Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity
Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced K...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2008, Vol.77 (6), p.1269-1277 |
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| creator | Raimondi, S Uccelletti, D Matteuzzi, D Pagnoni, U. M Rossi, M Palleschi, C |
| description | Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shares 86% and 71% of identity with Kluyveromyces lactis and Saccharomyces cerevisiae Sod1p, respectively. The characteristic motifs and the amino acid residues involved in coordinating copper and zinc and in enzymatic function were conserved. To the aim of developing a microbial production of Cu/Zn superoxide dismutase, we engineered the K. marxianus L3 strain with the multicopy plasmid YG-KmSOD1 harboring the KmSOD1 gene. The production of KmSOD1p in K. marxianus L3 and K. marxianus L3 (pYG-KmSOD1) in response to different compositions of the culture medium was evaluated. The highest specific activity (472 USOD mgprot -¹) and the highest volumetric yield (8.8 x 10⁵ USOD l-¹) were obtained by the recombinant strain overexpressing KmSOD1 in the presence of Cu²⁺ and Zn²⁺ supplements to the culture media. The best performing culture conditions were positively applied to a laboratory scale fed-batch process reaching a volumetric yield of 1.4 x 10⁶ USOD l-¹. |
| doi_str_mv | 10.1007/s00253-007-1270-8 |
| format | Article |
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The production of KmSOD1p in K. marxianus L3 and K. marxianus L3 (pYG-KmSOD1) in response to different compositions of the culture medium was evaluated. The highest specific activity (472 USOD mgprot -¹) and the highest volumetric yield (8.8 x 10⁵ USOD l-¹) were obtained by the recombinant strain overexpressing KmSOD1 in the presence of Cu²⁺ and Zn²⁺ supplements to the culture media. The best performing culture conditions were positively applied to a laboratory scale fed-batch process reaching a volumetric yield of 1.4 x 10⁶ USOD l-¹.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-007-1270-8</identifier><identifier>PMID: 18040680</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Amino Acid Sequence ; Amino acids ; Antibiotics ; Biological and medical sciences ; Bioreactors ; Biotechnologically Relevant Enzymes and Proteins ; Biotechnology ; Carbon ; Cloning, Molecular ; Cu/Zn superoxide dismutase ; Culture media ; Culture Media - chemistry ; E coli ; Enzymes ; Expression ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Dosage ; Gene expression ; Industrial Microbiology ; Industrial production ; Kluyveromyces - enzymology ; Kluyveromyces lactis ; Kluyveromyces marxianus ; Laboratories ; Life Sciences ; Microbial Genetics and Genomics ; Microbiology ; Molecular Sequence Data ; Oxidation ; Oxidative stress ; Plasmids ; Productivity ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins - genetics ; Sequence Alignment ; Sequence Homology, Amino Acid ; Studies ; Superoxide Dismutase - chemistry ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; Yeast</subject><ispartof>Applied microbiology and biotechnology, 2008, Vol.77 (6), p.1269-1277</ispartof><rights>Springer-Verlag 2007</rights><rights>2008 INIST-CNRS</rights><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-dbe03728947471496081f94d65f6de24160ca9bc7e9a35669fdc6047fe04c67e3</citedby><cites>FETCH-LOGICAL-c497t-dbe03728947471496081f94d65f6de24160ca9bc7e9a35669fdc6047fe04c67e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-007-1270-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-007-1270-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19965239$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18040680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raimondi, S</creatorcontrib><creatorcontrib>Uccelletti, D</creatorcontrib><creatorcontrib>Matteuzzi, D</creatorcontrib><creatorcontrib>Pagnoni, U. M</creatorcontrib><creatorcontrib>Rossi, M</creatorcontrib><creatorcontrib>Palleschi, C</creatorcontrib><title>Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shares 86% and 71% of identity with Kluyveromyces lactis and Saccharomyces cerevisiae Sod1p, respectively. The characteristic motifs and the amino acid residues involved in coordinating copper and zinc and in enzymatic function were conserved. To the aim of developing a microbial production of Cu/Zn superoxide dismutase, we engineered the K. marxianus L3 strain with the multicopy plasmid YG-KmSOD1 harboring the KmSOD1 gene. The production of KmSOD1p in K. marxianus L3 and K. marxianus L3 (pYG-KmSOD1) in response to different compositions of the culture medium was evaluated. The highest specific activity (472 USOD mgprot -¹) and the highest volumetric yield (8.8 x 10⁵ USOD l-¹) were obtained by the recombinant strain overexpressing KmSOD1 in the presence of Cu²⁺ and Zn²⁺ supplements to the culture media. The best performing culture conditions were positively applied to a laboratory scale fed-batch process reaching a volumetric yield of 1.4 x 10⁶ USOD l-¹.