NTA-mediated protein capturing strategy in screening experiments for small organic molecules by surface plasmon resonance

Nitrilotriacetate (NTA)‐mediated capture of a histidine‐tagged protein is widely used as an easy and simple method to reversibly immobilize the protein onto a sensor chip for surface plasmon resonance (SPR). However, in spite of its advantages, the NTA‐capturing strategy is rarely employed for ligan...

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Veröffentlicht in:	Proteomics (Weinheim) 2007-02, Vol.7 (4), p.494-499
Hauptverfasser:	Yoshitani, Naoei, Saito, Kazuki, Saikawa, Wakana, Asanuma, Miwako, Yokoyama, Shigeyuki, Hirota, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Binding Sites Biological and medical sciences Biosensing Techniques Fundamental and applied biological sciences. Psychology Histidine - chemistry Histidine-tagged protein Humans Ligand screening Miscellaneous Nitrilotriacetate Nitrilotriacetic Acid - chemistry Peptides - analysis Peptides - chemistry Phosphatidylinositol 3-Kinases - chemistry Proteins SH3 domain src Homology Domains - physiology Surface Plasmon Resonance
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