Influence of massed and distributed context preexposure on contextual fear and Egr-1 expression in the basolateral amygdala

Abstract Preexposure to the conditioning context can influence the expression of context-conditioned fear. We used behavioral and early growth response gene (egr-1) assays in rats to study the effects of massed and distributed context preexposure on context-conditioned fear. The results demonstrated that massed context preexposure impaired acquisition of contextual fear, an effect here referred to as delayed shock deficit. Spaced context preexposure produced similar inhibitory effects. Significantly, the introduction of a brief change of context prior to conditioning completely reversed the deficit induced by massed, but not by distributed, context preexposure. This reversibility was inversely related to the duration of the context shift. The acquisition of context-conditioned fear was associated with enhanced Egr-1 expression in the basolateral amygdala (BLA). No such increase was evident in animals undergoing distributed context preexposure or in those experiencing massed preexposure without change of context. Remarkably, a brief change of context prior to conditioning not only facilitated learning following massed preexposure but also elicited a significant elevation of Egr-1 protein levels in the BLA. The findings shown demonstrated that the inhibitory effects of massed and distributed context preexposure on conditioning could be dissociable both behaviorally and physiologically. We suggest that the delayed shock deficit associated with massed preexposure derives from perceptual fade-out or inattention and its reversal by a brief change of context from attentional recovery.