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Antibiotics</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnologically Relevant Enzymes and Proteins</subject><subject>Biotechnology</subject><subject>Carbon</subject><subject>Cloning, Molecular</subject><subject>Cu/Zn superoxide dismutase</subject><subject>Culture media</subject><subject>Culture Media - chemistry</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Expression</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Dosage</subject><subject>Gene expression</subject><subject>Industrial Microbiology</subject><subject>Industrial production</subject><subject>Kluyveromyces - enzymology</subject><subject>Kluyveromyces lactis</subject><subject>Kluyveromyces marxianus</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Oxidation</subject><subject>Oxidative stress</subject><subject>Plasmids</subject><subject>Productivity</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Studies</subject><subject>Superoxide Dismutase - chemistry</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Yeast</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU2P0zAQhi0EYsvCD-ACFhLcAmPH8ccRlU9RaQ_Lni3XmXS9ykexk2qL-PE4SlElDnCakfy878z4JeQ5g7cMQL1LALwqi9wWjCso9AOyYqLkBUgmHpIVMFUVqjL6gjxJ6Q6AcS3lY3LBNAiQGlbk1_rWRedHjOGnG8PQ06Gh4y3SNO0xDvehRlqH1E2jS0ivrz4wusMeZ-pbOx0PmemOHhPtXLwPrp8S3ZTU9TUN3T4OB6xpLvXk_3hnC5rnhUMYj0_Jo8a1CZ-d6iW5-fTx-_pLsbn6_HX9flN4YdRY1FuEUnFthBKKCSNBs8aIWlaNrJELJsE7s_UKjSsrKU1TewlCNQjCS4XlJXmz-OZVfkyYRtuF5LFtXY_DlKwCDlxy-V-QgylBAWTw1V_g3TDFPh9hOTdSgRI6Q2yBfBxSitjYfQz5n46WgZ0DtEuAdm7nAO2seXEynrYd1mfFKbEMvD4BLnnXNtH1PqQzZ4yseGkyxxcu5ad-h_G84b-mv1xEjRus28VsfHPNgZUAWgihdPkb1hm70g</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Raimondi, S</creator><creator>Uccelletti, D</creator><creator>Matteuzzi, D</creator><creator>Pagnoni, U. 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Psychology</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Dosage</topic><topic>Gene expression</topic><topic>Industrial Microbiology</topic><topic>Industrial production</topic><topic>Kluyveromyces - enzymology</topic><topic>Kluyveromyces lactis</topic><topic>Kluyveromyces marxianus</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Oxidation</topic><topic>Oxidative stress</topic><topic>Plasmids</topic><topic>Productivity</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Studies</topic><topic>Superoxide Dismutase - chemistry</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raimondi, S</creatorcontrib><creatorcontrib>Uccelletti, D</creatorcontrib><creatorcontrib>Matteuzzi, D</creatorcontrib><creatorcontrib>Pagnoni, U. 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M</au><au>Rossi, M</au><au>Palleschi, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2008</date><risdate>2008</risdate><volume>77</volume><issue>6</issue><spage>1269</spage><epage>1277</epage><pages>1269-1277</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shares 86% and 71% of identity with Kluyveromyces lactis and Saccharomyces cerevisiae Sod1p, respectively. The characteristic motifs and the amino acid residues involved in coordinating copper and zinc and in enzymatic function were conserved. To the aim of developing a microbial production of Cu/Zn superoxide dismutase, we engineered the K. marxianus L3 strain with the multicopy plasmid YG-KmSOD1 harboring the KmSOD1 gene. The production of KmSOD1p in K. marxianus L3 and K. marxianus L3 (pYG-KmSOD1) in response to different compositions of the culture medium was evaluated. The highest specific activity (472 USOD mgprot -¹) and the highest volumetric yield (8.8 x 10⁵ USOD l-¹) were obtained by the recombinant strain overexpressing KmSOD1 in the presence of Cu²⁺ and Zn²⁺ supplements to the culture media. The best performing culture conditions were positively applied to a laboratory scale fed-batch process reaching a volumetric yield of 1.4 x 10⁶ USOD l-¹.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18040680</pmid><doi>10.1007/s00253-007-1270-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Amino acids Antibiotics Biological and medical sciences Bioreactors Biotechnologically Relevant Enzymes and Proteins Biotechnology Carbon Cloning, Molecular Cu/Zn superoxide dismutase Culture media Culture Media - chemistry E coli Enzymes Expression Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - metabolism Gene Dosage Gene expression Industrial Microbiology Industrial production Kluyveromyces - enzymology Kluyveromyces lactis Kluyveromyces marxianus Laboratories Life Sciences Microbial Genetics and Genomics Microbiology Molecular Sequence Data Oxidation Oxidative stress Plasmids Productivity Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Sequence Alignment Sequence Homology, Amino Acid Studies Superoxide Dismutase - chemistry Superoxide Dismutase - genetics Superoxide Dismutase - metabolism Yeast |
| title | Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity |
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