostate gland</subject><ispartof>The Prostate, 2008-02, Vol.68 (3), p.321-335</ispartof><rights>Copyright © 2008 Wiley‐Liss, Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4971-b5a20b96d9220ef70f2a150c633c0373846cab64ffbe77eaf6b94524addb9ad23</citedby><cites>FETCH-LOGICAL-c4971-b5a20b96d9220ef70f2a150c633c0373846cab64ffbe77eaf6b94524addb9ad23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.20681$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.20681$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20001659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18175332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Narita, Shintaro</creatorcontrib><creatorcontrib>Tsuchiya, Norihiko</creatorcontrib><creatorcontrib>Saito, Mitsuru</creatorcontrib><creatorcontrib>Inoue, Takamitsu</creatorcontrib><creatorcontrib>Kumazawa, Teruaki</creatorcontrib><creatorcontrib>Yuasa, Takeshi</creatorcontrib><creatorcontrib>Nakamura, Akira</creatorcontrib><creatorcontrib>Habuchi, Tomonori</creatorcontrib><title>Candidate genes involved in enhanced growth of human prostate cancer under high fat feeding identified by microarray analysis</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
Several studies have suggested that a high fat diet (HFD) may be a risk factor of prostate cancer (PCa). As a first step to delineate the molecular mechanisms underlying the enhanced progression of PCa under HFD, we investigated the differential gene expressions of a human PCa xenograft under HFD and a low fat diet (LFD).
METHODS
LNCaP cells were subcutaneously injected in 20 nude mice, which were equally divided into two groups, the HFD group and LFD group. Oligonucleotide microarray analyses were performed using mice xenografts from HFD and LFD, and the results of candidate genes with a significant differential expression were validated by quantitative RT‐PCR experiments. As for insulin‐like growth factor I receptor (IGF‐IR), protein expression levels were further examined by immunohistochemistry in xenograft tissues and in 78 radical prostatectomy specimens.
RESULTS
Tumor volume and serum PSA levels were significantly higher in the HFD group than in the LFD group (P < 0.001 and P = 0.006, respectively). We found 64 up‐regulated genes (0.19%) and 14 down‐regulated genes (0.04%) with more than twofold differences in the HFD xenograft. IGF‐IR, TNFRSF, and LPL showed striking differences in the quantitative RT‐PCR experiment. Immunostaining further revealed marked enhanced IGF‐IR expression in the HFD xenograft. In human PCa, the lowest IGF‐IR immunoreactivity group tended to have the lowest body mass index in both normal and PCa epithelium.
CONCLUSION
HFD induced remarkable up‐ and down‐regulation of mRNA of a substantial number of genes. Furthermore, the IGF‐I system may be involved in the HFD‐associated enhanced progression of PCa. Prostate 68: 321–335, 2008. © 2008 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Dietary Fats - administration & dosage</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>high fat diet</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>insulin-like growth factor I receptor</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>microarray</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>prostate cancer</subject><subject>Prostate-Specific Antigen - blood</subject><subject>Prostatic Neoplasms - blood</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Receptor, IGF Type 1 - biosynthesis</subject><subject>Receptor, IGF Type 1 - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Specific Pathogen-Free Organisms</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EotvChQ-AfIFDpZSxncSbI13B8qeiqIA4WpPY3hgSp9hJSw58dxx2KTcu9kjze_PGz4Q8YXDGAPiL6zDEMw7lmt0jKwaVzADy4j5ZAZeQ5UzII3Ic4zeAhAN_SI7YmslCCL4ivzbotdM4Groz3kTq_M3Q3RidCmp8i75J9S4Mt2NLB0vbqUdPF8dx0TRLP9DJ63S2btdSiyO1xmjnd9Rp40dnXZpQz7R3TRgwBJwpeuzm6OIj8sBiF83jw31Cvrx-9XnzJru43L7dvLzImrySLKsL5FBXpa44B2MlWI6sgKYUogEhxTovG6zL3NraSGnQlnWVFzxHresKNRcn5Pl-blr8x2TiqHoXG9N16M0wRSWBgyxykcDTPZhWjTEYq66D6zHMioFawlbL09WfsBP89DB1qnuj_6GHdBPw7ABgbLCzIaXl4h3H02-wsqgSx_bcrevM_B9L9fHq8tNf82yvcXE0P-80GL6rUgpZqK8ftur9u6tzxraVEuI30vKoXw</recordid><startdate>20080215</startdate><enddate>20080215</enddate><creator>Narita, Shintaro</creator><creator>Tsuchiya, Norihiko</creator><creator>Saito, Mitsuru</creator><creator>Inoue, Takamitsu</creator><creator>Kumazawa, Teruaki</creator><creator>Yuasa, Takeshi</creator><creator>Nakamura, Akira</creator><creator>Habuchi, Tomonori</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080215</creationdate><title>Candidate genes involved in enhanced growth of human prostate cancer under high fat feeding identified by microarray analysis</title><author>Narita, Shintaro ; Tsuchiya, Norihiko ; Saito, Mitsuru ; Inoue, Takamitsu ; Kumazawa, Teruaki ; Yuasa, Takeshi ; Nakamura, Akira ; Habuchi, Tomonori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4971-b5a20b96d9220ef70f2a150c633c0373846cab64ffbe77eaf6b94524addb9ad23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Dietary Fats - administration & dosage</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>high fat diet</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>insulin-like growth factor I receptor</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>microarray</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>prostate cancer</topic><topic>Prostate-Specific Antigen - blood</topic><topic>Prostatic Neoplasms - blood</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Receptor, IGF Type 1 - biosynthesis</topic><topic>Receptor, IGF Type 1 - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Narita, Shintaro</creatorcontrib><creatorcontrib>Tsuchiya, Norihiko</creatorcontrib><creatorcontrib>Saito, Mitsuru</creatorcontrib><creatorcontrib>Inoue, Takamitsu</creatorcontrib><creatorcontrib>Kumazawa, Teruaki</creatorcontrib><creatorcontrib>Yuasa, Takeshi</creatorcontrib><creatorcontrib>Nakamura, Akira</creatorcontrib><creatorcontrib>Habuchi, Tomonori</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Narita, Shintaro</au><au>Tsuchiya, Norihiko</au><au>Saito, Mitsuru</au><au>Inoue, Takamitsu</au><au>Kumazawa, Teruaki</au><au>Yuasa, Takeshi</au><au>Nakamura, Akira</au><au>Habuchi, Tomonori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Candidate genes involved in enhanced growth of human prostate cancer under high fat feeding identified by microarray analysis</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2008-02-15</date><risdate>2008</risdate><volume>68</volume><issue>3</issue><spage>321</spage><epage>335</epage><pages>321-335</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><coden>PRSTDS</coden><abstract>BACKGROUND
Several studies have suggested that a high fat diet (HFD) may be a risk factor of prostate cancer (PCa). As a first step to delineate the molecular mechanisms underlying the enhanced progression of PCa under HFD, we investigated the differential gene expressions of a human PCa xenograft under HFD and a low fat diet (LFD).
METHODS
LNCaP cells were subcutaneously injected in 20 nude mice, which were equally divided into two groups, the HFD group and LFD group. Oligonucleotide microarray analyses were performed using mice xenografts from HFD and LFD, and the results of candidate genes with a significant differential expression were validated by quantitative RT‐PCR experiments. As for insulin‐like growth factor I receptor (IGF‐IR), protein expression levels were further examined by immunohistochemistry in xenograft tissues and in 78 radical prostatectomy specimens.
RESULTS
Tumor volume and serum PSA levels were significantly higher in the HFD group than in the LFD group (P < 0.001 and P = 0.006, respectively). We found 64 up‐regulated genes (0.19%) and 14 down‐regulated genes (0.04%) with more than twofold differences in the HFD xenograft. IGF‐IR, TNFRSF, and LPL showed striking differences in the quantitative RT‐PCR experiment. Immunostaining further revealed marked enhanced IGF‐IR expression in the HFD xenograft. In human PCa, the lowest IGF‐IR immunoreactivity group tended to have the lowest body mass index in both normal and PCa epithelium.
CONCLUSION
HFD induced remarkable up‐ and down‐regulation of mRNA of a substantial number of genes. Furthermore, the IGF‐I system may be involved in the HFD‐associated enhanced progression of PCa. Prostate 68: 321–335, 2008. © 2008 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18175332</pmid><doi>10.1002/pros.20681</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Dietary Fats - administration & dosage Gene Expression Regulation, Neoplastic Gynecology. Andrology. Obstetrics high fat diet Humans Immunohistochemistry insulin-like growth factor I receptor Male Male genital diseases Medical sciences Mice Mice, Inbred BALB C Mice, Nude microarray Nephrology. Urinary tract diseases Oligonucleotide Array Sequence Analysis prostate cancer Prostate-Specific Antigen - blood Prostatic Neoplasms - blood Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Receptor, IGF Type 1 - biosynthesis Receptor, IGF Type 1 - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Specific Pathogen-Free Organisms Tumors Tumors of the urinary system Urinary tract. Prostate gland |
| title | Candidate genes involved in enhanced growth of human prostate cancer under high fat feeding identified by microarray analysis |
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